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pFasBac+GFP-CAMSAP3 CC+CKK

Plasmid

#59041

Purpose

for baculovirus expression of mouse CAMSAP3 CC+CKK in Sf9 cells

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP3 CC+CKK domain (Camsap3 Mouse)

Tags

His and eGFP

Expression

Insect

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB DPP9S D808N

Plasmid

#169947

Purpose

Purification of catalytically-dead DPP9S from insect cells

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

HIS-TEV

Expression

Insect

Mutation

D808N

Promoter

polyhedrin

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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Pan-Neurofascin scFv [L11A/41]

Plasmid

#206722

Purpose

Mammalian Expression of Pan-Neurofascin scFV. Derived from hybridoma L11A/41 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Pan-Neurofascin (Rattus norvegicus) recombinant scFV (Nfasc Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIM5alpha(chimpanzee)

Plasmid

#79032

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(chimpanzee)

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

polyhedrin

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PIK3CA - Exon9 E545K (pFastBac)

Plasmid

#16640

Depositor

Bert Vogelstein

Article

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA E545K (PIK3CA Human)

Use

Baculoviral

Tags

His

Expression

Mammalian

Mutation

E545K

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
PIK3CA - Exon20 H1047R (pFastBac)

Plasmid

#16641

Depositor

Bert Vogelstein

Article

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA H1047R (PIK3CA Human)

Use

Baculoviral

Tags

His

Expression

Mammalian

Mutation

H1047R

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
pFastBac-CHD7 (787-1899)

Plasmid

#170145

Purpose

Expresses residues 787-1899 of CHD7 in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Tags

FLAG

Expression

Insect

Mutation

N-terminal truncation of residues 1-786; C-termin…

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (787-2138)

Plasmid

#170146

Purpose

Expresses residues 787-2138 of CHD7 in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Tags

FLAG

Expression

Insect

Mutation

N-terminal truncation of residues 1-786; C-termin…

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (936-2997)

Plasmid

#170147

Purpose

Expresses residues 936-2997 of CHD7 in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Tags

FLAG

Expression

Insect

Mutation

N-terminal truncation of residues 1-935

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-His-Flag-Tipin

Plasmid

#22890

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Tipin (TIPIN Human)

Tags

Flag- and His

Expression

Insect

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pFasBac+GFP-CAMSAP2 CC+CKK

Plasmid

#59039

Purpose

for baculovirus expression of CAMSAP2 CC+CKK in Sf9 cells

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP2 CC+CKK domains (CAMSAP2 Human)

Tags

His and eGFP

Expression

Insect

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pfast bac-flag Fbxl3

Plasmid

#51894

Purpose

Backbone plasmid is pfastBacHTa from invitrogen

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

Fbxl3 (Fbxl21 Mouse)

Tags

FLAG

Promoter

polyhedrin promoter

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pFastBac-His-Tipin-L195A

Plasmid

#23121

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

Insert

Tipin (TIPIN Human)

Tags

His

Expression

Insect

Mutation

Changed Leucine 195 to Alanine

Available Since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pFastBac1/CALR del52-His

Plasmid

#214795

Purpose

Baculovirus expression of human CALR del52-His

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR del52-His (CALR Human)

Tags

His

Expression

Insect

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac1/CALR ins5-His

Plasmid

#214796

Purpose

Baculovirus expression of human CALR ins5

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR ins5 (CALR Human)

Tags

His

Expression

Insect

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-FastFUCCI (JDW 1494)

Plasmid

#242592

Purpose

A CAGGS driven expression vector containing FastFUCCI to label cell cycle state.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac1-His-GFP-RAD54L2

Plasmid

#247924

Purpose

Insect expression construct for N-terminal His-GFP-tagged RAD54L2

Depositor

Junjie Chen, Huimin Zhang

Article

Zhang et al Sci Adv. 2023 Dec 8;9(49):eadi6681. doi:

Insert

RAD54L2 (RAD54L2 Human)

Tags

His-GFP

Expression

Insect

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-SYN1>Fas-EGFP:P2A:mNaChBac.

Plasmid

#242230

Purpose

Express Fas-EGFP and mNaChBac in neurons

Depositor

Qingchun Tong

Article

Li et al Cell Rep. 2025 Jun 19;44(7):115863. doi:

Inserts

Fas
EGFP
P2A
mNachBac

Use

AAV

Expression

Mammalian

Promoter

SYN

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac1-ScVip1-186-1107

Plasmid

#238316

Purpose

ScVip1-186-1107 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-186-1107

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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OMM-short-RspA-CFAST

Plasmid

#233594

Purpose

Expression of RspA-CFAST on the outer mitochondrial membrane

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

TOM70 fragment-RspA-CFAST (TOMM70 RspA-CFAST from Rheinheimera sp A13L and TOM70 fragment from Homo sapiens, Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac SOX 318-320A

Plasmid

#192060

Purpose

Expression vector for SOX (ORF37) 318-320A

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (ORF37)

Expression

Insect

Mutation

318-320A

Promoter

Polyhedrin

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-NEDD4L_W2-W3-W4-HECT (349)

Plasmid

#187721

Purpose

Express GST-tagged NEDD4L W2-W3-W4-HECT (349) in insect cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 349-975 (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Insect

Mutation

deletion of residues 1-348

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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Golgi-FAST-IRES-iRFP670

Plasmid

#216345

Purpose

For testing localization of pFAST and iRFP670 proteins to Golgi complex

Depositor

Alison Tebo

Insert

ST6Gal1_pFAST-IRES-Sys1_iRFP670

Tags

myc for FAST and iRF670 proteins

Expression

Mammalian

Mutation

n/a

Promoter

CMV

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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Peroxisomes-FAST-IRES-iRFP670

Plasmid

#216347

Purpose

For testing localization of pFAST and iRFP670 proteins to peroxisomes

Depositor

Alison Tebo

Insert

pFAST_SKL-IRES-iRFP670_PXMP2

Tags

myc for FAST protein only

Expression

Mammalian

Mutation

n/a

Promoter

CMV

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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Mito-FAST-IRES-iRFP670

Plasmid

#216346

Purpose

For testing localization of pFAST and iRFP670 proteins to mitochondria

Depositor

Alison Tebo

Insert

Cox8_pFAST-IRES-Tomm20_iRFP670

Tags

myc for FAST and iRF670 proteins

Expression

Mammalian

Mutation

n/a

Promoter

CMV

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-His6-E3(1)-CUbo-TwinStrep

Plasmid

#212712

Purpose

Expression of His6-E3(1)-CUbo-TwinStrep in insect cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Tags

6xHis and CUbo-TwinStrep

Expression

Bacterial

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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PMP34-mApple-NFAST(low)

Plasmid

#214418

Purpose

Peroxisome localization of low-affinity NFAST fused to PMP34-Apple

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspA-N (SLC25A17 )

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-837_NY-ESO-1_TCR_tFAS

Plasmid

#207500

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

tFAS, NY-ESO-1_TCR (FAS Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-Clover4-LUMABS-S1 RBD

Plasmid

#183491

Purpose

To express the Clover4-LUMABS-S1 RBD sensor for SARS-CoV-2 antibody dection.

Depositor

Jun Chu, Zongwen Jin

Article

Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

Insert

Clover4-LUMABS-S1 RBD

Expression

Insect

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2393-Tier1-PhCMV-p2a_fastFT

Plasmid

#169530

Purpose

Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven P2A-fused fluorescent timer, fast maturation time

Expression

Mammalian

Promoter

PhCMV

Available Since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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