We narrowed to 2,858 results for: human cdnas

1. VN-alpha Synuclein

   Plasmid

   #89470

   Purpose

   expresses human alpha Synuclein fused to the N-terminal part of Venus

   Depositor

   Bradley Hyman, Tiago Outeiro

   Article

   Outeiro et al PLoS One. 2008 Apr 2;3(4):e1867. doi: 10

   Insert

   SNCA (SNCA Human)
   

   Use

   Tags

   N-terminal part of Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. hHS1-M7A

   Plasmid

   #159170

   Purpose

   Human codon-optimized cytosolic labile heme reporter

   Depositor

   Amit Reddi

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

   Insert

   hHS1-M7A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Mutated Met 7 of the cyt b562 module of hHS1 to A…

   Promoter

   CMV

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-Epac1 CAAX

   Plasmid

   #118312

   Purpose

   Expression of human Epac1

   Depositor

   Philip Stork

   Article

   Wang et al Mol Cell Biol. 2006 Mar;26(6):2130-45. d

   Insert

   Epac1 CAAX (RAPGEF3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   C-terminal 20aa of KiRas

   Promoter

   CMV

   Available since

   Nov. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. ADORA3-DuET

   Plasmid

   #213176

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   ADORA3 (ADORA3 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST40-2XFL-Src-S4-KD

   Plasmid

   #140297

   Purpose

   Expresses Flag-tagged human Src-S4-KD in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Use

   Tags

   2X Flag

   Expression

   Mammalian

   Mutation

   contains only the SH4 and KD regions

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. hCEP120-Myc

   Plasmid

   #50381

   Purpose

   Tet-On inducible expression of Myc-tagged hCEP120

   Depositor

   Tang Tang

   Article

   Lin et al J Cell Biol. 2013 Jul 22;202(2):211-9. d

   Insert

   Human CEP120 (CEP120 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   This hCEP120 contains 4 SNPs (rs6876883, rs659544…

   Promoter

   CMV-2xTetO

   Available since

   July 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. CHRM1-DuET

   Plasmid

   #213207

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CHRM1 (CHRM1 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSS1 (hLPL-FLAG)

   Plasmid

   #102447

   Purpose

   Expresses FLAG-tagged human LPL in mammalian cells

   Depositor

   Brandon Davies

   Article

   Chi et al J Biol Chem. 2015 May 8;290(19):11865-77

   Insert

   Lipoprotein lipase (LPL Human)
   

   Use

   Tags

   6x His and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-Epac1 SAAX

   Plasmid

   #118313

   Purpose

   Expression of human Epac1

   Depositor

   Philip Stork

   Article

   Wang et al Mol Cell Biol. 2006 Mar;26(6):2130-45. d

   Insert

   Epac1 SAAX (RAPGEF3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHS15 (hANGPTL3-Strep)

    Plasmid

    #109112

    Purpose

    Expresses StrepII-tagged human ANGPTL3 in mammalian cells

    Depositor

    Brandon Davies

    Article

    Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

    Insert

    ANGPTL3 (ANGPTL3 Human)
    

    Use

    Tags

    StrepII

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-NFIB-T2A-miRFP670

    Plasmid

    #187222

    Purpose

    Expresses human NFIB and miRFP670 via T2A linker under control of a CMV promoter.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    Nuclear Factor I B (NFIB Human)
    

    Use

    Tags

    Inserted T2A-miRFP670 at 3' terminal of MCS.

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST40-2XFL-Src-KD

    Plasmid

    #140313

    Purpose

    Expresses Flag-tagged human Src-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    Contains only the KD region

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTE3646

    Plasmid

    #137714

    Purpose

    Expresses LbCas12a RVRR variant in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    LbCas12a-RVRR
    

    Use

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    G532R, K538V, Y542R, K595R

    Promoter

    CMV

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. LIC-Z

    Plasmid

    #153543

    Purpose

    perform light induced optogenetic clustering of TCR ζ-Chain as described in the associated publication

    Depositor

    Katharina Gaus

    Article

    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

    Insert

    human TCR ζ-Chain (CD247 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    CMV

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSBbiRP Hu Full Length CD142 (Tissue Factor; F3)

    Plasmid

    #183254

    Purpose

    Expression of full length human tissue factor (CD142; F3) in a Sleeping Beauty transposon vector

    Depositor

    Alexander McLellan

    Article

    Muhsin-Sharafaldine et al Oncotarget. 2016 Aug 30;7(35):56279-5629

    Insert

    Tissue factor (F3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-Braf R188L

    Plasmid

    #118330

    Purpose

    Expression of human Braf

    Depositor

    Philip Stork

    Article

    Li et al J Biol Chem. 2013 Sep 20;288(38):27646-5

    Insert

    Braf R188L (BRAF Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    R188L

    Promoter

    CMV

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST40-2XHA-Src-KD

    Plasmid

    #140312

    Purpose

    Expresses HA-tagged human Src-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    Contains only the KD region

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEST40-2XHA-Src-S4-UD

    Plasmid

    #140304

    Purpose

    Expresses HA-tagged human Src-S4-UD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    contains only the SH4 and UD regions

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDEST40-2XHA-Src-S4-KD

    Plasmid

    #140296

    Purpose

    Expresses HA-tagged human Src-S4-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    contains only the SH4 and KD regions

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. hMEF2A K403R

    Plasmid

    #118355

    Purpose

    This plasmid expresses human MEF2A K403R mutant. This construct cannot undergo sumoylation on K403.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hMEF2A (MEF2A Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K403R

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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