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Showing: 361 - 383 of 383 results

  1. pJT055 AAVS1-BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #187324
    Purpose
    Donor to install a dual surface marker - fluorescence reporter into the AAVS1 safe harbor using HDR
    Depositor
    Michael Bassik, Lacra Bintu
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF1a
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    187324-plasmid-map-sequence-id-369800

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  3. pPL001-BBTK

    Plasmid
    #184751
    Purpose
    Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD. Harbors a backbone counterselectable TK cassette.
    Depositor
    Jef Boeke
    Article
    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118
    Insert
    pEF1a-eGFP-T2A-BSD-bGHpA
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    184751-plasmid-map-sequence-id-365380

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  9. pJT340 Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + MGA1+2 + RYBP-T2A-tagBFP-P2A-Blast

    Plasmid
    #187344
    Purpose
    dCas9 effector fusion for manipulating transcription
    Depositor
    Michael Bassik, Lacra Bintu
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    dCas9-3XFLAG-KRAB[WSR7EEE] + MGA1+2 + RYBP-T2A-tagBFP-P2A-Blast
    Use
    CRISPR and Lentiviral
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    KRAB WSR7EEE
    Promoter
    pEF1a
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    187344-plasmid-map-sequence-id-373294

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  11. pJT242 Lenti pEF-dCas9-3XFLAG-ZNF10 KRAB[WSR7EEE] HighExpr-T2A-tagBFP-P2A-Blast

    Plasmid
    #187327
    Purpose
    dCas9 effector fusion for manipulating transcription
    Depositor
    Michael Bassik, Lacra Bintu
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    dCas9-3XFLAG-ZNF10 KRAB[WSR7EEE] HighExpr-T2A-tagBFP-P2A-Blast
    Use
    CRISPR and Lentiviral
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    KRAB WSR7EEE
    Promoter
    pEF1a
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    187327-plasmid-map-sequence-id-373284

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  13. pJT341 Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + RYBP + MGA1+2-T2A-tagBFP-P2A-Blast

    Plasmid
    #195540
    Purpose
    dCas9 effector fusion for manipulating transcription
    Depositor
    Michael Bassik, Lacra Bintu
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + RYBP + MGA1+2-T2A-tagBFP-P2A-Blast
    Use
    CRISPR and Lentiviral
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    pEF1a
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    195540-plasmid-map-sequence-id-387166

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  22. pMV7 lenti pEF-rTetR(SE-G72P)-3XFLAG-CACO1(Zn-C2H2_12)-T2A-mCherry-BSD-WPRE

    Plasmid
    #188753
    Purpose
    Lentiviral backbone containing CACO1 Zn-C2H2_12 domain as a fusion with rTetR(SE-G72P)-3XFLAG
    Depositor
    Michael Bassik, Lacra Bintu
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    CACO1 Zn-C2H2_12 (CALCOCO1 Human)
    Use
    Lentiviral
    Tags
    rTetR(SE-G72P)-3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    pEF1a
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    188753-plasmid-map-sequence-id-371427

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  23. Human/mouse ELL2

    Plasmid
    #127254
    Purpose
    Expresses a human/mouse chimera that increases ELL2 expression in mammalian cells
    Depositor
    Christine Milcarek
    Article
    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi
    Insert
    N-terminal human/C-terminal mouse ELL2 (ELL2, Ell2 Mouse, Human)
    Use
    Other
    Tags
    His6X
    Expression
    Mammalian
    Mutation
    N terminal amino acids (1-287) are human, C termi…
    Promoter
    pEF1a
    Available since
    Aug. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    127254-plasmid-map-sequence-id-250057

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Showing: 361 - 383 of 383 results