We narrowed to 4,521 results for: GCA;

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pU6-SAG1-DHFR

Plasmid

#80322

Purpose

Encodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamine

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

SAG1 sgRNA

Use

CRISPR

Promoter

U6

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pX330 Human 3' HP1a gRNA

Plasmid

#127907

Purpose

WT Cas9 Vector targeting the 3' end of the human HP1a gene

Depositor

Mark Groudine

Article

Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

Insert

gRNA for Human 3' HP1a

Use

CRISPR

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRB-33-MC_hammer

Plasmid

#138990

Purpose

IVT template for the beta subunit of the mouse TCR #33 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRB (Tcrb Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRA-33-MC_hammer

Plasmid

#138989

Purpose

IVT template for the alpha subunit of the mouse TCR #33 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRA (Tcra Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRB-32-riot_punisher

Plasmid

#138988

Purpose

IVT template for the beta subunit of the mouse TCR #32 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRB (Tcrb Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRA-32-riot_punisher

Plasmid

#138987

Purpose

IVT template for the alpha subunit of the mouse TCR #32 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRA (Tcra Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRB-31-murderous_crow

Plasmid

#138986

Purpose

IVT template for the beta subunit of the mouse TCR #31 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRB (Tcrb Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pEasyG2_mic

Plasmid

#184916

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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sh-RNA hAtg13

Plasmid

#31971

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

Atg13 (ATG13 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
RRM1 E1.3 gRNA

Plasmid

#90880

Purpose

3rd generation lentiviral gRNA plasmid targeting human RRM1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

RRM1 (Guide Designation E1.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_PELO

Plasmid

#127124

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA PELO (PELO Human)

Use

CRISPR

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCFD3-ovoD1

Plasmid

#111142

Purpose

Expresses U6:3-sgRNA targeting ovoD1 mutation in Drosophila melanogaster

Depositor

Norbert Perrimon

Article

Ewen-Campen et al G3 (Bethesda). 2018 Jul 31;8(8):2749-275

Insert

pCFD3-ovoD1 (ovo Fly)

Use

CRISPR

Expression

Insect

Promoter

U6:3

Available Since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Dnmt3b-R

Plasmid

#122333

Purpose

Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Dnmt3b

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pX330.sgNf1.4

Plasmid

#92028

Purpose

pX330 sgNf1.4 for Nf1 deletion

Depositor

Wen Xue

Article

Song et al Gastroenterology. 2017 Apr;152(5):1161-1

Insert

sgNf1.4

Use

Mouse Targeting

Expression

Mammalian

Available Since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only
pLentiCRISPRpuro-sgROSA26

Plasmid

#231562

Purpose

pLentiCRISPR expression vector for Cas9 and gRNA targeting human ROSA26 locus (Puromycin selection marker)

Depositor

Rizwan Haq

Article

Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e

Insert

sgROSA26

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF-1a

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgAMPK alpha1 clone1

Plasmid

#162119

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha1 (PRKAA1 Human)

Use

Lentiviral

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Stk11)-PGKpuroBFP-W

Plasmid

#105043

Purpose

Lentiviral gRNA plasmid targeting mouse Stk11 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Stk11 (Stk11 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G3_Dual_sgRNA

Plasmid

#173202

Purpose

Coselection for PE3 or HDR in human cells. Vector for tandem expression of ATP1A1 G3 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G3 sgRNA + user-specified sgRNA

Use

CRISPR; Prime editing

Expression

Mammalian

Promoter

Tandem U6 promoters

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pRDA_936

Plasmid

#216094

Purpose

Cas12a [EnAs] knockout targeting CD46, CD47, CD55, CD81, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD46, CD47, CD55, CD81 guides

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-sh-mSlug-4

Plasmid

#40648

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSlug-4 (Snai2 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only