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TRPML1-G-GECO1.2-ERES
Plasmid#207144PurposeFusion of Ca2+ reporter and lysosomal Ca2+-permeable channel, with ER-export signalDepositorInsertMcoln1 (MCOLN1 Human)
UseTagsER-Export Signal (ERES) and G-GECO1.2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0093
Plasmid#143009PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZEB1 (ZEB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0090
Plasmid#142456PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZEB1 (ZEB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0091
Plasmid#142457PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZEB1 (ZEB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-SUMO-MproC145A
Plasmid#172653PurposeBacterial expression of SARS-CoV-2 Mpro C145A inactive mutant fused to SUMODepositorInsertSARS-CoV-2 Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTags6xHis Tag and SUMOExpressionBacterialMutationCatalytic Cysteine 145 mutated to AlaninePromoterT7Available sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF0092
Plasmid#142533PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZEB1 (ZEB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0089
Plasmid#142532PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZEB1 (ZEB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFv_Her2-BFP_RHL027
Plasmid#164823PurposeLentiviral vector for constitutive expression of HER2+TM(23-675)-BFPDepositorInsertExtracellular and transmembrane region of ERBB2 (ERBB2 Human)
UseLentiviralTagstagBFPExpressionMutationPromoterSFFVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCWXPGR-pTF-dnTCF4
Plasmid#114277PurposeTET-inducible expression of a dominant negative form of the TCF4 transcription factorDepositorInsertTCF4 (TCF4 Human)
UseLentiviralTagsExpressionMutationdeletion of AA 1 to 31PromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pLOC-MCOLN1
Plasmid#131583PurposeFor expression of human MCOLN1 in mammalian cells, GFP-taggedDepositorInsertMCOLN1 (MCOLN1 Human)
UseLentiviralTagsturboGFP tagExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-dnTCF4-ires-GFP
Plasmid#133378PurposePiggybac vector for constitutive expressionDepositorInsertdnTCF4 (TCF4 Human)
UseTags2x FlagExpressionMammalianMutationdeltaN31PromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
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NPR2 gRNA (BRDN0001147043)
Plasmid#77754Purpose3rd generation lentiviral gRNA plasmid targeting human NPR2DepositorInsertNPR2 (NPR2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-TRPML1(PHD)
Plasmid#221752PurposeStable expression of GFP-TRPML1(PHD) in mammalian cellsDepositorInsertTRPML1 (MCOLN1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationtandem TRPML1(PHD) (amino acids 1–69)PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
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