We narrowed to 295 results for: 1303
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Plasmid#1303DepositorInsertHsp104 Y257W (HSP104 Budding Yeast)
UseTags10X His tagExpressionBacterialMutationChanged Tyr 257 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only
These results may also be relevant.
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meffRed chromoprotein
Plasmid#117836PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses meffRed chromoprotein in E. coliDepositorInsertpromoter, RBS, meffRed
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
amilGFP chromoprotein promoter-less
Plasmid#118006PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertRBS, amilGFP
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
tsPurple chromoprotein promoter-less
Plasmid#118007PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertRBS, tsPurple
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSico-shRaptor
Plasmid#81086Purposeconditional expression of an shRNA targeting murine RaptorDepositorInsertRaptor shRNA (Rptor Mouse)
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceSept. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28-mTDGa.0
Plasmid#81050Purposebacterial expression of murine TDGDepositorInsertTDG (Tdg Mouse)
UseTags6HISExpressionBacterialMutationPromoterT7Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTG-mTDG-K341R
Plasmid#52267PurposeBacterial expression of mouse TDG-K341R with C-terminal GST-tagDepositorInsertTDG (Tdg Mouse)
UseTagsGSTExpressionBacterialMutationK341R, SUMO acceptor site mutationPromoterT7Available sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
promoter J23110 (upstream of RBS, mRFP1)
Plasmid#117852PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertmedium-high promoter, RBS, mRFP1
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRA1SEGFPTcIfm
Plasmid#193777PurposeCassette 1: Expresses EGFP under the control of PR promoter, Cassette 2: Expresses frame-shifted CI under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selectionDepositorInsertEGFP and frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPR promoter and PLTetO-1Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTA1cI-T3
Plasmid#193789PurposeExpresses truncated version of lambda CI named T3 under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selectionDepositorInsertTruncated version of Lambda CI, named T3, where the first two codons are deleted
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTetO-1Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTG-mTDGa.0
Plasmid#81048Purposebacterial expression of murine TDGDepositorInsertTDG (Tdg Mouse)
UseTagsGSTExpressionBacterialMutationPromoterT7Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
hHS1-M7A
Plasmid#159170PurposeHuman codon-optimized cytosolic labile heme reporterDepositorInserthHS1-M7A
UseTagsExpressionMammalianMutationMutated Met 7 of the cyt b562 module of hHS1 to A…PromoterCMVAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
S142D drebrin-YFP
Plasmid#58336Purposemammalian expression of human drebrin-YFP phospho-mimetic mutantDepositorInsertdrebrin (DBN1 Human)
UseTagsEYFPExpressionMammalianMutationS142D, phospho-mimetic mutantPromoterCMVAvailable sinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
S142A drebrin-YFP
Plasmid#58335Purposemammalian expression of human drebrin-YFP phospho-dead mutantDepositorInsertdrebrin (DBN1 Human)
UseTagsEYFPExpressionMammalianMutationS142A, phospho-dead mutantPromoterCMVAvailable sinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
mApple-Vinculin-23
Plasmid#54961PurposeLocalization: Focal Adhesions, Excitation: 568, Emission: 592DepositorInsertVinculin (VCL Human)
UseTagsmAppleExpressionMammalianMutationV234L in Vinculin.PromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTCO2-mTDGi.0
Plasmid#149429PurposeminiTdg geneDepositorInsertTdg (Tdg Mouse)
UseCre/LoxTagsExpressionMammalianMutationSNPs of the Tdg gene from the OLA/129 strainPromoterTdg promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.JAG1-F1.GS.CAR-2G
Plasmid#194461PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-JAG1 CAR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-mTDGa.1
Plasmid#81051Purposebacterial expression of catalytically dead murine TDGDepositorInsertTDG (Tdg Mouse)
UseTags6HISExpressionBacterialMutationasparagine 151 changed to alaninePromoterT7Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.JAG1-B5.GS.CAR-2G
Plasmid#194463PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-JAG1 CAR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTG-mTDGa.1
Plasmid#81049Purposebacterial expression of catalytically dead murine TDGDepositorInsertTDG (Tdg Mouse)
UseTagsGSTExpressionBacterialMutationasparagine 151 changed to alaninePromoterT7Available sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
p2701-AGER-tdTomato
Plasmid#216470Purposedonor vector for targeting a tdTomato reporter to the human AGER locus at the endogenous ATG start siteDepositorInserttdTomato
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only