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We narrowed to 28,320 results for: REP

Showing: 3781 - 3810 of 28320 results

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pET32a-hEGF

Plasmid

#199231

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Epidermal growth factor, partial (EGF Human)

Use

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+(Zeo)-FLAG-muGFP

Plasmid

#166726

Purpose

Expresses FLAG-tagged monomeric ultrastable GFP (muGFP) in mammalian cells

Depositor

Daniel Scott

Article

Scott et al Sci Rep. 2018 Jan 12;8(1):667. doi: 10.1

Insert

muGFP

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG_mup53_Ex4

Plasmid

#85534

Purpose

Inducible expression of guide RNA (mup53_Ex4) with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

mu p53

Use

Tags

Expression

Mutation

Promoter

H1t

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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PGK ARF6(Q67L)-CFP

Plasmid

#200428

Purpose

Mammalian expression of Q67L mutation in ARF6

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

ARF6 (ARF6 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Q67L

Promoter

PGK

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBJB20 - eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR

Plasmid

#158967

Purpose

Ubiquitous expression of mtON in C. elegans

Depositor

Andrew Wojtovich

Article

Berry et al EMBO Rep. 2020 Apr 3;21(4):e49113. doi:

Insert

eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

eft-3

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR

Plasmid

#162334

Purpose

Lentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

dSaCas9-KRAB

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-Y530F

Plasmid

#140318

Purpose

Expresses HA-tagged human Src-Y530F in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Use

Tags

2X HA

Expression

Mammalian

Mutation

Y530F

Promoter

CMV

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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hsp70: mKate2-DN Kif5a

Plasmid

#105972

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

Insert

Kif5a (kif5aa Zebrafish)

Use

Tags

mKate2

Expression

Bacterial

Mutation

truncation of aa416-1033

Promoter

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pJRH051

Plasmid

#171625

Purpose

2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-BsmBI-Destination
mNeon

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1-a and U6

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual_FluA/H7N9

Plasmid

#229860

Purpose

pFastBac Dual vector for co-expression of FluPolA/H7N9 polymerase heterotrimer (PA/PB1/PB2)

Depositor

Stephen Cusack

Article

Kouba et al Cell Rep. 2023 Jan 31;42(1):111901. doi:

Inserts

PA_H7N9-FluA
PB1_H7N9-FluA
PB2_H7N9-FluA

Use

Tags

2x-Streptag and 8xHis-tag

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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LT3REVIR

Plasmid

#111176

Purpose

Tet-ON miR-E (miR-30 variant)-based RNAi

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Electra2-P2A-EGFP

Plasmid

#184936

Purpose

Expresses blue fluorescent protein Electra2 and EGFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra2-P2A-EGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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ER-ABKAR

Plasmid

#61508

Purpose

Expression of AMPK biosensor targeted to the endoplasmic reticulum

Depositor

Jin Zhang, Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Use

Tags

ER targeting sequence from CYP450 2C1 (see commen…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-mTagBFP2-P2A-EGFP

Plasmid

#184943

Purpose

Expresses blue fluorescent protein mTagBFP2 and EGFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

mTagBFP2-P2A-EGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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-5.5kb-UCP1-GFP

Plasmid

#104585

Purpose

Reporter assay to monitor the transcription activity of the UCP1 promoter by GFP expression

Depositor

Irfan Lodhi

Article

Lodhi et al Cell Rep. 2017 Sep 19;20(12):2766-2774.

Insert

UCP1 promoter (Ucp1 Mouse)

Use

Tags

TurboGFP

Expression

Mammalian

Mutation

Promoter

UCP1

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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CLYBL-TO-PAX7

Plasmid

#204346

Purpose

Knock-in vector to insert a dox-inducible PAX7 transgene into the CYBL locus in human pluripotent stem cells. PAX7 induction results in myogenic differentiation.

Depositor

Ivo Lieberam

Article

Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

Insert

human PAX7

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pK037.CAG-FRT-stop-FRT-RFP-ires-tTA-WPRE (Supernova)

Plasmid

#85038

Purpose

For Flpe/FRT-based Supernova-RFP, pK037 should be used with pK036.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

FRT-stop-FRT

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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PB-TRE-GNL

Plasmid

#204499

Purpose

Expresses Glis1, Nanog and lin28a by tet-on promoter in mammalian cells

Depositor

Wataru Fujii

Article

Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

Insert

Glis1 (Glis1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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PM-ABKAR

Plasmid

#61506

Purpose

Expression of AMPK biosensor targeted to the plasma membrane via Lyn kinase targeting sequence

Depositor

Jin Zhang, Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Use

Tags

Lyn kinase sequence (see comments)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pDEST40-2XHA-wtSrc

Plasmid

#140294

Purpose

Expresses HA-tagged wild-type human Src in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src wildtype (SRC Human)

Use

Tags

2X HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pXJ-Myc-KLF2

Plasmid

#232654

Purpose

transient expresses KLF2 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

KLF2 (KLF2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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RT3GEPIR

Plasmid

#111169

Purpose

Tet-regulated miR-E (miR-30 variant)-based RNAi

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Retroviral

Tags

Expression

Mutation

WT

Promoter

T3G

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRRLSIN.cPPT.PGK.MD(ND2bHLH).WPRE

Plasmid

#122055

Purpose

Lentiviral expression of a MyoD chimeric protein substituted with the NeuroD2 bHLH domain

Depositor

Stephen Tapscott

Article

Fong et al Cell Rep. 2015 Mar 31;10(12):1937-46. do

Insert

MD(ND2bHLH) (Myod1, Neurod2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

MyoD chimeric protein substituted with the NeuroD…

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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1000.pCCLsin.cPPT.hPGK.Ascl1.WPRE

Plasmid

#67291

Purpose

Induce human iN conversion from fibroblast

Depositor

Malin Parmar

Article

Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

Insert

Asc1 (Ascl1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pDN34-BiNLS-hYAP1-1delta

Plasmid

#169471

Purpose

expresses optoYAP in mammalian cells

Depositor

Timothy Saunders

Article

Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

Insert

YAP1-1delta (YAP1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti CMV TRE3G BCL2L1 Neo

Plasmid

#140749

Purpose

Inducibly expresses Bcl-xL in mammalian cells

Depositor

Scott Dixon

Article

Inde et al Cell Rep. 2020 Jul 7;32(1):107845. doi:

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV TRE3G

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBR_attB(C31)_FRT

Plasmid

#183761

Purpose

phiC31-specific donor plasmid for the cloning of payloads <20 kb

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pBABR IRFP-Bcd-LEXY

Plasmid

#182595

Purpose

Drosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.

Depositor

Jared Toettcher

Article

Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

Insert

Bcd (bcd Fly)

Use

Tags

IRFP and LEXY

Expression

Insect

Mutation

Promoter

maternal tubulin

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Cox7c-pHLuorin-sems

Plasmid

#107766

Purpose

labeling of mitochondrial cytochrome c oxidase, pH measurements

Depositor

Karin Busch

Article

Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.103

Insert

Subunit Cox7c fused to pHluorin (COX7C Human)

Use

Tags

pHluorin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pSCMV-H2B-FT-Medium

Plasmid

#157670

Purpose

Retroviral vector for expressing chimeric human histone H2B fused with the "Medium" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

Depositor

Shangqin Guo

Article

Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

Insert

Histone H2B

Use

Retroviral

Tags

Medium-FT

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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