We narrowed to 115,891 results for: mpi

1. pINER5

   Plasmid

   #185895

   Purpose

   In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).

   Depositor

   Claudia Vickers

   Insert

   PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Aug. 25, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pGAL-flp(H305L)-NAT

   Plasmid

   #160380

   Purpose

   overexpression of Flp recombinase step-arrest mutant in yeast

   Depositor

   John McCusker

   Insert

   FLP1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H305L

   Promoter

   GAL

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHH0103 NEDD4 WW domain #1

   Plasmid

   #104208

   Purpose

   Bacterial expression of WW domain #1 from NEDD4

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   NEDD4 WW domain #1 (NEDD4 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available since

   Feb. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLinker-BirA-3xHA-HXGPRT-loxP

   Plasmid

   #86668

   Purpose

   for generation of BirA tagged strains using CRISPR tagging technology

   Depositor

   David Sibley

   Insert

   Linker-BirA-3xHA-HXGPRT 3'UTR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTYB2-eIF1

   Plasmid

   #37217

   Depositor

   Jon Lorsch

   Article

   Acker et al Methods Enzymol. 2007;430:111-45.

   Insert

   eIF1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pGEX-MK2

   Plasmid

   #30187

   Depositor

   Bruce Tempel

   Insert

   MK2 (Kcna2 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pZHY051

   Plasmid

   #44987

   Depositor

   Daniel Voytas

   Article

   Zhang et al Plant Physiol. 2012 Nov 2.

   Type

   Empty backbone

   Use

   Plant expression

   Tags

   Expression

   Mutation

   Promoter

   35S

   Available since

   June 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAVS1_Puro_H2BK_mNG_hSLBP(18-108)

   Plasmid

   #191529

   Purpose

   Donor for targeted integration of mNG_hSLBP(18-108) probe to the AAVS1 safe harbor locus

   Depositor

   Yannick Doyon

   Insert

   H2BK_mNG_hSLBP(18-108)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   mNeonGreen (mNG)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NM-01

   Plasmid

   #69799

   Purpose

   Bacterial expression of Cas9 with His, MBP, and Strep tags

   Depositor

   Gerard Cagney

   Insert

   Cas9
   

   Use

   Tags

   HIS, MBP, and StrepII

   Expression

   Bacterial

   Mutation

   Aspartate 10 to Alanine (D10A) and Histidine 840 …

   Promoter

   Available since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pflaG

    Plasmid

    #45477

    Depositor

    Michele Swanson

    Article

    Hammer et al Mol Microbiol. 1999 Aug;33(4):721-31.

    Insert

    flaA-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    flaA

    Available since

    June 4, 2013

    Availability

    Academic Institutions and Nonprofits only

11. BII-gR-PnTW

    Plasmid

    #133356

    Purpose

    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS

    Depositor

    Blair Madison

    Insert

    tdTomato-NLS
    

    Use

    Tags

    HA-tagged tdTomato

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pscAAV-CBh-tagBFP2-WPRE3-enSV40pA

    Plasmid

    #194246

    Purpose

    Expresses tagBFP2

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    tagBFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CBh

    Available since

    May 8, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pDTA-5

    Plasmid

    #128845

    Purpose

    Gateway-compatible p15A origin plasmid with a DTA negative selection cassette for targeting vector construction, I-CeuI, low copy number

    Depositor

    Mario Capecchi

    Type

    Empty backbone

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX551-miniCMV-CjCas9

    Plasmid

    #107033

    Purpose

    AAV plasmid expressing CjCas9 under control of miniCMV promoter

    Depositor

    Alex Hewitt

    Insert

    CjCas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAS1-rab-3P::his-24::CyOFP

    Plasmid

    #124347

    Purpose

    Nuclear Localized Pan-Neuronal CyOFP

    Depositor

    Andrew Leifer

    Insert

    rab-3, CyOFP (rab-3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX551-miniCMV-SaCas9

    Plasmid

    #107032

    Purpose

    AAV plasmid expressing SaCas9 under control of miniCMV promoter

    Depositor

    Alex Hewitt

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHH0103 NEDD4 WW domain #4

    Plasmid

    #104211

    Purpose

    Bacterial expression of WW domain #4 from NEDD4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4 WW domain #4 (NEDD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK633

    Plasmid

    #72397

    Purpose

    Produces Escherichia coli aminoimidazole ribonucleotide synthetase, N-terminal His6 tag (H6EcPurM)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole synthetase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TetCMV-Pfv-Sapphire

    Plasmid

    #203650

    Purpose

    Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the doxycycline-inducible promoter.

    Depositor

    Liam Holt

    Article

    Shu T et et al bioRxiv 2022

    Insert

    PfV
    

    Use

    Lentiviral

    Tags

    Sapphire

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAd:TRE-gateway

    Plasmid

    #30217

    Depositor

    William Pu

    Article

    He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

21. pCSegfpcherry

    Plasmid

    #22465

    Depositor

    Nathan Lawson

    Article

    Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

    Insert

    EGFP-mCherry
    

    Use

    Injection construct

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKSB- 3xP3-GFPsv40 GGGG AAGA

    Plasmid

    #174531

    Purpose

    3xP3-GFP fluorescence marker cassette for gene knock-in in mosquitoes

    Depositor

    Eric Marois

    Insert

    3xP3-GFP BsaI cassette
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    3xP3

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHACK-GAL4>GAL80

    Plasmid

    #104874

    Purpose

    HACK Donor plasmids for converting GAL4 lines to GAL80 lines using HACK method.

    Depositor

    Christopher Potter

    Insert

    T2A-GAL80
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Tobacco Etch Virus NIa protease (GB0420)

    Plasmid

    #160553

    Purpose

    pUPD_NIaPro(TEV)

    Depositor

    Diego Orzaez

    Insert

    Tobacco Etch Virus NIa protease
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAV-syn-NES-jGCaMP8m-WPRE

    Plasmid

    #186036

    Purpose

    AAV-mediated cytosolic expression of jGCaMP8m under the synapsin promoter

    Depositor

    Loren Looger

    Insert

    NES-jGCaMP8m
    

    Use

    AAV

    Tags

    NES-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PB CAG-sfGFP-iCre-ERT2

    Plasmid

    #137856

    Purpose

    CAG-sfGFP-iCre-ERT2 piggyBac transposon

    Depositor

    Mario Capecchi

    Insert

    sfGFP-iCre-ERT2
    

    Use

    Cre/Lox; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EMSV-MRF4 (puro)

    Plasmid

    #14713

    Depositor

    Michael Rudnicki

    Article

    Sabourin et al Clin Genet. 2000 Jan . 57(1):16-25.

    Insert

    MRF4 (Myf6 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2007

    Availability

    Academic Institutions and Nonprofits only

28. pTKIP-dhfr

    Plasmid

    #41067

    Depositor

    Edward Cox, Thomas Kuhlman

    Article

    Kuhlman et al Nucleic Acids Res. 2010 Apr;38(6):e92. E

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CbSBS-Flag/TRRAP(S722F)

    Plasmid

    #32105

    Depositor

    Yardena Samuels

    Insert

    Transformation/transcription domain-associated protein (S722F) (TRRAP Human)
    

    Use

    Tags

    Flag 1x

    Expression

    Mammalian

    Mutation

    Changes serine 722 to Phe

    Promoter

    CMV

    Available since

    Nov. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

30. pTH278-GST-TEV-SUP45

    Plasmid

    #29743

    Depositor

    Tobias von der Haar

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    wild type

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AAV-syn-NES-jGCaMP8s-WPRE

    Plasmid

    #186037

    Purpose

    AAV-mediated cytosolic expression of jGCaMP8f under the synapsin promoter

    Depositor

    Loren Looger

    Insert

    NES-jGCaMP8s
    

    Use

    AAV

    Tags

    NES-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTYB2-eIF5

    Plasmid

    #37219

    Depositor

    Jon Lorsch

    Article

    Acker et al Methods Enzymol. 2007;430:111-45.

    Insert

    eIF5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

33. pLS-CG

    Plasmid

    #12161

    Depositor

    Inder Verma

    Article

    Miyoshi et al J Virol. 1998 Oct . 72(10):8150-7.

    Insert

    CMV-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV-luc-miR21(P)

    Plasmid

    #20382

    Depositor

    Bryan Cullen

    Article

    Zeng et al RNA. 2003 Jan . 9(1):112-23.

    Insert

    mir21(P) targets
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

35. pCDNA3-hTICAM1-VenusFlag

    Plasmid

    #197632

    Purpose

    Human TRIF protein expression in mammalian cells

    Depositor

    Haian Fu

    Insert

    TICAM1 (TICAM1 Human)
    

    Use

    Tags

    Venus-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.1 myc-PITPalpha

    Plasmid

    #58277

    Purpose

    mammalian expression of human PITP alpha

    Depositor

    Shamshad Cockcroft

    Insert

    PITP alpha (PITPNA Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

37. pX601-miniCMV-SaCas9-U6-YFPvsSaCas9 sgRNA

    Plasmid

    #107050

    Purpose

    All-in-one vectors expressing both SaCas9 and YFP sgRNA

    Depositor

    Alex Hewitt

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEB355

    Plasmid

    #22070

    Depositor

    Emmanuelle Bouveret

    Article

    Gully et al Proteomics. 2006 Jan . 6(1):282-93.

    Type

    Empty backbone

    Use

    Tags

    T18

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1-hPMCA4b

    Plasmid

    #47595

    Depositor

    Emanuel Strehler

    Insert

    PMCA4b (ATP2B4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

40. pCMV-luc-random

    Plasmid

    #20877

    Depositor

    Bryan Cullen

    Article

    Zeng et al RNA. 2003 Jan . 9(1):112-23.

    Insert

    random sequence targets
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

41. pcDNA3.1-hRIPK1-Flag-S320A

    Plasmid

    #112491

    Purpose

    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

    Depositor

    Junying Yuan

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S320A

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. p416-GPD-PRUNE-HA

    Plasmid

    #183945

    Purpose

    Expresses human HA-tagged PRUNE from yeast GPD promoter

    Depositor

    Michael Downey

    Insert

    PRUNE (PRUNE1 Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    WT

    Promoter

    GPD

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pB6-ROSA26-DTA-Capecchi-for

    Plasmid

    #125743

    Purpose

    B6J ROSA26 targeting vector homology arms, Capecchi configuration, DTA, I-CeuI, forward cloning sites, low copy number

    Depositor

    Mario Capecchi

    Type

    Empty backbone

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. Pv-FDPS

    Plasmid

    #25554

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    Pv-FDPS
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

45. pET21a(+)_C.jejuni m23 peptidase

    Plasmid

    #83297

    Purpose

    Expresses m23 peptidase from Campylobacter jejuni

    Depositor

    SeWon Suh

    Insert

    ERS445056_01343
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-20

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCFPT7

    Plasmid

    #28012

    Depositor

    Lingchong You

    Article

    Tan et al Nat Chem Biol. 2009 Nov . 5(11):842-8.

    Insert

    T7 RNAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. MT4_C30Y R31W

    Plasmid

    #105712

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 3 subisoform with C30Y and R31W substitution

    Depositor

    Artur Krężel

    Insert

    Metallothionein 4 (MT4 Human)
    

    Use

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Mutation

    This subisoform has a C30Y and R31W substitution

    Promoter

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pK-11

    Plasmid

    #22094

    Depositor

    Gail Martin

    Article

    Meyers et al Nat Genet. 1998 Feb . 18(2):136-41.

    Insert

    Frt-PGKNeo-Frt-LoxP
    

    Use

    Bacterial

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

49. pAS1-rab-3P::his24::tagBFP2

    Plasmid

    #124344

    Purpose

    Nuclear Localized Pan-Neuronal tagBFP2

    Depositor

    Andrew Leifer

    Insert

    rab3, tagBFP2 (rab-3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pTKIP-cat

    Plasmid

    #41065

    Depositor

    Edward Cox, Thomas Kuhlman

    Article

    Kuhlman et al Nucleic Acids Res. 2010 Apr;38(6):e92. E

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge