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pBWB162

Plasmid

#140634

Purpose

pBAV1k-lacI-Trc-mCherry base, lacI repression vector for ADP1

Depositor

Keith Tyo

Article

Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-Tornado-split-nLuc

Plasmid

#212612

Purpose

For the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

Tornado-CVB3-split-nLuc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

C373T mutation in CVB3

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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EF06R

Plasmid

#42940

Depositor

Eline Luning Prak

Article

Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

Insert

Human L1 element (L1RE1 Human)

Use

Tags

EGFP (opposite strand)

Expression

Mammalian

Mutation

Promoter

CMV MIE, L1 5'UTR

Available since

April 25, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-GR11

Plasmid

#89105

Purpose

mammalian expression of full-length human glucocorticoid receptor in pCMV5

Depositor

Elizabeth Wilson

Article

Kupfer et al J Biol Chem. 1993 Aug 15;268(23):17519-2

Insert

human glucocorticoid receptor (NR3C1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Broccoli-aptNFkB#5

Plasmid

#124362

Purpose

Overexpression of bifunctional circular RNA (Broccoli fluorescence and NFkB binding)

Depositor

Samie Jaffrey

Article

Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

Insert

Tornado-F30-Broccoli-aptNFkB#5

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6+27

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pRNA2-(A)128

Plasmid

#174006

Purpose

Used to generate stable mRNA for heterologous expression experiments (includes polyA tail)

Depositor

Stephen Ikeda

Article

Williams et al Front Neurosci. 2010 Nov 4;4:181. doi: 1

Insert

eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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miniCMV-(mNeonGreen)4-tDeg

Plasmid

#129402

Purpose

(mNeonGreen)4-tDeg fluorogenic protein

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

(mNeonGreen)-tDeg

Use

Tags

Expression

Mammalian

Mutation

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pKJ0377

Plasmid

#228712

Purpose

Mammalian expression of human codon-optimized enhanced Bxb1 (epBxb1 mutant T166R)

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Science 0, eadr6006

Insert

Bxb1_T166R

Use

Tags

Expression

Mammalian

Mutation

T166R

Promoter

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-mutTornado-split-nLuc

Plasmid

#212707

Purpose

Tornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

mutTornado-CVB3-split-nLuc

Use

Tags

Expression

Mutation

Deleted the partial 3'ribozyme sequence in T…

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ0463

Plasmid

#228715

Purpose

Bacterial expression of epT7 RNAP; for protein production and purification

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Science 0, eadr6006

Insert

Sumo_epT7

Use

Tags

Expression

Bacterial

Mutation

T3M/G47A/E643G

Promoter

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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VN-gamma Synuclein

Plasmid

#89733

Purpose

expresses human gamma Synuclein fused to the N-terminal part of Venus

Depositor

Tiago Outeiro

Article

Eckermann et al Biochim Biophys Acta. 2015 Aug;1852(8):1

Insert

SNCG (SNCG Human)

Use

Tags

N-terminal part of Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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UbC-(mNeonGreen)4-tDeg

Plasmid

#129404

Purpose

(mNeonGreen)4-tDeg fluorogenic protein

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

(mNeonGreen)4-tDeg

Use

Tags

Expression

Mammalian

Mutation

Promoter

UbC

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pMUH_unc84_2XGFP

Plasmid

#46023

Depositor

Sean Eddy

Article

Henry et al Nucleic Acids Res. 2012 Oct;40(19):9691-

Insert

UNC-84 (unc-84 )

Use

10xuas-phi31-expression vector

Tags

2XGFP

Expression

Mutation

Two amino acid mutations (F279L and L500P) compar…

Promoter

10XUAS

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pKJ0376

Plasmid

#228711

Purpose

Mammalian expression of human codon-optimized Bxb1 mutant N194G

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Science 0, eadr6006

Insert

Bxb1_N194G

Use

Tags

Expression

Mammalian

Mutation

N194G

Promoter

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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ZipGFP11_TEV

Plasmid

#81243

Purpose

Zipped GFP11 with TEV cleavage sequence

Depositor

Xiaokun Shu

Article

To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

Insert

zipped GFP11 with TEV cleavage sequence

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCEP4 Smad4

Plasmid

#16483

Depositor

Bert Vogelstein

Article

Riggins et al Nat Genet. 1996 Jul . 13(3):347-9.

Insert

Smad4 (SMAD4 Human)

Use

Tags

Double HA

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pWL89A mPing

Plasmid

#145787

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock, Susan Wessler

Article

Hancock et al Mob DNA. 2010 Feb 1;1(1):5. doi: 10.1186

Insert

mPing

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

Jan. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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RP4-7

Plasmid

#79811

Purpose

Modified broad host range plasmid RP4. Chloramphenicol resistant. Helper plasmid

Depositor

Michael Hynes

Article

Quandt et al Plasmid. 2004 Jul;52(1):1-12.

Insert

Chloramphenicol acetyltransferase

Use

Helper plasmid for mobilization

Tags

Expression

Mutation

Promoter

Own promoter

Available since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only
pLV_Stargazin-mTurquoise2-iLID

Plasmid

#161001

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to Stargazin for slow diffusion

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

Stargazin-mTurquoise2-iLID

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-5S-Squash

Plasmid

#177914

Purpose

Expresses 5S rRNA fused to Squash aptamer in mammalian cells

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Squash aptamer

Use

Tags

Expression

Mammalian

Mutation

Promoter

5S

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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