-
Plasmid#208024PurposeControl biosensor for extracellular L-lactateDepositorInsertdeLACCO1
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-UBE2QL1-Strep/HA
Plasmid#124666PurposeExpresses UBE2QL1 with C-terminal Strep-HA tag in mammalian cellsDepositorInsertUBE2QL1 (UBE2QL1 Human)
UseTags2xStrep/HAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRvCAHS3-CAHS3-mEGFP
Plasmid#205006PurposeThe vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the RvCAHS3-mEGFP gene positioned in between.DepositorInsertRvCAHS3-mEGFP with 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TPI-PTC160
Plasmid#130698PurposeExpresses TPI-PTC160 NMD reporter (It has a Premature termination codon (PTC) at 160th Amino acid)DepositorInsertTPI-PTC160 (TPI1 Human)
UseTagsExpressionMammalianMutationPTC160PromoterPcmvAvailable sinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-mScarlet-I-P2A-iCre (JDW 917)
Plasmid#224516PurposeA Gateway-compatible middle entry clone containing mScarlet-I P2A-iCre (codon optimized)DepositorInsertmScarlet-I-P2A-iCre
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-UBE2QL1-CS-Strep/HA
Plasmid#129513PurposeExpresses UBE2QL1 C88S (catalytically inactive mutant) with C-terminal Strep-HA tag in mammalian cellsDepositorInsertUBE2QL1 C88S (UBE2QL1 Human)
UseTags2xStrep/HAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-SomArchon
Plasmid#126943PurposeCre-dependent expression of SomArchon under CAG promoterDepositorInsertSomArchon
UseAAVTagsGFPExpressionMammalianMutationPromoterCAGAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-lox-3xSTOP-lox (JDW 869)
Plasmid#224521PurposeA Gateway compatible middle entry clone containing a loxP flanked SV40 polyA, bGH polyA, and SV40 pA stop cassetteDepositorInsertLoxP-3xStop-LoxP
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
tetO-TCF21
Plasmid#170706Purposedoxycycline-inducible overexpression of TCF21DepositorInsertTCF21 (TCF21 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET_SZ1-RGG-GFP-RGG
Plasmid#124937PurposeExpression of SZ1-RGG-GFP-RGGDepositorInsertSYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.4
Plasmid#111057PurposeExpresses dLight1.4 in mammalian cellsDepositorInsertdLight1.4
UseTagsFlag tagExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.GCaMP6m.WPRE.SV40
Plasmid#100841PurposeAAV expression of ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6m
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P M378G K379S D380Y T381R S383T …PromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-MAP1LC3B
Plasmid#175753PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-MAP1LC3BDepositorInsertMAP1LC3B (MAP1LC3B Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRvSAHS1-SAHS1-mEGFP
Plasmid#205007PurposeThe vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the RvSAHS1-mEGFP gene positioned in between.DepositorInsertRvSAHS1-mEGFP with 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.Twitch2B.WPRE.SV40
Plasmid#100039PurposeAAV expression of FLEXed Twitch2B driven by CAG promoter. Twitch 2B is a fluorescent reporter for calcium signalingDepositorInsertTwitch2B
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-GEX-GECO1
Plasmid#32443PurposeMammalian expression of green excitation ratiometric genetically encoded Ca2+ indicator for optical imagingDepositorInsertGEX-GECO1.0
UseTagsExpressionMammalianMutationGCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-CALCOCO2
Plasmid#175759PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-CALCOCO2/NDP52DepositorInsertNDP52/CALCOCO2 (CALCOCO2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME-Lifeact-EGFP-3xHA (JDW 1322)
Plasmid#224494PurposeGateway compatible middle entry clone containing Lifeact-EGFP (EGFP F-actin reporter)DepositorInsertLiefact-EGFP-3xHA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobody
Plasmid#138128PurposeExpresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells.DepositorInsertmClover3-anti-mouse-IgG(kappa)-nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-FLIM-AKART391A
Plasmid#60446PurposeExpresses the non-phosphorylatable point mutant (T391A) of FLIM-AKAR in a Cre dependent manner in an AAV backboneDepositorInsertFLIM-AKART391A
UseAAV and Cre/LoxTagsExpressionMammalianMutationchanged Threonine 391 to AnaninePromoterCAGAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHeEf1a-mEGFP-NLS
Plasmid#205012PurposeThe vector contains 1kbp of the upstream and downstream regions of the ef1a gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the ef1a gene from Hypsibius exemplaris
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvACT-mEGFP-NLS
Plasmid#205001PurposeThe vector contains 1kbp of the upstream and downstream regions of the actin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
pAAV.Syn.Flex.GCaMP6m.WPRE.SV40
Plasmid#100838PurposeAAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas ServiceAAV1 and AAV9InsertGCaMP6m
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P M378G K379S D380Y T381R S383T …PromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-GRAB_eCB2.0
Plasmid#164608PurposeExpress the endocannabinoid sensor GRAB_eCB2.0 in CaMKII positive neuronsDepositorInsertEndocannabinoid sensor GRAB_eCB2.0
UseAAVTagsExpressionMutationS383TPromoterCaMKIIAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-Neuromodulin-IRES-Puromycin
Plasmid#134666PurposeExpresses EGFP-tagged plasma membrane markerDepositorInsertNeuromodulin (GAP43 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP1-N1-SialT
Plasmid#87324PurposeTo express SialT-AcGFP1 in mammalian cells, SialT encodes the full length coding sequence of β-galactosamide α-2,6-sialyltransferaseDepositorInsertST 6 beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1 Human)
UseTagsAcGFP1ExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-AlstR-IRES2-EGFP
Plasmid#14895DepositorInsertAlstR (AstA-R1 Fly)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2007AvailabilityAcademic Institutions and Nonprofits only -
pyc111f
Plasmid#158780Purposeribosome-tagged, Cre-dependent GCaMP for soma localizationDepositorInsertGCaMP6f-RPL10a
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-SomArchon
Plasmid#126942PurposeExpression of SomArchon under CaMKII promoterDepositorInsertSomArchon
UseAAVTagsGFPExpressionMammalianMutationPromoterCaMKIIAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only