We narrowed to 8,377 results for: PAN

1. pET28a-Ec.coaE (pESC124)

   Plasmid

   #50390

   Purpose

   Heterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.

   Depositor

   Tadhg Begley, Erick Strauss

   Article

   Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

   Insert

   DPCK
   

   Use

   Tags

   6XHIS tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEVA63_Helper

   Plasmid

   #190432

   Purpose

   M13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ297.)

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Insert

   M13 genes: I-XI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. p177bp-LacOx162-Hyg

   Plasmid

   #210215

   Purpose

   Integrates 162xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Insert

   162xLacO repeats derived from: PMID: 12864855.
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

   Plasmid

   #45565

   Purpose

   Expresses I-Sce I nuclease in mammalian cells and BFP as a tracker

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Inserts

   I-Sce I Nuclease
   BFP
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDD285

   Plasmid

   #66826

   Purpose

   mKate2^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

   Insert

   mKate2-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans codon-optimized mKate2

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pGWB701NL3F10H

   Plasmid

   #141288

   Purpose

   No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, Tunicamycin R for plant)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Type

   Empty backbone

   Use

   Luciferase; Gateway

   Tags

   FLAG (x3), HIS (x10), and NLUC

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pH-PABE-7-esgRNA

   Plasmid

   #115620

   Purpose

   Targeted A to G in rice

   Depositor

   Caixia Gao

   Article

   Li et al Genome Biol. 2018 May 29;19(1):59. doi:

   Insert

   wtTadA-TadA7.10-nCas9-3*NLS
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTSA-13

   Plasmid

   #17327

   Depositor

   Takeshi Sano

   Article

   Sano et al J Biol Chem. 1995 Nov 24. 270(47):28204-

   Insert

   streptavidin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Truncated to include only amino acids 16-133 of m…

   Promoter

   Available since

   Feb. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-pDisplay-Halo

   Plasmid

   #223497

   Purpose

   Expression of HaloTags to outer surface in mammalian cells

   Depositor

   James Frank

   Article

   Tobias et al Chem Sci. 2021 Sep 15;12(40):13506-13512

   Insert

   HaloTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCfB2189

    Plasmid

    #67532

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae X-3 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. YIplac211-ABP1-iGFPx6

    Plasmid

    #177099

    Purpose

    Expresses Abp1-iGFPx6 in yeast cells

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

    Insert

    ABP1 (ABP1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CMV-pDisplay-SNAP

    Plasmid

    #223495

    Purpose

    Expression of SNAP-tags to outer surface in mammalian cells

    Depositor

    James Frank

    Article

    Tobias et al Chem Sci. 2021 Sep 15;12(40):13506-13512

    Insert

    SNAP-tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEC2735 (pSpy0C2)

    Plasmid

    #220279

    Purpose

    integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombination

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMK485 (3mAID-Hygro)

    Plasmid

    #214397

    Purpose

    3mAID for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    3mAID-Hygro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA 3.3 EGFP

    Plasmid

    #115786

    Purpose

    Expresses EGFP in mammalian cells

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCfB2225

    Plasmid

    #67553

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XII-2 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMK448 (BromoTag-BSD)

    Plasmid

    #214378

    Purpose

    BromoTag for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BromoTag-BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-HASAP

    Plasmid

    #138325

    Purpose

    chemigenetic voltage indicator

    Depositor

    Eric Schreiter

    Article

    Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

    Insert

    HASAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC_sgRNA

    Plasmid

    #68710

    Purpose

    Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.

    Depositor

    Roberto Docampo

    Article

    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBT373

    Plasmid

    #125614

    Purpose

    Expresses FERNY-BE4max-NG in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    FERNY-BE4max-NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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