We narrowed to 2,604 results for: ubiquitin

1. pOPINK-TRABID (NZF1-3, aa 1-178)

   Plasmid

   #66185

   Purpose

   Expresses TRABID NZF1-3 (aa 1-178) in E. coli

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

   Insert

   TRABID (ZRANB1 Human)
   

   Use

   Tags

   His6-GST

   Expression

   Bacterial

   Mutation

   only codes for amino acids 1-178 of TRABID

   Promoter

   T7

   Available since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

2. Human USP30 (64-502, construct 13i, C77A, MG-31-28)

   Plasmid

   #110747

   Purpose

   Bacterial expression of human USP30 (construct 13i)

   Depositor

   David Komander

   Article

   Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

   Insert

   USP30 (USP30 Human)
   

   Use

   Tags

   N-His6-GST-3C

   Expression

   Bacterial

   Mutation

   C77A, F348D, M350S, I353E + insertion deletion: 6…

   Promoter

   T7

   Available since

   Aug. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET-NEMOL329P

   Plasmid

   #11969

   Depositor

   Jon Ashwell

   Article

   Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

   Insert

   NEMOL329P (IKBKG Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Leucine329 to Proline

   Promoter

   Available since

   July 13, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pGST1-GGA1-VHS

   Plasmid

   #44418

   Depositor

   James Hurley

   Article

   Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

   Insert

   GGA1-VHS (1-148) (GGA1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pGST1-GGA2-VHS

   Plasmid

   #44419

   Depositor

   James Hurley

   Article

   Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

   Insert

   GGA2-VHS (GGA2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Alanine 23 changed to Threonine

   Promoter

   Available since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pAM59

   Plasmid

   #227655

   Purpose

   pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, Δ78–81)

   Depositor

   Andreas Martin

   Article

   Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-

   Inserts

   Rpn11 (RPN11 Budding Yeast)
   Rpn8 (RPN8 Budding Yeast)
   

   Use

   Tags

   H6-PreScission and PreScission-Strep

   Expression

   Bacterial

   Mutation

   1-179 and amino acids 2-239, Δ78–81

   Promoter

   T7

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAM57

   Plasmid

   #227654

   Purpose

   pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, V80A)

   Depositor

   Andreas Martin

   Article

   Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-

   Inserts

   Rpn11 (RPN11 Budding Yeast)
   Rpn8 (RPN8 Budding Yeast)
   

   Use

   Tags

   H6-PreScission and PreScission-Strep

   Expression

   Bacterial

   Mutation

   1-179 and amino acids 2-239, V80A

   Promoter

   T7

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAM54

   Plasmid

   #227653

   Purpose

   pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, L132A)

   Depositor

   Andreas Martin

   Article

   Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-

   Inserts

   Rpn11 (RPN11 Budding Yeast)
   Rpn8 (RPN8 Budding Yeast)
   

   Use

   Tags

   H6-PreScission and PreScission-Strep

   Expression

   Bacterial

   Mutation

   1-179 and amino acids 2-239, L132A

   Promoter

   T7

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAM71

   Plasmid

   #227621

   Purpose

   pETDuet-1 H6-precission-Rpn11(24-160-GGSGSG-192-221) , Rpn8(1-179)

   Depositor

   Andreas Martin

   Article

   Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d

   Inserts

   Rpn11 (RPN11 Budding Yeast)
   rpn8 (RPN8 Budding Yeast)
   

   Use

   Tags

   His-Prescission

   Expression

   Bacterial

   Mutation

   1-179 and amino acids 24-160-GGSGSG-192-221

   Promoter

   T7

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GB1-His-Tev-ALIX-V (348-702)

    Plasmid

    #189819

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His-Tev-ALIX-V (348-702).

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    GB1-His-Tev-ALIX-V (348-702) (PDCD6IP Human)
    

    Use

    Tags

    GB1-His-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. OTUB1/pRetroX-IRES-DsRed#10

    Plasmid

    #180279

    Purpose

    Plasmid encoding the human protease OTUB1 without a tag. Polycistronic red fluorescent retroviral vector (unmodified from TAKARA BIO).

    Depositor

    Andre Catic

    Article

    Moree et al Cancers (Basel). 2022 Feb 4;14(3). pii:

    Insert

    human Otubain-1 (OTUB1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Ad-HisFlag-USP19ER

    Plasmid

    #155244

    Purpose

    for generating adenoviruses expressing the ER isoform of rat USP19

    Depositor

    Simon Wing

    Article

    Wiles et al Mol Biol Cell. 2015 Mar 1;26(5):913-23.

    Insert

    USP19 (ER isoform) (Usp19 Rat)
    

    Use

    Adenoviral

    Tags

    6XHis-3XFlag

    Expression

    Mutation

    H118R- please see dep. comments

    Promoter

    CMV

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSG5HA-MAGE-112-429

    Plasmid

    #89131

    Purpose

    mammalian expression of N-terminal HA-tagged human MAGE-A11 amino acid residues 112-429

    Depositor

    Elizabeth Wilson

    Article

    Bai et al Mol Cell Biol. 2008 Mar;28(6):1947-63. d

    Insert

    MAGE-A11 (MAGEA11 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    contains aa 112-429

    Promoter

    SV40

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSG5-MAGE-TA360

    Plasmid

    #89141

    Purpose

    mammalian expression of full-length human MAGE-A11 amino acid residues 2-429 with TA360 phosphorylation site mutated

    Depositor

    Elizabeth Wilson

    Article

    Bai et al Mol Cell Biol. 2008 Mar;28(6):1947-63. d

    Insert

    MAGE-A11 (MAGEA11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T360A

    Promoter

    SV40

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA5 Flag-NFRKB delta101

    Plasmid

    #19790

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

    Insert

    NFRKB (NFRKB Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    deleted N-terminal 1-101 amino acids

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA5-Flag-Adrm1

    Plasmid

    #19417

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

    Insert

    adhesion regulating molecule 1 (ADRM1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pOPINB-OTULIN (full-length, aa 1-352)

    Plasmid

    #61464

    Purpose

    Expresses human OTULIN (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Keusekotten et al Cell. 2013 Jun 6;153(6):1312-26. doi: 10

    Insert

    OTULIN (OTULIN Human)
    

    Use

    Tags

    His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-HA-CYLD

    Plasmid

    #22544

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    CYLD (CYLD Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLAG-dCas13b-ADAR2DD

    Plasmid

    #190913

    Purpose

    Addgene #103871 with 3x FLAG on N-terminal of dCas13

    Depositor

    Timothy Jarome

    Article

    Farrell et al Mol Psychiatry. 2023 May 17. doi: 10.103

    Insert

    3X FLAG
    

    Use

    CRISPR

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTXB1-H2B(1-114)_wt

    Plasmid

    #118252

    Purpose

    Bacterial expression plasmid encoding X. laevis H2B residues 1-114 as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa

    Depositor

    Cynthia Wolberger

    Article

    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

    Insert

    xlH2B(1-114)
    

    Use

    Tags

    Mxe-GyrA intein fused to chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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