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pEB2-2ndVal mScarlet-I

Plasmid

#104028

Purpose

Plasmid encoding 2ndVal mScarlet-I

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mScarlet-I

Use

Low copy

Expression

Bacterial

Mutation

mScarlet-I with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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MS2-Snapf

Plasmid

#65860

Purpose

Expresses RNA-binding protein MS2 with fast SNAP fusion tag

Depositor

Aaron Hoskins

Article

Carrocci et al Analyst. 2014 Jan 7;139(1):44-7. doi: 10

Inserts

MS2
Fast SNAP

Tags

HA and NLS

Expression

Bacterial and Yeast

Available Since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only
pOPING

Plasmid

#26046

Depositor

Ray Owens

Article

Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

Insert

SS peptide-LacZ-KHHHHHH

Tags

KHHHHHH and MGILPSPGMPALLSLVSLLSVLLMGCVAETG

Expression

Bacterial, Insect, and Mamm…

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-345

Plasmid

#68785

Purpose

R->A Sensor pair 1 with mCherry marker

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

C-Terminal Sensor 1 V2 R-->A mutant
N-Terminal Sensor 1 V2 R-->A mutant
mCherry

Tags

NLS, flag, and nls

Expression

Mammalian

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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mSc-sspB

Plasmid

#114421

Purpose

Translocating to iLID domain

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

iLID domain

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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PKC zeta WT

Plasmid

#21248

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC zeta (Prkcz Rat)

Tags

HA

Expression

Mammalian

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only
pGL4.26-SS-352

Plasmid

#68791

Purpose

ZF9 Op x6 -- GFP-IRES-NTR

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

ZF9 Op 6x
EGFP
Nitroreductase

Tags

IRES

Expression

Mammalian

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pMS050

Plasmid

#91826

Purpose

Codon-optimized mScarlet-I donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

mScarlet-I-C1

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal mCherry2-L

Plasmid

#104027

Purpose

Plasmid encoding 2ndVal mCherry2-L

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mCherry2-L

Use

Low copy

Expression

Bacterial

Mutation

mCherry2-L with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pDD378

Plasmid

#91827

Purpose

Codon-optimized HaloTag donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

HaloTag-C1

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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MTST5

Plasmid

#86602

Purpose

Custom designed synthetic DNA fragment lacking homology to natural sequences for preparation of internal standards for RNA-seq in metatranscriptomics.

Depositor

Mary Ann Moran

Article

Satinsky et al Methods Enzymol. 2013;531:237-50. doi: 1

Insert

MTST5

Use

Internal standard reverse transcription

Promoter

T7

Available Since

Sept. 15, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pKAM-E-Flag-BFP

Plasmid

#101867

Purpose

Lentiviral Evi-1-Flag with dual BFP and puro markers

Depositor

Archibald Perkins

Insert

Evi-1 (Mecom Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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NLS-LucDM-GFP

Plasmid

#127796

Purpose

The expressed ORF encodes nucleus location signal with GFP and destabilized mutant Luciferase.

Depositor

Xiaolu Yang

Article

Guo et al J Vis Exp. 2016 Aug 28;(114). doi: 10.37

Insert

destabilized mutant (DM) Luciferase

Tags

EGFP and FLAG-NLS

Expression

Mammalian

Mutation

R188Q, R261Q in Firefly Luciferase insert

Promoter

CMV

Available Since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal mKate2

Plasmid

#104030

Purpose

Plasmid encoding 2ndVal mKate2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mKate2

Use

Low copy

Expression

Bacterial

Mutation

mKate2 with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_ChR2-3M-YFP

Plasmid

#186977

Purpose

Expresses ChR2-3xM channelrhodopsin in mammalian cells

Depositor

Adam Cohen

Article

Hayward et al eLife. 2023 Oct 6;12:RP86833. doi: 10.75

Insert

ChR2-3xM-YFP

Use

Lentiviral

Tags

eYFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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MTST6

Plasmid

#86603

Purpose

Custom designed synthetic DNA fragment lacking homology to natural sequences for preparation of internal standards for RNA-seq in metatranscriptomics.

Depositor

Mary Ann Moran

Article

Satinsky et al Methods Enzymol. 2013;531:237-50. doi: 1

Insert

MTST6

Expression

Bacterial

Promoter

T7

Available Since

Sept. 15, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pUC119 T7 hTRmin

Plasmid

#127858

Purpose

T7 RNA Pol for in vitro transcription

Depositor

Kathleen Collins

Article

Wu et al EMBO J. 2014 Apr 16;33(8):921-35. doi: 1

Insert

hTR (TERC Human)

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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KW63_HART_RAC1_PuroR

Plasmid

#207400

Purpose

Lentiviral plasmid containing DDX3X reporter HART for RAC1 5UTR - PuroR

Depositor

Stephen Floor

Article

Wilkins et al RNA. 2024 Jul 16;30(8):1041-1057. doi: 1

Insert

eGFP

Use

Lentiviral

Expression

Mammalian

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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PKC eta CAT

Plasmid

#21247

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC eta (Prkch Mouse)

Tags

HA

Expression

Mammalian

Mutation

delta 1-341

Available Since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
pEB2-2ndVal Katushka

Plasmid

#104029

Purpose

Plasmid encoding 2ndVal Katushka

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal Katushka

Use

Low copy

Expression

Bacterial

Mutation

Katushka with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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NF-155 pcDNA3

Plasmid

#197290

Purpose

Plasmid expressing an antigen targeting the Plasmid expressing an antigen targeting Neurofascin-155

Depositor

Peter Brophy

Article

Collinson et al Glia. 1998 May;23(1):11-23

Insert

Pan-Neurofascin (extracellular) (Nfasc Rat)

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pDD377

Plasmid

#91823

Purpose

Codon-optimized mTurquoise2 donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

mTurquoise2-C1

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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2xsspB-mSc-sspB

Plasmid

#114423

Purpose

Translocating to iLID domain

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

iLID domain

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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PA-mCherry1-PCNA-19-SV40NLS-4

Plasmid

#57192

Purpose

Localization: Nucleus/Replication Origins, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Insert

PCNA-19 SV40NLS (PCNA Human)

Tags

3x NLS and PA-mCherry1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal Kat9-5

Plasmid

#104031

Purpose

Plasmid encoding 2ndVal Katushka9-5

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal Katushka9-5

Use

Low copy

Expression

Bacterial

Mutation

Katushka9-5 with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal mCherry-L

Plasmid

#104026

Purpose

Plasmid encoding 2ndVal mCherry-L

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mCherry-L

Use

Low copy

Expression

Bacterial

Mutation

mCherry-L with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PKC eta dNPS

Plasmid

#21245

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC eta (Prkch Mouse)

Tags

HA

Expression

Mammalian

Mutation

delta 1-165

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only
pMON-HsNMT2

Plasmid

#67476

Purpose

Expression of human N-myristoyltransferase 2 for N-myristoylation of ARF family proteins in bacteria

Depositor

Richard Kahn

Article

Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

Insert

HsNMT2 (NMT2 Human)

Expression

Bacterial

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
mKeima-Red-Keratin-17

Plasmid

#56017

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 440, Emission: 620

Depositor

Michael Davidson

Insert

Keratin (KRT18 Human)

Tags

mKeima-Red

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available Since

April 1, 2015

Availability

Academic Institutions and Nonprofits only

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pProm11_BCD1-GFP

Plasmid

#80656

Purpose

Promoter11 with BCD1-gfp

Depositor

Nathan Hillson

Article

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Mutation

NONE

Promoter

constitutive promoter

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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