Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 12,598 results for: 112

Showing: 6451 - 6500 of 12598 results

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ClhN-p1k-Cox4-V5-APEX2-Ura

Plasmid

#207061

Purpose

Expressing Cox4-V5-APEX2. Marker for mitochondrial inner membrane. Tends to generate facture in thin resin sections.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Cox4

Tags

V5-APEX2

Expression

Yeast

Promoter

pCOX4

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ClhN-p1k-Cox4-GFP-APEX2-Ura

Plasmid

#207062

Purpose

Expressing Cox4-GFP-APEX2. GFP interferes with APEX staining.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Cox4

Tags

GFP-APEX2

Expression

Yeast

Promoter

pCOX4

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ClhN-pERG6-Pex3-4V5-APEX2-URA

Plasmid

#207068

Purpose

Moderate overexpression of Pex3-4V5-APEX2 under ERG6 promoter. Marker for peroxisomes.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Pex3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pERG6

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ClhN-pLSP1-Pex3-4V5-APEX2-URA

Plasmid

#207069

Purpose

Moderate overexpression of Pex3-4V5-APEX2 under LSP1 promoter. Marker for peroxisomes.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Pex3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pLSP1

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ClhN-p1k-Tgl3-4V5-APEX2-URA

Plasmid

#207059

Purpose

Expressing Tgl3-4V5-APEX2. Insufficient signal for EM.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Tgl3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pTGL3

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ClhN-p1k-Pex3-4V5-APEX2-URA

Plasmid

#207057

Purpose

Expressing Pex3-4V5-APEX2. Insufficient signal for EM.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

PEX3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pPEX3

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKJ238

Plasmid

#212321

Purpose

Express KnFnuc guide with pureexpress

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

KnFunc guide

Expression

Bacterial

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mpeg:Rab5c-stop-polyA; cmlc2:GFP

Plasmid

#213737

Purpose

Expresses mCherry-Rab5c in the macrophage lineage of zebrafish with a green heart transgenesis marker

Depositor

William Talbot

Article

Iyer et al Sci Adv. 2022 Sep 2;8(35):eabp8321. doi:

Insert

mCherry-Rab5c

Use

Zebrafish

Tags

mCherry

Promoter

mpeg1.1

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHB1

Plasmid

#204668

Purpose

pUC19 containing the LL-FRT-erm-FRT-spacer sequence which serves as template for BarSeq deletion constructs

Depositor

Mark Mandel

Article

Burgos et al mSystems. 2020 Dec 15;5(6):e00846-20. do

Insert

FRT-erm-FRT

Use

Synthetic Biology

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-6xHis-GFP(+6)-L1

Plasmid

#205039

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(+6)-L1

Tags

GRRGKKSRK and MGHHHHHGGA

Expression

Bacterial

Mutation

E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-6xHis-GFP(+6)-L2

Plasmid

#205040

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(+6)-L2

Tags

GRRGKKSRKGRRGKKSRK and MGHHHHHGGA

Expression

Bacterial

Mutation

E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-6xHis-GFP(+6)-L3

Plasmid

#205041

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(+6)-L3

Tags

GRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGA

Expression

Bacterial

Mutation

E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-6xHis-GFP(+6)-H1

Plasmid

#205042

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(+6)-H1

Tags

KRRRKK and MGHHHHHGGA

Expression

Bacterial

Mutation

E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-6xHis-GFP(+6)-H2

Plasmid

#205043

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(+6)-H2

Tags

KRRRKKKRRRKK and MGHHHHHGGA

Expression

Bacterial

Mutation

E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-6xHis-GFP(+6)-H3

Plasmid

#205044

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(+6)-H3

Tags

KRRRKKKRRRKKKRRRKK and MGHHHHHGGA

Expression

Bacterial

Mutation

E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ClhN-pTPI1-Emc1-GFP-APEX2-Ura

Plasmid

#207074

Purpose

Expressing Emc1-GFP-APEX2. GFP interferes with APEX staining.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Emc1

Tags

GFP-APEX2

Expression

Yeast

Promoter

pTPI1

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ClhN-pTPI1-Cox4-GFP-APEX2-Ura

Plasmid

#207075

Purpose

Expressing Cox4-GFP-APEX2. GFP interferes with APEX staining.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Cox4

Tags

GFP-APEX2

Expression

Yeast

Promoter

pTPI1

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-GFP-2A-Puro-gRAD9A_B

Plasmid

#211612

Purpose

sgRNA-B against RAD9

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA RAD9A

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-GFP-2A-Puro-gRHNO1_B

Plasmid

#211614

Purpose

sgRNA-B against Rhno

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA RHNO1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-GFP-2A-Puro-gRHNO1_A

Plasmid

#211613

Purpose

sgRNA-A against Rhno

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA RHNO1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-GFP-2A-Puro-gETAA1_A

Plasmid

#211539

Purpose

sgRNA-A against ETAA1

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA ETAA1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-GFP-2A-Puro-gETAA1_B

Plasmid

#211540

Purpose

sgRNA-B against ETAA1

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA ETAA1

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-GFP-2A-Puro-gATRIP_B

Plasmid

#211538

Purpose

sgRNA-B against ATRIP

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA ATRIP

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-GFP-2A-Puro-gRAD1_B

Plasmid

#211545

Purpose

sgRNA-B against RAD1

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA RAD1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-GFP-2A-Puro-gHUS1_A

Plasmid

#211541

Purpose

sgRNA-A against HUS1

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA HUS1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-GFP-2A-Puro-gRAD9A_A

Plasmid

#211611

Purpose

sgRNA-A against RAD9

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA RAD9A

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-GFP-2A-Puro-gRAD1_A

Plasmid

#211544

Purpose

sgRNA-A against RAD1

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA RAD1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-GFP-2A-Puro-gHUS1_B

Plasmid

#211542

Purpose

sgRNA-B against HUS1

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA HUS1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-GFP-2A-Puro-gIL25_A

Plasmid

#211543

Purpose

sgRNA-A against IL25

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA IL25

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKJ217

Plasmid

#212318

Purpose

Express MmFnuc with pureexpress

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

Mercenaria

Expression

Bacterial

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIGII-TCR-F

Plasmid

#194392

Purpose

Retroviral expression of candidate orphan TCR

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

TCR-F (Tcra, Tcrb Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIGII-TCR-G

Plasmid

#194393

Purpose

Retroviral expression of candidate orphan TCR

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

TCR-G (Tcra, Tcrb Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIAII-TCR-A

Plasmid

#194394

Purpose

Retroviral expression of candidate orphan TCR

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

TCR-A (Tcra, Tcrb Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIAII-TCR-B

Plasmid

#194395

Purpose

Retroviral expression of candidate orphan TCR

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

TCR-B (Tcra, Tcrb Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIAII-TCR-C

Plasmid

#194396

Purpose

Retroviral expression of candidate orphan TCR

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

TCR-C (Tcra, Tcrb Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIAII-TCR-D

Plasmid

#194397

Purpose

Retroviral expression of candidate orphan TCR

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

TCR-D (Tcra, Tcrb Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIAII-TCR-E

Plasmid

#194398

Purpose

Retroviral expression of candidate orphan TCR

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

TCR-E (Tcra, Tcrb Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIAII-TCR-F

Plasmid

#194399

Purpose

Retroviral expression of candidate orphan TCR

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

TCR-F (Tcra, Tcrb Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIAII-TCR-G

Plasmid

#194400

Purpose

Retroviral expression of candidate orphan TCR

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

TCR-G (Tcra, Tcrb Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIGII-TCR-A

Plasmid

#194387

Purpose

Retroviral expression of candidate orphan TCR

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

TCR-A (Tcra, Tcrb Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIGII-TCR-B

Plasmid

#194388

Purpose

Retroviral expression of candidate orphan TCR

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

TCR-B (Tcra, Tcrb Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIGII-TCR-C

Plasmid

#194389

Purpose

Retroviral expression of candidate orphan TCR

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

TCR-C (Tcra, Tcrb Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIGII-TCR-D

Plasmid

#194390

Purpose

Retroviral expression of candidate orphan TCR

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

TCR-D (Tcra, Tcrb Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pWR95

Plasmid

#203916

Purpose

BLINCAR plasmid for gene deletion, no genomic target

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

modified MCS

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWT35S-TP-UnaG

Plasmid

#203484

Purpose

For particle bombardment. A plastid transit peptide fused UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Plastid transit peptide fused UnaG

Expression

Bacterial and Plant

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXSIP-TMEM141-FLAG

Plasmid

#204702

Purpose

expresses TMEM141-FLAG in mammalian cells

Depositor

William Kaelin

Article

Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

Insert

TMEM141 (TMEM141 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6.3/TO/V5-DEST-mTurquoise2

Plasmid

#191010

Purpose

Lentiviral expression vector for mTurquoise2

Depositor

Johanna Ivaska

Article

Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

Insert

mTurquoise2

Use

Lentiviral

Promoter

CMV

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBetaActin-dCer3-C2-FRB

Plasmid

#191327

Purpose

Expresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-C2-dCer3

Expression

Mammalian

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB2488

Plasmid

#190373

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::IcaR-NLS_19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::IcaR-NLS_19St

Expression

Plant

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB2487

Plasmid

#190372

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::AmtR-NLS_19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::AmtR-NLS_19St

Expression

Plant

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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