We narrowed to 270 results for: cdc42

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H2A-mTurquoise-RhoJQ79L-deltaCaax

Plasmid

#176110

Purpose

Constitutively active, nucelar localized RhoJ

Depositor

Dorus Gadella

Publication

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

RhoJ (RHOJ Human)

Expression

Mammalian

Mutation

Q79L

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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TOMM20-mTurquoise-RhoG-QL-deltaCaaX

Plasmid

#176122

Purpose

Constitutively active, mitochondiral localized RhoG

Depositor

Dorus Gadella

Publication

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

RHOG (RHOG Human)

Expression

Mammalian

Mutation

QL

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Ypet-PBD

Plasmid

#105290

Purpose

To measure Rac or Cdc42 activity by binding and FRET analysis

Depositor

Kenneth Yamada

Publication

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

Pak1 (PAK1 Human)

Tags

Ypet

Expression

Mammalian

Mutation

aa encoding 65-150

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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2xsspB-mSc-sspB-ITSN1

Plasmid

#114425

Purpose

Translocating ITSN1 to iLID and activate Cdc42

Depositor

Dorus Gadella

Publication

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

ITSN1

Tags

mScarlet

Expression

Mammalian

Mutation

truncated ITSN1 (1159-1509)

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

Plasmid

#173872

Purpose

Mammalian expression of mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)

Depositor

Takao Nakata

Publication

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAV0757

Plasmid

#133509

Purpose

Expression of 3GFP tagged CRIB domain to monitor active Cdc42

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pHis5(StuI)-p(pak1)-CRIB(gic2aa2-181)-3GFP-terminator(ScADH1)-kanMX

Expression

Yeast

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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mMaroon-PAK-mMaroon

Plasmid

#84361

Purpose

FRET acceptor for Cdc42 donor, flanked with the far-red FP mMaroon

Depositor

Ryohei Yasuda

Publication

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mMaroon-PAK-mMaroon

Tags

mMaroon flanking PAK domain on N and C terminus

Expression

Mammalian

Promoter

CMV

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-mPar6a WT

Plasmid

#86148

Purpose

FLAG tagged mouse Par6 wild type - Binds to aPKC, Par3, Lgl, Cdc42

Depositor

Senthil Muthuswamy

Publication

Nolan et al Cancer Res. 2008 Oct 15;68(20):8201-9.

Insert

Par6 (Pard6a Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

LTR

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only
140_pSKB3-GBD-LUMP

Plasmid

#65972

Purpose

Bacterial expression of GBD-LUMP protein, a sensor for GTP-bound Cdc42

Depositor

Gerard Marriott

Publication

Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

Insert

LUMP

Tags

GBD (GTPase binding domain from ACK1; residues 44…

Expression

Bacterial

Promoter

T7

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pAV0756

Plasmid

#133508

Purpose

Expression of 3mCherry tagged CRIB domain to monitor active Cdc42

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pHis5(StuI)-p(act1)-CRIB(gic2aa2-181)-3mCherry-bsdMX

Expression

Yeast

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-ShadowY-Rhotekin(8-89)-P2A-Clover(T153M/F223R)-RhoA

Plasmid

#165435

Purpose

Expresses ShadowY-Rhotekin(8-89) and Clover(T153M/F223R)-RhoA in mammalian cells to monitor Cdc42 activity with FLIM imaging.

Depositor

Hideji Murakoshi

Publication

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

ShadowY-RBD-P2A-Clover(T153M/F223R)-RhoA

Tags

ShadowY, Clover(T153M/F223R)

Expression

Mammalian

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAV0816

Plasmid

#133510

Purpose

Expression of mTagBFP2 tagged CRIB domain to monitor active Cdc42

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(act1)-CRIB(gic2aa2-181)-mTagBFP2-terminator(ScADH1)-bsdMX

Expression

Yeast

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

Plasmid

#173866

Purpose

Mammalian expression of mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)

Depositor

Takao Nakata

Publication

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mVenus

Expression

Mammalian

Promoter

CMV

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-SspB-HaloTag-ITSN1-DHPH

Plasmid

#176128

Purpose

Heterodimerization with iLID, Cdc42 activation

Depositor

Dorus Gadella

Publication

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

ITSN1 (ITSN1 Human)

Use

Lentiviral

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-RhoA

Plasmid

#12202

Depositor

Jonathan Chernoff

Publication

Bagrodia et al J Biol Chem. 1995 Sep 29. 270(39):22731-

Insert

RhoA (RHOA Human)

Tags

GST

Expression

Bacterial

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pGEX-Rac1

Plasmid

#12200

Depositor

Jonathan Chernoff

Publication

Bagrodia et al J Biol Chem. 1995 Sep 29. 270(39):22731-

Insert

Rac1 (RAC1 Human)

Tags

GST

Expression

Bacterial

Mutation

Starting methionine (M1) removed; F78S compared t…

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pWZL Neo Myr Flag TNK2

Plasmid

#20654

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

TNK2 (TNK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCAG-mCherry-FKBP-GEF(ITSN1)

Plasmid

#222631

Purpose

Cdc42 activation using rapamycin

Depositor

Arnold Hayer

Publication

Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

Insert

mCherry-FKBP-GEF(ITSN1) (ITSN1 Human)

Tags

mCherry-FKBP

Expression

Mammalian

Promoter

CAG

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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SspB-HaloTag-DHPH-ITSN1

Plasmid

#176113

Purpose

Heterodimerization with iLID, Cdc42 activation

Depositor

Dorus Gadella

Publication

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

ITSN1 (ITSN1 Human)

Expression

Mammalian

Available since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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SspB-iRFP-DHPH-ITSN1

Plasmid

#176117

Purpose

Heterodimerization with iLID, Cdc42 activation

Depositor

Dorus Gadella

Publication

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

ITSN1 (ITSN1 Human)

Expression

Mammalian

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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