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pILGFP4QE2Crimson

Plasmid

#218280

Purpose

Introduction of a bicistronic expression cassette of yEGFP and E2Crimson under the control of the SkGAL2 promoter

Depositor

Claudia Vickers

Article

Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

Insert

pURA3>KlURA3>tKlURA3-pSkGAL2>yEGFP>ERBV1.2A>E2Crimson>tURA3

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pv7_MBD1_MBD_noEGFP

Plasmid

#179406

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MBD1_MBD

Use

Tags

AviTag

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pv7_CBX1_2xChromo_noEGFP

Plasmid

#179395

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo_noEGFP

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX1_2xChromo_noEGFP

Use

Tags

AviTag

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha2-p35S:HR1bR181/185A -GFP-gRHD6-UTR-Ter

Plasmid

#211847

Purpose

Plant transient expression cassette for RHD6 fused with GFP and a mutant form of the HR1b degron

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

HR1b

Use

Synthetic Biology

Tags

GFP

Expression

Plant

Mutation

R181A R185A

Promoter

35S

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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LV TRE-GFP-TMD-DLL1-Nbox-mutant

Plasmid

#194937

Purpose

LV construct encoding a mutated GFP-TMD-DLL1 ligand containing an ablated Nbox site under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

eGFP-(PDGFR)TMD-rDLL1 ICD-Nbox-AlaX3-mutant

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBBK42 pHomL-RPL18Bp-GFPdropout-Link-3HA-SSA1t-HomR (AmpR)

Plasmid

#179026

Purpose

Parent vector for markerless yeast integration cassettes with C-terminal 3xHemagglutinin-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK41 pHomL-TEF2p-GFPdropout-Link-3HA-SSA1t-HomR (AmpR)

Plasmid

#179025

Purpose

Parent vector for markerless yeast integration cassettes with C-terminal 3xHemagglutinin-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK40 pHomL-TDH3p-GFPdropout-Link-3HA-SSA1t-HomR (AmpR)

Plasmid

#179024

Purpose

Parent vector for markerless yeast integration cassettes with C-terminal 3xHemagglutinin-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK39 pHomL-RPL18Bp-3HA-Link-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179023

Purpose

Parent vector for markerless yeast integration cassettes with N-terminal 3xHemagglutinin-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK38 pHomL-TEF2p-3HA-Link-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179022

Purpose

Parent vector for markerless yeast integration cassettes with N-terminal 3xHemagglutinin-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK37 pHomL-TDH3p-3HA-Link-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179021

Purpose

Parent vector for markerless yeast integration cassettes with N-terminal 3xHemagglutinin-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-flex-GFP-4x6T

Plasmid

#196418

Purpose

Cre-dependent AAV expression of GFP preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Insert

GFP

Use

AAV and Cre/Lox; Astrocyte-selective

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9VPH-T2A-GFP-shP53

Plasmid

#102895

Purpose

EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

dCas9VPH-T2A-GFP-shP53

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pGEM-myr-eGFP-SV40pA-FKF

Plasmid

#119866

Purpose

Donor cassette containing myr-tagged eGFP and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

Depositor

Tatjana Sauka-Spengler

Article

Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

Insert

myr-tagged eGFP and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene

Use

Tags

2x myristoylation

Expression

Bacterial

Mutation

Promoter

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCALNL-eGFP-NT1-6-gix-6-NT1

Plasmid

#81204

Purpose

contains two target sites consisting of PAM-NT1-6bp-gix psuedo site-6bp-NT1-PAM flanking a kan/pA as previously described in the pCALNL-eGFP plasmid

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

gix-Neo-gix deletion cassette upstream of EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCBa

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-PuroR-T2A-eGFP

Plasmid

#234734

Purpose

Puromycin resistance/eGFP cassette in mammalian cells (transfection marker)

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

PuroR-T2A-eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pSH231-EF1-GFP-HYGRO

Plasmid

#115144

Purpose

Safe harbor site 231 knock-in vector with GFP-HygromycinR expression cassette

Depositor

Michael Phelps, Raymond Monnat

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG

Plasmid

#174891

Purpose

amber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG in sfGFP

Promoter

EF1

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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PB-tight-DDdCas9GFP-IRES-Neo

Plasmid

#102907

Purpose

PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

DDdCas9GFP-IRES-Neo

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A, H840A

Promoter

TRE-tight

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pT2/CMV-eGFP.PB/PTK

Plasmid

#170540

Purpose

Expresses the puromycin resistance gene fused to a thymidine kinase, upon excision of this cassette the reading frame of eGFP is restored

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Insert

Delta 5'-eGFP CMV-PuroTK- Delta 3'-eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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