We narrowed to 4,074 results for: gast

1. pX330.sgNf1.4

   Plasmid

   #92028

   Purpose

   pX330 sgNf1.4 for Nf1 deletion

   Depositor

   Wen Xue

   Article

   Song et al Gastroenterology. 2017 Apr;152(5):1161-1

   Insert

   sgNf1.4
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Available Since

   Nov. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

2. 6xHis(TEV)-dDrp1-StrepII

   Plasmid

   #174421

   Purpose

   Expresses D. melanogaster Drp1 in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1 (Drp1 Fly)
   

   Tags

   6xHis(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. dmBACCS2VL-IRES-dOrai

   Plasmid

   #67629

   Purpose

   Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOrai

   Depositor

   Takao Nakata

   Article

   Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

   Insert

   dmBACCS2VL-IRES-dOrai
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Hy_pMT Flag-Hel25E WT

   Plasmid

   #110123

   Purpose

   Expresses Flag-tagged D. melanogaster Hel25E

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Hel25E (Hel25E Fly)
   

   Tags

   Flag

   Expression

   Insect

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Oct. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. dmBACCS2NS-IRES-dOrai

   Plasmid

   #67628

   Purpose

   Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOrai

   Depositor

   Takao Nakata

   Article

   Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

   Insert

   dmBACCS2NS-IRES-dOrai
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSTAP-seq_fly-zfh1_spikeIn-1

   Plasmid

   #86387

   Purpose

   Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster zfh1 enhancer & D. pseudoobscura eve core promoter
   

   Use

   Stap-seq spike in vector

   Available Since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSTAP-seq_fly-zfh1_spikeIn-3

   Plasmid

   #86389

   Purpose

   Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#1
   

   Use

   Stap-seq spike in vector

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSTAP-seq_fly-zfh1_spikeIn-4

   Plasmid

   #86390

   Purpose

   Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#2
   

   Use

   Stap-seq spike in vector

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSTAP-seq_fly-zfh1_spikeIn-2

   Plasmid

   #86388

   Purpose

   Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster zfh1 enhancer & D. pseudoobscura CG32369 core promoter
   

   Use

   Stap-seq spike in vector

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWPI-N1ICD

    Plasmid

    #185525

    Purpose

    Constitutive overexpression of human NOTCH1 intracellular domain

    Depositor

    Sean Palecek, Eric Shusta

    Article

    Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

    Insert

    NOTCH1 intracellular domain (NOTCH1 Human)
    

    Use

    Lentiviral

    Promoter

    EF-1a

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUAST attB Orco (Y478A)

    Plasmid

    #73928

    Purpose

    UAS induced expression of Drosophila melanogaster Orco cDNA with 478 Tyrosine to Alanine amino acid change

    Depositor

    Leslie Vosshall

    Article

    Nakagawa et al PLoS One. 2012;7(3):e32372. doi: 10.1371

    Insert

    Orco (Y478A) (Orco Fly)
    

    Use

    Unspecified

    Mutation

    changed 478 Tyrosine for Alanine

    Available Since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDmAct5C::Cas9-2A-Neo

    Plasmid

    #176679

    Purpose

    Expression of Drosophila codon optimized spCas9 under Drosophila Act5C promoter

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Insert

    spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
    

    Use

    Crispr

    Expression

    Insect

    Mutation

    Drosophila codon optimized

    Promoter

    D. melanogaster Act5C

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWPI-N3ICD

    Plasmid

    #185524

    Purpose

    Constitutive overexpression of human NOTCH3 intracellular domain

    Depositor

    Sean Palecek, Eric Shusta

    Article

    Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

    Insert

    NOTCH3 intracellular domain (NOTCH3 Human)
    

    Use

    Lentiviral

    Promoter

    EF-1a

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. dmBACCS2-IRES-dOrai-IRES-mCherry

    Plasmid

    #72894

    Purpose

    Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2-IRES-dOrai-IRES-mCherry
    

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCRISPRv2.sgNf1.1

    Plasmid

    #91895

    Purpose

    sgNf1.1 for Nf1 deletion

    Depositor

    Wen Xue

    Article

    Song et al Gastroenterology. 2017 Apr;152(5):1161-1

    Insert

    sgNf1.1
    

    Use

    Lentiviral and Mouse Targeting

    Expression

    Mammalian

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiCRISPRv2.sgNf1.4

    Plasmid

    #92029

    Purpose

    sgNf1.4 for Nf1 deletion

    Depositor

    Wen Xue

    Article

    Song et al Gastroenterology. 2017 Apr;152(5):1161-1

    Insert

    sgNf1.4 for Nf1 deletion
    

    Use

    Lentiviral and Mouse Targeting

    Expression

    Mammalian

    Available Since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC57-white[coffee]

    Plasmid

    #84006

    Purpose

    Donor HR template carrying a 2,080 bp fragment of the Drosophila melanogaster white[coffee] allele and silent mutations conferring resistance to white sgRNAs-1, -2, -3, and -4

    Depositor

    Phillip Zamore

    Article

    Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.

    Insert

    A 2,080 bp fragment of the white[coffee] allele (w Fly)
    

    Use

    Unspecified

    Mutation

    A GC-to-AA mutation that creates a G589E missense…

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. dmBACCS2-IRES-dOrai-IRES-GFP

    Plasmid

    #72893

    Purpose

    Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2-IRES-dOrai-IRES-GFP
    

    Tags

    IRES-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. dmBACCS2NS-IRES-dOrai-IRES-GFP

    Plasmid

    #72895

    Purpose

    Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2NS-IRES-dOrai-IRES-GFP
    

    Tags

    IRES-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. Hy_pMT Flag-Hel25E K91N

    Plasmid

    #110121

    Purpose

    Expresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activity

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Hel25E (Hel25E Fly)
    

    Tags

    Flag

    Expression

    Insect

    Mutation

    K91N mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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