We narrowed to 6,748 results for: tac

1. GFP-Rab7A

   Plasmid

   #61803

   Purpose

   Human Rab7A fluorescently tagged with GFP on the N-terminus for mammalian expression

   Depositor

   Gia Voeltz

   Article

   Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

   Insert

   Rab7A (RAB7A Human)
   

   Use

   Tags

   2x HA and GFP

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-Dcp1b

   Plasmid

   #153976

   Purpose

   Fluorescent protein fused to mRNA decapping enzyme 1b

   Depositor

   Gia Voeltz

   Article

   Lee et al Science. 2020 Jan 31;367(6477). pii: 367

   Insert

   mRNA recapping enzybe 1b (DCP1B Human)
   

   Use

   Tags

   pAcGFP-C1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCh-alpha-tubulin

   Plasmid

   #49149

   Purpose

   expression of alpha-tubulin fused to mCherry. Used for visualization of microtubules.

   Depositor

   Gia Voeltz

   Article

   Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

   Insert

   alpha tubulin (TUBA1B Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. bdSUMO-GFP-HiBit

   Plasmid

   #170989

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-muGFP-HiBiT
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSmac-GFP

   Plasmid

   #40881

   Depositor

   Douglas Green

   Article

   Tait et al Dev Cell. 2010 May 18;18(5):802-13.

   Insert

   Smac-GFP (DIABLO Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

6. PC3-709-33-10_GAL4-FKBP12(F36V)

   Plasmid

   #200438

   Purpose

   Expresses the GAL4 DNA Binding domain linked to the FKBP12(F36V) target protein

   Depositor

   Xue Gao

   Article

   Ma et al J Am Chem Soc. 2023 Jan 25;145(3):1593-1

   Insert

   GAL4-FKBP12(F36V)
   

   Use

   Tags

   GAL4, FKBP12(F36V)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.hSyn.flex.H2B.GFP

   Plasmid

   #170368

   Purpose

   Cre-inducible AAV vector expressing nuclear GFP.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

   Insert

   flex H2B GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human Synapsin

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiCRISPR_zeo_sgNT135

   Plasmid

   #183181

   Purpose

   pLentiCRISPR that is selectable with zeocin with a non-targeting sgRNA

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   NT135
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTriEx4-Rac1-2G

   Plasmid

   #66110

   Purpose

   Second generation Rac1 FRET biosensor

   Depositor

   Olivier Pertz

   Article

   Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

   Insert

   Second generation Rac1 FRET biosensor
   

   Use

   Tags

   His-Myc

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiCRISPR-EGFP

    Plasmid

    #75159

    Purpose

    Derived from LentiCRISPR v1 but expresses EGFP instead of puromycin resistance

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. OMMGFP 1-10

    Plasmid

    #164119

    Purpose

    Expresses the non-fluorescent GFP1-10 moiety on the cytosolic face of the OMM

    Depositor

    Marisa Brini, Tito Calì

    Article

    Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

    Insert

    OMMGFP 1-10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-shRNA2-zsGreen-PGK-puro_scramble shRNA

    Plasmid

    #197991

    Purpose

    For the stable knockdown experiments using shRNA, scramble sequence was inserted as the control.

    Depositor

    Bo Wang

    Article

    Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

    Insert

    scramble shRNA (control)
    

    Use

    Lentiviral

    Tags

    zsGreen1

    Expression

    Mammalian

    Mutation

    15q11.2 deletion

    Promoter

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. VPS13A^Halo

    Plasmid

    #118759

    Purpose

    VPS13A internally tagged with Halo at residue 1372.

    Depositor

    Pietro De Camilli

    Article

    Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

    Insert

    VPS13A (VPS13A Human)
    

    Use

    Tags

    Halo tag inserted at position 1372

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pc3.1-CAGGS-ASAP3-mCyRFP3

    Plasmid

    #193323

    Purpose

    A red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3

    Depositor

    Michael Lin

    Article

    Kim et al Sci Rep. 2022 Mar 7;12(1):3678. doi: 10.

    Insert

    ASAP3-R3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    caggs

    Available since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

15. mRFP-FKBP12

    Plasmid

    #67514

    Purpose

    FKBP12 only

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

    Insert

    FKBP12 (FKBP1A Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRetroX-PTuner DD-linker-M.EcoGII

    Plasmid

    #122082

    Purpose

    Express M.EcoGII with detabilization domain - inducible

    Depositor

    Laure Crabbe

    Article

    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

    Insert

    DD-linker-M.EcoGII
    

    Use

    Retroviral

    Tags

    V5 tag on Nter of M.EcoGII

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTC391

    Plasmid

    #91220

    Purpose

    protoplast vector expressing gRNA23 targeting tomato ANT1 (with TREX2 expression)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    gRNA targeting tomato ANT1
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-FLAG-SBP-STAG2 (WT)

    Plasmid

    #73963

    Purpose

    STAG2 cDNA (CCDS43990) wild type

    Depositor

    Todd Waldman

    Article

    Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

    Insert

    STAG2 (STAG2 Human)
    

    Use

    Tags

    FLAG-SBP

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

19. LVDP 2P.2

    Plasmid

    #231904

    Purpose

    For the production of SARS-CoV-2 virus-like particles (VLPs) in the '2-plasmid' system and packaging firefly luciferase mRNA into the VLPs

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

    Insert

    nucleocapsid protein-T2A-membrane protein-IRES-envelope protein (N, E, M SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    R203M in N protein

    Promoter

    CMV, EF-1a

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLentiCRISPR-RFP657

    Plasmid

    #75162

    Purpose

    Derived from LentiCRISPR v1 but expresses RFP657 instead of puromycin resistance

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    RFP657

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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