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pCAGGS-TAL-NC2

Plasmid

#43856

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

TALEN-FokI nuclease backbone

Tags

Flag and FokI nuclease

Expression

Mammalian

Promoter

CAG

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pTE-0X38-GCT1

Plasmid

#27053

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-gfp-ssrAec

Expression

Bacterial

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pDONR223-DGKK

Plasmid

#23487

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DGKK (DGKK Human)

Use

Gateway Cloning

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
hSyn-PostSynBar-hSyn-PostRNA short

Plasmid

#247716

Purpose

SynBar protein and RNA construct used in HEK and neuron culture experiment.

Depositor

Boxuan Zhao

Article

Chen et al Nat Methods. 2026 Mar 12. doi: 10.1038/s

Insert

PostSynBar and PostRNA short

Use

AAV

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pMUT2-pTcpP-mCherry

Plasmid

#192861

Purpose

pMUT2 based vector for sensing human primary bile acids in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

TcpP-CadC fusion protein and cognate promoter inducible by human primary bile acids

Expression

Bacterial

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pETM41_KPNB1_HEAT

Plasmid

#178494

Purpose

Bacterial expression of human domain with His-tag and MBP tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

KPNB1_HEAT (KPNB1 Human, Synthetic)

Tags

His-MBP

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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IBMc396

Plasmid

#161954

Purpose

Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHH

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T

Expression

Bacterial

Promoter

PBAD

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc090

Plasmid

#161937

Purpose

Carries engineered transposon for IBM

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pTHSSd_1V-WTR1R2-BbsI(G)-kanR-lacZalpha-SapI-WTR1R2

Expression

Bacterial

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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STAGR_SAMScaffold_hH1

Plasmid

#102847

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

STAgR Insert SAM_hH1

Use

Pcr template for stagr inserts

Promoter

U6

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pSR357.24

Plasmid

#125109

Purpose

Expression of narL(1-159aa)-ydfI(153-213aa) chimera under PLtetO-1, output Promoter PydfJ115

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL(REC)-ydfI(DBD)159
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSR357.10

Plasmid

#125106

Purpose

Expression of narL(1-131aa)-ydfI(126-213aa) chimera under PLtetO-1, output Promoter PydfJ115

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL(REC)-ydfI(DBD)131
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_ASCL3_P2A_Hygro_Barcode

Plasmid

#120428

Purpose

Barcoded lentiviral vector to express ASCL3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ASCL3 (ASCL3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-RFP-miR-223

Plasmid

#97148

Purpose

miR-223 expression

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

miR-223

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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MAC_C_ARP2

Plasmid

#111658

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

ARP2 (ACTR2 Human)

Expression

Mammalian

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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MAC_N_BET1

Plasmid

#111683

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

BET1 (BET1 Human)

Expression

Mammalian

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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Hox A11a

Plasmid

#36462

Depositor

Robert Ho

Article

Ahn et al Dev Biol. 2008 Oct 1;322(1):220-33. Epub

Insert

hoxa11a (hoxa11a Zebrafish)

Use

Riboprobe synthesis

Promoter

T7

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
pPS1889

Plasmid

#1361

Depositor

Pamela Silver

Article

Damelin et al Mol Cell 2000 Jan;5(1):133-40.

Insert

NLS-ECFP-EYFP

Expression

Yeast

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pBBR1-P2-SpCas9

Plasmid

#232354

Purpose

The plasmid pBBR1-P2-SpCas9 employs pBBR1MCS-2 as the vector, with Cas9 protein derived from S. pyogenes and the stationary phase promoter P2 sourced from S. marcescens HBQA7.

Depositor

Yujie Cai

Article

Gou et al Biotechnol Bioeng. 2025 May;122(5):1233-

Inserts

Cas9
sacB

Use

CRISPR

Promoter

promoter P2 from S. marcescens HBQA7

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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MAC-ACE2

Plasmid

#158381

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

ACE2 (ACE2 Human)

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEF-DEST51 GFP-PNRC1

Plasmid

#123295

Purpose

Mammalian expression of PNRC1 N-GFP

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

PNRC1 (PNRC1 Human)

Tags

GFP

Expression

Mammalian

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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