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shPBRM1#32-Tet-pLKO-puro

Plasmid

#107399

Purpose

Lentiviral expression of shRNA targeting PBRM

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

shRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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shPBRM1#131-Tet-pLKO-puro

Plasmid

#107400

Purpose

Lentiviral expression of shRNA targeting PBRM

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

shRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pDS-RED-C1-Beclin1

Plasmid

#24405

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Tags

DsRed

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

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pSLIK-Zeo

Plasmid

#25736

Purpose

Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Zeocin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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retro-gRNA-mRFP1

Plasmid

#112914

Purpose

Retrovirus expressing mRFP1 with BBSI cloning sites for sgRNA

Depositor

Christophe Benoist, Diane Mathis

Article

Magnuson et al Proc Natl Acad Sci U S A. 2018 Nov 6;115

Insert

mRFP1

Use

Retroviral

Promoter

EF1a

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-MEKK1-1-1493

Plasmid

#21632

Depositor

Melanie Cobb

Article

Xu et al Proc Natl Acad Sci U S A. 1995 Jul 18. 9

Insert

mitogen-activated protein kinase kinase kinase 1 (Map3k1 Rat)

Tags

HA

Expression

Mammalian

Available Since

Feb. 8, 2010

Availability

Academic Institutions and Nonprofits only
pGal4x5-E4T-G210

Plasmid

#171077

Purpose

The resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permission

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

Gal4-E4-Gless-cassette

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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ClCry4 Y319D

Plasmid

#160649

Purpose

Expression of protein for various experiments including finding interaction partners

Depositor

Joseph Takahashi

Article

Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

Insert

Clcry4 PHR Y319D

Tags

Hexa his

Expression

Bacterial

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pKnockinDonor-human-p16INK4A-P2A-mCherry

Plasmid

#137026

Purpose

donor vector to knock in P2A-mCherry to the C-terminus of endogenous human p16INK4A

Depositor

Chunliang Li

Article

Zhang et al Proc Natl Acad Sci U S A. 2019 Dec 9. pi

Insert

p16INK4A-knockin-homology arm-P2A-mCherry

Tags

P2A-mCherry

Expression

Bacterial

Promoter

no promoter

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Atg5

Plasmid

#24922

Depositor

Toren Finkel

Article

Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

Insert

Atg5 (ATG5 Human)

Tags

Myc

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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pMKO.1 puro hTERT shRNA

Plasmid

#10688

Depositor

William Hahn

Article

Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

Insert

hTERT shRNA (TERT Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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sgPBRM1-1- LentiCRISPRv2

Plasmid

#107403

Purpose

Lentiviral expression of Cas9 and gRNA targeting PBRM1

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

gRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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sgPBRM1-2- LentiCRISPRv2

Plasmid

#107404

Purpose

Lentiviral expression of Cas9 and gRNA targeting PBRM1

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

gRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBSFI-myr-akt-T308D/S473D

Plasmid

#49192

Purpose

cDNA of Akt1 with myristyolation signal and point mutation from T to D at position 308 and S to D at position 473 in cloning vector

Depositor

Peter Vogt

Article

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Mutation

T308D; S473D

Promoter

T7

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pORTMAGE312B

Plasmid

#128969

Purpose

Broad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid induction

Depositor

George Church

Article

Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

Inserts

PapRecT
mutL E32K

Expression

Bacterial

Mutation

E32K

Promoter

xylS-Pm

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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Orai1-jGCaMP8f

Plasmid

#231630

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2023 Sep 26;12

Insert

Orai1-jGCaMP8f (ORAI1 Synthetic, Human)

Tags

jGCaMP8f

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pLEGFP-S727A-STAT3

Plasmid

#71446

Purpose

Retroviral expression of S727A-STAT3. Please note that this plasmid contains S727A-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Article

Dasgupta et al Proc Natl Acad Sci U S A. 2015 Mar 31;11

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Mutation

S727A

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Barr2 flag

Plasmid

#14685

Depositor

Robert Lefkowitz

Article

Laporte et al Proc Natl Acad Sci U S A. 1999 Mar 30. 9

Insert

beta-arrestin 2 (Arrb2 Rat)

Tags

flag

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
pSP64-hERG1a

Plasmid

#53051

Purpose

Expresses alpha subunit of hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Promoter

SP6

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-GT

Plasmid

#26198

Depositor

Edward Callaway

Article

Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Enhanced green fluorescent protein, TVA (eGFP Mouse, TVA is a mouse codon optimized version of an avian gene.)

Use

AAV and Cre/Lox

Mutation

TVA mammalian codon optimized

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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