We narrowed to 41,013 results for: Eras

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pD649-HAsp-SIRPD-COMP5AP-AviTag-9xHis

Plasmid

#157128

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SIRPD (SIRPD Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pTT3-SP-6xHis-TIE1(FL)-FLAG

Plasmid

#157621

Purpose

Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

TIE1 (TIE1 Human)

Tags

6xHis and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pClgn1.1_Dcst1-3xHA

Plasmid

#183540

Purpose

Express Dcst1-3xHA under the mouse Clgn promoter.

Depositor

Masahito Ikawa

Article

Noda et al Commun Biol. 2022 Apr 7;5(1):332. doi: 1

Insert

DC-STAMP domain containing 1 (Dcst1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pClgn1.1_Dcst2-3xHA

Plasmid

#183541

Purpose

Express Dcst2-3xHA under the mouse Clgn promoter.

Depositor

Masahito Ikawa

Article

Noda et al Commun Biol. 2022 Apr 7;5(1):332. doi: 1

Insert

DC-STAMP domain containing 2 (Dcst2 Mouse)

Tags

HA

Expression

Mammalian

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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MSNA-c028

Plasmid

#175470

Purpose

Protein expression in bacterial cells. FERM domain, M1-E346. Contains H288A point mutation that reduces binding to CD44.

Depositor

Opher Gileadi

Article

Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

Insert

MSN (MSN Human)

Tags

His6-TEV

Expression

Bacterial

Mutation

H288A

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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MSNA-c021

Plasmid

#175468

Purpose

Protein expression in bacterial cells. FERM domain, M1-E346. Contains L281R point mutation that reduces binding to CD44.

Depositor

Opher Gileadi

Article

Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

Insert

MSN (MSN Human)

Tags

His6-TEV

Expression

Bacterial

Mutation

L281R

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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MSNA-c001

Plasmid

#175466

Purpose

Protein expression in bacterial cells. FERM domain, M1-E346. Used for crystallography (PDB:6TXQ).

Depositor

Opher Gileadi

Article

Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

Insert

MSN (MSN Human)

Tags

His6-TEV

Expression

Bacterial

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-NQO1(Y128F)

Plasmid

#177141

Purpose

Bacterial vector for expression of an N-terminal GST fusion of NQO1(Y128F) with a TEV protease site located between the GST tag and NQO1(Y128F)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

NQO1 (NQO1 Human)

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pmirGLO-3'UTR-IL22

Plasmid

#153070

Purpose

Human IL-22 3'UTR region cloned downstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

3' UTR region of IL-22 gene (IL22 Human)

Use

Luciferase

Expression

Mammalian

Promoter

PGK

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (G112P)

Plasmid

#135496

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (G112P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

G112P

Promoter

CMV

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (L156P)

Plasmid

#135497

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (L156P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

L156P

Promoter

CMV

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-H2-Dd-VN (Y84C/C121S/A139C)

Plasmid

#135501

Purpose

Mammalian expression of VN-fused and myc-tagged H2-Dd (Y84C/C121S/A139C mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

H2-Dd (H2-D1 Mouse)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Y84C/C121S/A139C

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (I52P)

Plasmid

#135486

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (I52P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

I52P

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (Y59P)

Plasmid

#135492

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (Y59P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Y59P

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (D61P)

Plasmid

#135493

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (D61P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

D61P

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (V67P)

Plasmid

#135494

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (V67P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

V67P

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (Y84P)

Plasmid

#135495

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (Y84P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Y84P

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (E166P)

Plasmid

#135498

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (E166P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

E166P

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-KAT14-547-782

Plasmid

#118376

Purpose

Gateway compatible donor vector with KAT14-547-782 truncation.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

KAT14 (KAT14 Human)

Use

Donor-plasmid

Mutation

Truncation (aa 547-782)

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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Gal4-TET1-878-911-I894A(pL84)

Plasmid

#124404

Purpose

Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with I894A mutation.

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

Gal4-TET1-878-911(I894A mutation) (TET1 Human)

Tags

gal4 dna binding domain

Expression

Mammalian

Mutation

I894A

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Gal4-TET1-878-911-T898A(pL84)

Plasmid

#124403

Purpose

Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with T898A mutation.

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

Gal4-TET1-878-911(T898A mutation) (TET1 Human)

Tags

gal4 dna binding domain

Expression

Mammalian

Mutation

T898A

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Gal4-TET1-878-911-L900A(pL84)

Plasmid

#124402

Purpose

Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L900A mutation.

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

Gal4-TET1-878-911(L900A mutation) (TET1 Human)

Tags

gal4 dna binding domain

Expression

Mammalian

Mutation

L900A

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Gal4-TET1-878-911-L897A(pL84)

Plasmid

#124401

Purpose

Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L897A mutation.

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

Gal4-TET1-878-911(L897A mutation) (TET1 Human)

Tags

gal4 dna binding domain

Expression

Mammalian

Mutation

L897A

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-KAT14-1-546

Plasmid

#118377

Purpose

Gateway compatible donor vector with KAT14-1-546 truncation.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

KAT14 (KAT14 Human)

Use

Donor-plasmid

Mutation

Truncation (aa 1-546)

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCIneoRL-Notch1 3'UTR-mir200c-mut

Plasmid

#84596

Purpose

Renilla Luciferase reporter assay for NOTCH1 3'UTR with mutations on miR-200c binding site

Depositor

Yanan Yang

Article

Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

Insert

NOTCH1 3'UTR (NOTCH1 Human)

Use

Luciferase

Tags

Renilla Luciferase

Mutation

Mutation on miR-200c binding site on NOTCH1 3…

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only
pCIneoRL-LFNG 3'UTR-mir200c-mut

Plasmid

#84594

Purpose

Renilla Luciferase reporter assay for LFNG 3'UTR with mutations on miR-200c binding site

Depositor

Yanan Yang

Article

Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

Insert

LFNG 3'UTR (LFNG Human)

Use

Luciferase

Tags

Renilla Luciferase

Mutation

Mutation on miR-200c binding site on LFNG 3'…

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only
KLHL31 pgk (1088)

Plasmid

#62031

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

KLHL31 (Klhl31 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pZS1[GalS-L,RbsR-L]

Plasmid

#60763

Purpose

Contains PIq driving expression GalS-L, and PIq driving expression of RbsR-L.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

GalS-L
RbsR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with GalS L…

Available Since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[GalS-L,FruR-L]

Plasmid

#60764

Purpose

Contains PIq driving expression GalS-L, and PIq driving expression of FruR-L.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

GalS-L
FruR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with FruR L…

Available Since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[GalS-L,TreR-L]

Plasmid

#60765

Purpose

Contains PIq driving expression GalS-L, and PI driving expression of TreR-L.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

GalS-L
TreR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with GalS L…

Available Since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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