We narrowed to 148,810 results for: ins;

1. pRetroX-TRE3G-IRSp53

   Plasmid

   #221317

   Purpose

   Dox-inducible expression of IRSp53 in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   IRSp53 (Baiap2 Mouse)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEN84 - CTCF-AID[71-114]-eGFP-FRT-Puro-FRT targeting construct

   Plasmid

   #86230

   Purpose

   Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using puromycine selection. Auxin-inducible degron system.

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   AID[71-114]-eGFP
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCV-MAVS

   Plasmid

   #221313

   Purpose

   Expression of MAVS in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   MAVS (Mavs Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-nef-CIAO2-Flp

   Plasmid

   #149296

   Purpose

   Cre-dependent expression of Flp recombinase.

   Depositor

   Edward Callaway

   Article

   Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

   Insert

   Flp recombinase (flp Budding Yeast)
   

   Use

   AAV

   Mutation

   Derived from the mouse codon-optomized FlpO. The…

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-nef-CIAO-Flp

   Plasmid

   #149294

   Purpose

   Cre-dependent expression of Flp recombinase.

   Depositor

   Edward Callaway

   Article

   Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

   Insert

   Flp recombinase (flp Budding Yeast)
   

   Use

   AAV

   Mutation

   Derived from the mouse codon-optomized FlpO. The…

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB PUb-integrase

   Plasmid

   #183966

   Purpose

   piggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent marker

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

   Insert

   PhiC31 integrase
   

   Use

   Piggybac insect transgenesis destination vector

   Promoter

   Aedes aegypti Polyubiquitin

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabepuro3-p16Flag

   Plasmid

   #24934

   Depositor

   Lynda Chin

   Article

   Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

   Insert

   p16 (CDKN2A Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   July 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSP64-hKCNQ1

   Plasmid

   #53048

   Purpose

   Expresses alpha subunit for hKCNQ1 channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

   Insert

   Potassium voltage-gated channel subfamily KQT member 1 (KCNQ1 Human)
   

   Use

   Xenopus oocyte expression

   Promoter

   SP6

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. DRH323: pAAV_hSyn-QuasAr2

   Plasmid

   #107705

   Purpose

   in vivo voltage imaging

   Depositor

   Adam Cohen

   Article

   Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

   Insert

   QuasAr2
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-delta133HtrA-EGFP

    Plasmid

    #14124

    Depositor

    L. Miguel Martins

    Article

    Martins et al J Biol Chem. 2002 Jan 4. 277(1):439-44.

    Insert

    HtrA2 (HTRA2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of the first 133 amino-acids

    Available Since

    March 7, 2007

    Availability

    Academic Institutions and Nonprofits only

11. NKP90 RFP (787)

    Plasmid

    #62001

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    NKP90 (TMEM126A Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    pgk

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

12. CRY2olig-mCh-Nck

    Plasmid

    #60034

    Purpose

    Expresses a fusion containing CRY2olig (PHR, E490G) with mCherry and 3 SH3 domains of Nck

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

    Insert

    CRY2-mCh-NCK (CRY2 Human, Mustard Weed)
    

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    M354I, E490G CRY2

    Promoter

    CMV

    Available Since

    Nov. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHS1950

    Plasmid

    #205983

    Purpose

    Ga0116245_10002768 T7/LacO-Cas6 + TwinStrep-Cas14 + Cas7 + His14-Cas5::pJ23119-DR-Fn spacer-DR

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Inserts

    Ga0116245_10002768 candidate type VII proteins
    Cas14
    Cas5
    CRISPR array
    

    Tags

    14xHis and TwinStrep-tag

    Expression

    Bacterial

    Promoter

    T7 (proteins), pJ23119 (crRNA)

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. LADL Anchor

    Plasmid

    #127664

    Purpose

    Encodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoter

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    dCas9-CIBN-2A-Puro
    

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 (D10A,H840A) mutant

    Promoter

    EF1a

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTriex-mVenus-PI-RhoA(Q63L)

    Plasmid

    #86978

    Purpose

    PI(DM): Photo-activatable/light sensitive

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    RhoA (RHOA Human)
    

    Tags

    6xHis and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Q63L

    Promoter

    CMV

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET30a-SARS-CoV2-NP-Nt(47-173)

    Plasmid

    #165091

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173) (N SARS-CoV2)
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pBabepuro3-p18Flag

    Plasmid

    #24933

    Depositor

    Lynda Chin

    Article

    Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

    Insert

    P18 (CDKN2C Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    July 13, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3-KLF3

    Plasmid

    #49100

    Purpose

    Expresses KLF3 in mammalian cells

    Depositor

    Merlin Crossley

    Article

    Bell-Anderson et al Diabetes. 2013 Aug;62(8):2728-37. doi: 1

    Insert

    Krüppel-Like Factor 3(KLF3)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

19. Igfbp1-promoter/pGL3

    Plasmid

    #12146

    Depositor

    Domenico Accili

    Article

    Nakae et al EMBO J. 2000 Mar 1. 19(5):989-96.

    Insert

    IGFBP1 promoter (Igfbp1 Rat)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Contains the canonical insulin response sequence …

    Available Since

    Aug. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pJW2088

    Plasmid

    #154336

    Purpose

    Crispr repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::GFP::3xFLAG::10aa linker
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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