We narrowed to 27,847 results for: Unk

1. pDARMO_CMVT_FLAG-HUWE1

   Plasmid

   #187121

   Purpose

   mammalian cell expression of FLAG-HUWE1

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Tags

   1xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDEST_FLAG-HUWE1_C4341S

   Plasmid

   #187156

   Purpose

   mammalian cell expression FLAG-HUWE C4341S

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Tags

   1xFLAG

   Expression

   Mammalian

   Mutation

   C4341S

   Promoter

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. All_in_one_CRISPR/Cas9_LacZ

   Plasmid

   #74293

   Purpose

   "All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screening

   Depositor

   Lynne Postovit

   Article

   Findlay et al PLoS One. 2016 Apr 18;11(4):e0153901. do

   Inserts

   LacZ-alpha
   Cas9
   mCherry
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Lac promoter, SV40, and T7 promoter

   Available since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-FLEX-SaCas9-U6-sgSlc17a7

   Plasmid

   #124846

   Purpose

   Mutagenesis of Slc17a7

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a7 (Slc17a7 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTET2-RlucCP

   Plasmid

   #198352

   Purpose

   Part of Firefly reporter system, encodes Renilla luciferase

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   Renilla Luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTET2

   Available since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTET2-lucCP

   Plasmid

   #198353

   Purpose

   Part of Renilla reporter system, encodes Firefly luciferase

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   Firefly Luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTET2

   Available since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDARMO_CMVT_3xFLAG-HUWE1_R4187C

   Plasmid

   #187142

   Purpose

   mammalian cell expression of 3xFLAG-HUWE1 R4187C

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   R4187C

   Promoter

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDEST_FLAG-HUWE1_YH/GG

   Plasmid

   #187123

   Purpose

   mammalian cell expression of FLAG-HUWE1 Y355G/H356G

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Tags

   1xFLAG

   Expression

   Mammalian

   Mutation

   Y355G/H356G

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDARMO_CMVT_3xFLAG-HUWE1_H3962D

   Plasmid

   #187131

   Purpose

   mammalian cell expression of 3xFLAG-HUWE1 H3962D

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   H3962D

   Promoter

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pY71-PT7-RiboJ-sfGFP-MGapt

    Plasmid

    #129119

    Purpose

    Expresses a fluorescent reporter for the quantification of cell-free protein expression.

    Depositor

    Matthew Lux

    Article

    Cole et al ACS Synth Biol. 2019 Sep 20;8(9):2080-20

    Insert

    Superfolder GFP with Malachite Green aptamer
    

    Use

    Synthetic Biology

    Tags

    His6 and RiboJ Insulator

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a7

    Plasmid

    #124859

    Purpose

    Mutagenesis of Slc17a7

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a7 (Slc17a7 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-20b_HtrA2(delta(133))_S306A_6His

    Plasmid

    #197975

    Purpose

    E. coli expression of HtrA2(delta133)-6His

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    HtrA2 (HTRA2 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    delta(1-133), S306A

    Promoter

    Available since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDARMO_CMVT_3xFLAG_BIRC6_delta(Helix)

    Plasmid

    #197969

    Purpose

    Mammalian cell expression of 3xFLAG-BIRC6_delta(Helix)

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    BIRC6 (BIRC6 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    L1332V, delta(1616-1666)

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDARMO_CMVT_3xFLAG_BIRC6_delta(Helix)_BIRmut

    Plasmid

    #197970

    Purpose

    Mammalian cell expression of 3xFLAG-BIRC6_delta(Helix)/BIRmut

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    BIRC6 (BIRC6 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    C328S, C331S, L1332V, delta(1616-1666)

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNIC-Bio2_MVPI_SMAC(delta(55))_9His

    Plasmid

    #197978

    Purpose

    E. coli expression of MVPI-SMAC(delta(55))_9His

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    Smac (DIABLO Human)
    

    Use

    Tags

    9His

    Expression

    Bacterial

    Mutation

    delta (1-55), A56M

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNIC-Bio2_SMAC(delta(55))_Cys_9His

    Plasmid

    #197977

    Purpose

    E. coli expression of SMAC(delta(55))_Cys-9His

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    Smac (DIABLO Human)
    

    Use

    Tags

    9His

    Expression

    Bacterial

    Mutation

    delta (1-55), ins240C

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR221-HUWE1_UIM-mut

    Plasmid

    #187147

    Purpose

    Entry clone

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Entry clone

    Tags

    Expression

    Mutation

    A1376G/S1382A

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEST_FLAG-HUWE1_F3194S

    Plasmid

    #187140

    Purpose

    mammalian cell expression of FLAG-HUWE1 F3194S

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    F3194S

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDARMO_CMVT_3xFLAG-HUWE1_H669Q

    Plasmid

    #187138

    Purpose

    mammalian cell expression of 3xFLAG-HUWE1 H669Q

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    H669Q

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAC8-HUWE1-HECTonly

    Plasmid

    #187119

    Purpose

    insect cell expression of HUWE1 HECT domain

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    Strep

    Expression

    Insect

    Mutation

    delta1-3992

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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