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Showing: 6301 - 6350 of 13576 results

  15. pCpep-E1GFP

    Plasmid
    #233884
    Purpose
    Expression of a pH sensitive variant of EGFP inserted in the C-peptide of insulin for pH measurement in insulin secretory granules
    Depositor
    Francesco Cardarelli
    Article
    De Lorenzi et al Commun Biol. 2025 Feb 25;8(1):304. doi:
    Insert
    Proinsulin-E1GFP (Ins2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Inserted E1GFP into C-peptide of mouse Ins2 codin…
    Promoter
    CMVF
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233884-plasmid-map-sequence-id-468196

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  16. pHAGE2-EF1a-E1MLS-2xFLAG-T4Lig-IRES-Neo

    Plasmid
    #234953
    Purpose
    Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid
    Depositor
    Agnel Sfeir
    Article
    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.
    Insert
    human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    234953-plasmid-map-sequence-id-467731

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  17. pHAGE2-EF1a-FOATPMLS-2xFLAG-T4Lig-IRES-Neo

    Plasmid
    #234954
    Purpose
    Human codon optimized T4 DNA Ligase with N-terminal F0ATP MLS expressing plasmid
    Depositor
    Agnel Sfeir
    Article
    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.
    Insert
    human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of subunit 9 of F0-ATP synthase
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    234954-plasmid-map-sequence-id-467729

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  22. pLVX-CAPN7-V18D-mCherry

    Plasmid
    #193046
    Purpose
    Lentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with V18D (IST1-binding mutation) with C-terminal mCherry tag.
    Depositor
    Wesley Sundquist
    Article
    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75
    Insert
    CAPN7 (CAPN7 Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    V18D, siRNA resistant to: GCACCCAUACCUUUACAUU
    Promoter
    Tight TRE
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    193046-plasmid-map-sequence-id-379016

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  23. pLVX-CAPN7-C290S-mCherry

    Plasmid
    #193047
    Purpose
    Lentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with C290S (proteolysis inactivating mutation) with C-terminal mCherry tag.
    Depositor
    Wesley Sundquist
    Article
    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75
    Insert
    CAPN7 (CAPN7 Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    C290S, siRNA resistant to: GCACCCAUACCUUUACAUU
    Promoter
    Tight TRE
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    193047-plasmid-map-sequence-id-379019

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  27. pHR-Spy-CD28(CD45TM)

    Plasmid
    #223624
    Purpose
    Lentiviral expression of human CD28 (CD45TM) with SpyTag003 in mammalian cells
    Depositor
    Enfu Hui
    Article
    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1
    Insert
    CD28 (CD28 Human)
    Use
    Lentiviral; mammalian expression
    Tags
    Spytag003
    Expression
    Mutation
    CD28 transmembrane domain replaced by CD45 transm…
    Promoter
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    223624-plasmid-map-sequence-id-444084

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  35. PX459v3-eSpCas9(1.1)

    Plasmid
    #178800
    Purpose
    High-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178800-plasmid-map-sequence-id-354839

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  36. PX459v3-SpCas9-HF1

    Plasmid
    #178801
    Purpose
    High-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178801-plasmid-map-sequence-id-354837

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  37. pTFG001-ACE2(WT)-HA

    Plasmid
    #191569
    Purpose
    ACE2 insert in a 2nd generation lentiviral transfer plasmid (pGIPZ) to generate ACE2(WT)-expressing cells. Codon-optimized for human expression. Insert contains C-terminal HA tag.
    Depositor
    Joshua Leonard
    Article
    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10
    Insert
    Angiotensin Converting Enzyme II (ACE2 Human)
    Use
    Lentiviral and RNAi
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    Codon optimized for expression in human cells
    Promoter
    CMV
    Available since
    Aug. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191569-plasmid-map-sequence-id-441362

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  40. AAV2_CAG_oROS-HT(C199S)_WPRE

    Plasmid
    #216415
    Purpose
    Encodes the loss-of-function mutation C199S of genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectors
    Depositor
    Andre Berndt
    Article
    Lee et al bioRxiv 2024.02.06.579232
    Insert
    oROS-HT(C199S)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216415-plasmid-map-sequence-id-429726

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Showing: 6301 - 6350 of 13576 results