We narrowed to 50,933 results for: son

1. SLK_HUMAN_D0

   Plasmid

   #79733

   Purpose

   This plasmid encodes the kinase domain of SLK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   SLK (SLK Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA-Cx43

   Plasmid

   #60691

   Purpose

   mammalian expression of rat Cx43

   Depositor

   Parmender Mehta

   Article

   Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

   Insert

   Cx43 (Gja1 Rat)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pBp-FGFR2b-K660N

   Plasmid

   #45707

   Depositor

   Matthew Meyerson

   Article

   Liao et al Cancer Res. 2013 Jun 20.

   Insert

   FGFR2 IIIb (FGFR2 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   K660N

   Available Since

   June 24, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pTER E2F2shRNA human

   Plasmid

   #66884

   Purpose

   Doxycycline-regulated mammalian expression vector for expressing shRNA against E2F2

   Depositor

   Maria Alvarado-Kristensson

   Article

   Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

   Insert

   E2F transcription factor 2 (E2F2 Human)
   

   Use

   RNAi

   Expression

   Mammalian

   Promoter

   H1 Tet

   Available Since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pSL0747 (pDonor_L-MmeI)

   Plasmid

   #130647

   Purpose

   Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated left end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchCAST donor DNA (L*)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Mutation

   TGTTGATG --> TGTTGGAG

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSL0746 (pDonor_R-MmeI)

   Plasmid

   #130646

   Purpose

   Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated right end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchCAST donor DNA (R*)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Mutation

   TGTTGATA --> TGTTGGAA

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pL90-Nat-2xHO

   Plasmid

   #139069

   Purpose

   CRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selection

   Depositor

   Gianni Liti

   Article

   Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

   Insert

   Two guide RNAs targeting the HO endonuclease
   

   Expression

   Bacterial and Yeast

   Promoter

   TDH3

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. iBE3 CLYBL

   Plasmid

   #174569

   Purpose

   doxycycline inducible BE3 expression for human CLYBL locus knock-in

   Depositor

   Mathew Garnett

   Article

   Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

   Insert

   BE3
   

   Expression

   Mammalian

   Promoter

   tet ON

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAG8H-EWS_1-264

   Plasmid

   #180467

   Purpose

   Expresses the construct His8-EWS_1-264 with a TEV cleavage site for the removal of the His8 tag

   Depositor

   David Libich

   Article

   Johnson et al Biomol NMR Assign. 2022 Apr;16(1):67-73.

   Insert

   EWSR1 (EWSR1 Human)
   

   Tags

   His8

   Expression

   Bacterial

   Mutation

   deleted 265-656

   Promoter

   T7

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5-FRT-TO-Nsp5

    Plasmid

    #157685

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp5 (ORF1ab SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    codon optimized

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pcDNA-Cx43-Myc

    Plasmid

    #60693

    Purpose

    mammalian expression of Myc-tagged rat Cx43

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA5-FRT-TO-FH-Nsp1

    Plasmid

    #157680

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp1 (ORF1ab SARS-CoV-2)
    

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Available Since

    Aug. 4, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pETM33_CEP55_EABR

    Plasmid

    #178469

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    CEP55_EABR (CEP55 Human, Synthetic)
    

    Tags

    His-GST

    Expression

    Bacterial

    Promoter

    T7/LacO

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. LXSN-Cx43

    Plasmid

    #60692

    Purpose

    retroviral expression of rat Cx43

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA5-FRT-TO-Nsp1-HF

    Plasmid

    #157681

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    HF-Nsp1 (ORF1ab SARS-CoV-2)
    

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized

    Available Since

    Aug. 4, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pTER E2F3shRNA human

    Plasmid

    #66885

    Purpose

    Doxycycline-regulated mammalian expression vector for expressing shRNA against E2F3

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    E2F transcription factor 3 (E2F3 Human)
    

    Use

    RNAi

    Expression

    Mammalian

    Promoter

    H1 Tet

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pETM33_EPS15_EH

    Plasmid

    #178475

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    EPS15_EH (EPS15 Human, Synthetic)
    

    Tags

    His-GST

    Expression

    Bacterial

    Promoter

    T7/LacO

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRII-Blunt-U6-HES7-STOP-gRNA

    Plasmid

    #130933

    Purpose

    gRNA for tag human HES7

    Depositor

    James Thomson

    Article

    Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

    Insert

    HES7-STOP-gRNA (HES7 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-FRT-TO-Nsp7-HF

    Plasmid

    #157690

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp7-HF (ORF1ab SARS-CoV-2)
    

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pEGFPN1-N-gamma-tubulin (1-333)

    Plasmid

    #87858

    Purpose

    Fluorescent fragment of gamma-tubulin that lacks gamma-tubulin DNA binding domain and NLS

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    gamma-tubulin 1 (TUBG1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Fragment from aa 1 to 333

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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