We narrowed to 4,801 results for: PIR

1. NaVAe1 Sp1 CTD pIRES

   Plasmid

   #128085

   Purpose

   Mammalian cell expression vector for (NaVAe1 1-243 NaVSp1 220-255) chimera bacterial sodium channel.

   Depositor

   Dan Minor

   Article

   Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

   Insert

   NaVAe1 and Sp1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)

   Plasmid

   #107939

   Purpose

   pie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.

   Depositor

   Heng-Chi Lee

   Article

   Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

   Insert

   mCherry(dpiRNA)::ANI-1(AHPH)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   pie-1

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIRES-H3.3-G34R-FLAG-BFP

   Plasmid

   #206140

   Purpose

   Expression of a FLAG tagged human histone mutant H3.3-G34R with BFP

   Depositor

   Maria Castro

   Insert

   H3.3 (H3-3A Human)
   

   Use

   Tags

   FLAG-eBFP

   Expression

   Mammalian

   Mutation

   G34R

   Promoter

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIRES_IBB-mCherry-IRES-HA-mAID-nanobody

   Plasmid

   #117720

   Purpose

   Expression of nuclear transfection marker IBB-mCherry and HA-tagged mAID-nanobody from one mRNA

   Depositor

   Joerg Mansfeld

   Article

   Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

   Inserts

   mAID-nanobody
   IBB-mCherry
   

   Use

   Tags

   importin-beta bining domain (IBB) and triple HA

   Expression

   Mammalian

   Mutation

   codon optimized mAID for murine expression

   Promoter

   CMV and None, IRES-driven

   Available since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. M78 pIRESpuro-CBP-2xTEV-StrepTag

   Plasmid

   #17132

   Depositor

   Randall Moon

   Article

   Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

   Type

   Empty backbone

   Use

   Tags

   2xTEV, CBP, and Streptavidin

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. CaM34(D93A/D129A)/pIRES2-eGFP

   Plasmid

   #111517

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   calmodulin [Xenopus laevis] (calm2 Frog)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D93A/D129A

   Promoter

   Available since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. CaM12(D20A/D56A)/pIRES2-eGFP

   Plasmid

   #111512

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   calmodulin [Xenopus laevis] (calm2 Frog)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D20A/D56A

   Promoter

   Available since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIRES-mRFP-NLS-PCNA (2514)

   Plasmid

   #61850

   Purpose

   cell cycle marker

   Depositor

   Jonathon Pines

   Insert

   PCNA (PCNA Human)
   

   Use

   Tags

   NLS and mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pMNK55 - piRNAi 21ur-1224 - cku-80

   Plasmid

   #159820

   Purpose

   piRNA-induced RNA interference (piRNAi) to bias double-strand repair toward homologous recombination

   Depositor

   Christian Froekjaer-Jensen

   Article

   Priyadarshini et al Nat Methods. 2022 Feb;19(2):187-194. doi

   Insert

   cku-80 piRNAi 21ur-1224
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMNK54 - piRNAi 21ur-1224 - him-5

    Plasmid

    #159818

    Purpose

    piRNA-induced RNA interference (piRNAi) to induce males in transgenic animals

    Depositor

    Christian Froekjaer-Jensen

    Article

    Priyadarshini et al Nat Methods. 2022 Feb;19(2):187-194. doi

    Insert

    him-5 piRNAi 21ur-1224
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMNK56 - piRNAi 21ur-1224 - him-8

    Plasmid

    #159819

    Purpose

    piRNA-induced RNA interference (piRNAi) to induce males in transgenic animals

    Depositor

    Christian Froekjaer-Jensen

    Article

    Priyadarshini et al Nat Methods. 2022 Feb;19(2):187-194. doi

    Insert

    him-8 piRNAi 21ur-1224
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. hPlekhm1-N/pIRES puro Glue (M1-A497)

    Plasmid

    #73454

    Purpose

    Express human N-terminal part (M1-A497) Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Use

    Tags

    CBP tag, Ha tag, and strep tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pIRES2-eGFP-Ofd1-Myc-S75F

    Plasmid

    #24580

    Depositor

    Jeremy Reiter

    Article

    Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

    Insert

    Ofd1 (Ofd1 Mouse)
    

    Use

    Tags

    Myc and eGFP

    Expression

    Mammalian

    Mutation

    serine 75 changed to phenylalanine (codon TCT cha…

    Promoter

    Available since

    June 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIRES2-eGFP-Ofd1-Myc-A80T

    Plasmid

    #24579

    Depositor

    Jeremy Reiter

    Article

    Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

    Insert

    Ofd1 (Ofd1 Mouse)
    

    Use

    Tags

    Myc and eGFP

    Expression

    Mammalian

    Mutation

    Alanine 80 changed to threonine (codon GCA to ACA)

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIRES2-eGFP-Ofd1-Myc-G139S

    Plasmid

    #24581

    Depositor

    Jeremy Reiter

    Article

    Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

    Insert

    Ofd1 (Ofd1 Mouse)
    

    Use

    Tags

    Myc and eGFP

    Expression

    Mammalian

    Mutation

    Glycine 139 changed to serine (codon GGT to AGT)

    Promoter

    Available since

    June 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pIRES2-eGFP-Ofd1-Myc-S437R

    Plasmid

    #24582

    Depositor

    Jeremy Reiter

    Article

    Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

    Insert

    Ofd1 (Ofd1 Mouse)
    

    Use

    Tags

    Myc and eGFP

    Expression

    Mammalian

    Mutation

    Serine 437 changed to Arginine (codon AGT to CGT)

    Promoter

    Available since

    June 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIRES2-eGFP:HA-mTREK-1(tandem S131C-wt)

    Plasmid

    #224777

    Purpose

    Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Tandem TREK-1 (S131C-wt) (Kcnk2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S131C

    Promoter

    CMV

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIRES2-eGFP:HA-mTREK-1(S131C/A286F)

    Plasmid

    #224775

    Purpose

    Mammalian expression vector for generating mouse TREK-1

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S131C / A286F

    Promoter

    CMV

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIRES2-eGFP:HA-mTREK-1(tandem wt-S131C)

    Plasmid

    #224778

    Purpose

    Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Tandem TREK-1 wt-S131C) (Kcnk2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S562C

    Promoter

    CMV

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIRHD-TetR-GAL80-Rho1-GAL4

    Plasmid

    #218274

    Purpose

    Introduction of low-temperature-inducible GAL system, LowTempGAL v2.2

    Depositor

    Christopher Howard

    Article

    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

    Insert

    gal80Arm1-pAgTEF1>KanMX4>tAgTEF1-pHAC1>UBI4>>DHFRP66L-TetR>NLS>TUP1>tABF1-pTEF2>Rho1C17R>>GAL4>tGAL4-pTEF1(TetO)>GAL80Arm2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    DHFR (P66L), Rho1 (C17R)

    Promoter

    Available since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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