We narrowed to 505 results for: SPY

1. pDEST14-Avitag-SpyCatcher002

   Plasmid

   #102828

   Purpose

   Bacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylation

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   Avitag-SpyCatcher002
   

   Use

   Tags

   Avitag, GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-SpyCatcher-mi3

   Plasmid

   #112255

   Purpose

   Expresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

   Depositor

   Mark Howarth

   Article

   Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

   Insert

   SpyCatcher-mi3
   

   Use

   Tags

   C-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Dead Streptavidin-SpyCatcher

   Plasmid

   #59547

   Purpose

   pET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers

   Depositor

   Mark Howarth

   Article

   Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

   Insert

   Dead-dloop-Streptavidin-SpyCatcher
   

   Use

   Tags

   N-terminally shortened SpyCatcher

   Expression

   Bacterial

   Mutation

   PolyAsp in L3/4 loop for ion-exchange

   Promoter

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pHR-Spy-CD86

   Plasmid

   #223598

   Purpose

   Lentiviral expression of human CD86 with SpyTag003 in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   CD86 (CD86 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   Spytag003

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET11b-SpyCatcher-HA2

   Plasmid

   #165991

   Purpose

   SpyCatcher-fused influenza HA ministem with removable HisTag

   Depositor

   Vesa Hytönen

   Article

   Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

   Insert

   SpyCatcher-HA2
   

   Use

   Tags

   Foldon, HisTag, and SpyCatcher

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Spy CCR5-pegRNA

   Plasmid

   #169856

   Purpose

   SpyCas9-pegRNA for CCR5

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   Spy CCR5-pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 promoter

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Dead Streptavidin-SpyTag

   Plasmid

   #59548

   Purpose

   pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers

   Depositor

   Mark Howarth

   Article

   Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

   Insert

   Dead Streptavidin-SpyTag
   

   Use

   Tags

   SpyTag

   Expression

   Bacterial

   Mutation

   The construct lacks final K of SpyTag

   Promoter

   T7

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pET28a-SUMO-SpyTag003

   Plasmid

   #216282

   Purpose

   Expresses SUMO with a C-terminal SpyTag003 in bacterial cells

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SUMO-SpyTag003
   

   Use

   Tags

   His6 and SpyTag003

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET11b-SpyCatcher-M2e

   Plasmid

   #165990

   Purpose

   SpyCatcher-fused influenza M2 ectodomain with removable HisTag

   Depositor

   Vesa Hytönen

   Article

   Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

   Insert

   SpyCatcher-M2e
   

   Use

   Tags

   HisTag and SpyCatcher

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Spy EMX1-pegRNA

    Plasmid

    #169855

    Purpose

    SpyCas9-pegRNA for EMX1

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    Spy EMX1-pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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