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pVenus-CyclinB1(R42A) (1317)

Plasmid

#39873

Depositor

Jonathon Pines

Article

Hagting et al J Cell Biol. 2002 Jun 24;157(7):1125-37.

Insert

CyclinB1(R42A) (CCNB1 Human)

Tags

Venus

Expression

Mammalian

Mutation

R42A = destruction box mutations = non-destructib…

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
PSUC2-GFPantisense-CYC1t-pRS413

Plasmid

#64404

Purpose

SUC2 regulated expression of an antisense RNAi construct for GFP

Depositor

Claudia Vickers

Article

Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

Insert

GFPantisense

Expression

Yeast

Promoter

SUC2

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pIS1 Cyclin D2 short UTR

Plasmid

#21644

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Cyclin D2 short UTR (CCND2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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POT5-CYC1p-ycrtYB-ADH1t

Plasmid

#65360

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

CYC1p-ycrtYB-ADH1t

Use

Unspecified

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV Cyclin D2-long-mut

Plasmid

#21657

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Cyclin D2 (CCND2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

mutated miR-15/16 and let-7 binding sites

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pVenus-N1 cyclin B1 L45A (1324)

Plasmid

#61851

Purpose

mammalian expression of cyclin B1 L45 A mutant fused to Venus

Depositor

Jonathon Pines

Article

Matsusaka et al Biol Open. 2014 Sep 12;3(10):904-12. doi

Insert

cyclin B1 (CCNB1 Human)

Tags

Venus

Expression

Mammalian

Mutation

L45A

Promoter

CMV

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only
POT2-CYC1p-ycrtE-ADH1t

Plasmid

#65354

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

CYC1p-ycrtE-ADH1t

Use

Unspecified

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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POT4-CYC1p-ycrtI-ADH1t

Plasmid

#65357

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

CYC1p-ycrtI-ADH1t

Use

Unspecified

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2 SV40 Cyclone-RLuc

Plasmid

#247494

Purpose

Acyclovir-regulated Renilla luciferase

Depositor

Samie Jaffrey

Article

Hou et al Nat Methods. 2025 Nov 3. doi: 10.1038/s4

Insert

Renilla luciferase gene with Cyclone insertion

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Mutation

Cyclone was inserted after Q288 of Renilla lucife…

Promoter

SV40

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 CMV Cyclone-EGFP

Plasmid

#247495

Purpose

Acyclovir-regulated EGFP

Depositor

Samie Jaffrey

Article

Hou et al Nat Methods. 2025 Nov 3. doi: 10.1038/s4

Insert

EGFP gene with Cyclone insertion

Use

Synthetic Biology

Expression

Mammalian

Mutation

Cyclone was inserted after Glutamine94 of EGFP to…

Promoter

CMV

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Cyclone-Cre C-DYK

Plasmid

#247496

Purpose

Acyclovir-regulated Cre

Depositor

Samie Jaffrey

Article

Hou et al Nat Methods. 2025 Nov 3. doi: 10.1038/s4

Insert

Cre with Cyclone insertion

Use

Cre/Lox and Synthetic Biology

Tags

FLAG

Expression

Mammalian

Mutation

Cyclone was inserted after Methionine300 of Cre t…

Promoter

CMV

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pBIG1e-CDK12-KiD/Cyclin K

Plasmid

#231743

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Inserts

CDK12 (CDK12 Human)
CCNK (CCNK Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(700-1082)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP267: pHelper(CyCAST)_PSP1_ΔTniQ

Plasmid

#200842

Purpose

Inducible expression of CyCAST proteins (except TniQ) with crRNA targeting CyPSP1.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP269: pHelper(CyCAST)_PSP1_ΔTnsD

Plasmid

#200844

Purpose

Inducible expression of CyCAST proteins (except TnsD) with crRNA targeting CyPSP1.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP116: pHelper(CyCAST)_PSP1

Plasmid

#200840

Purpose

Inducible expression of CyCAST proteins with crRNA targeting CyPSP1.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-1:ChrA-C63S-C66S

Plasmid

#225082

Purpose

Expresses precursor peptide ChrA-C63S/C66S in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrA-C63S/C66S

Tags

His6

Expression

Bacterial

Mutation

C63S/C66S

Promoter

T7

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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YIplac128-TEF2p-LDH-CYC1t

Plasmid

#216120

Purpose

Yeast integrative vector for constitutive expression of LDH at the LEU2 locus

Depositor

Yvonne Nygård

Article

Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

Insert

Lactate dehydrogenase (LDH Bovine)

Expression

Yeast

Promoter

TEF2 promoter

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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YIplac204-TDH3p-LDH-CYC1t

Plasmid

#216121

Purpose

Yeast integrative vector for constitutive expression of LDH at the TRP1 locus

Depositor

Yvonne Nygård

Article

Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

Insert

Lactate dehydrogenase (LDH Bovine, Bos taurus)

Expression

Yeast

Promoter

TDH3 promoter

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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YIplac204-TDH3p-CDC19-CYC1t

Plasmid

#216122

Purpose

Yeast integrative vector for constitutive expression of CDC19 at the TRP1 locus

Depositor

Yvonne Nygård

Article

Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

Insert

Pyruvate kinase 1

Expression

Yeast

Promoter

TDH3 promoter

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-AtTIR1-Myc-MBP-HA

Plasmid

#222709

Purpose

Expression AtTIR1-Myc and MBP-HA in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Insert

AtTIR1 (TIR1 Mustard Weed)

Tags

myc

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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