We narrowed to 11,726 results for: pcDNA

1. pcDNA3-SypHluorin 1x (S1x)

   Plasmid

   #37003

   Depositor

   Yongling Zhu, Stephen Heinemann

   Article

   Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

   Insert

   SypHluorin (Syp Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   One copy of pHluorin inserted at site 184-185

   Promoter

   CMV

   Available since

   July 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-CTLA4-mgSrtA

   Plasmid

   #125794

   Purpose

   for co-expression of CTLA4 and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of CD80/86 positive cells.

   Depositor

   P. Chen

   Article

   Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

   Insert

   CTLA4-mgSrtA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-ExRai-CKAR(T/A)

   Plasmid

   #118410

   Purpose

   Negative-control mutant for ExRai-CKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-CKAR
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1/Hygro(-)-B2-TS5

   Plasmid

   #104354

   Purpose

   Expresses human beta 2 integrin tension sensor

   Depositor

   Timothy Springer, Pontus Nordenfelt

   Article

   Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

   Insert

   Human integrin b2 with tension sensor (ITGB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-FLAG-GPD1

   Plasmid

   #32487

   Depositor

   Pere Puigserver

   Article

   Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

   Insert

   GPD1 (Gpd1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.2-V5-DEST-hSFRP1-VF

   Plasmid

   #189542

   Purpose

   Protein expression of SFRP1 in mammalian cells.

   Depositor

   Haian Fu

   Insert

   secreted frizzled related protein 1 (SFRP1 Human)
   

   Use

   Tags

   VF

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pF154 pcDNA3.1(+)SOD1G85R

   Plasmid

   #26400

   Depositor

   Elizabeth Fisher

   Article

   Stevens et al PLoS One. 2010 . 5(3):e9541.

   Insert

   SOD1 (SOD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G85R

   Promoter

   Available since

   Nov. 23, 2010

   Availability

   Academic Institutions and Nonprofits only

8. alpha-0 CFP in pcDNA3.1

   Plasmid

   #36396

   Depositor

   Narasimhan Gautam

   Article

   Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

   Insert

   G protein alpha 0 (Gnao1 Rat)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   CFP inserted into the KpnI-SpeI sites engineered …

   Promoter

   cmv

   Available since

   Feb. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pCDNA-3xFLAG-TCAB1

   Plasmid

   #51630

   Purpose

   Mammalian expression of 3xFLAG tagged TCAB1, N-terminal.

   Depositor

   Steven Artandi

   Article

   Venteicher et al Science. 2009 Jan 30;323(5914):644-8. do

   Insert

   TCAB1 (WRAP53 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-mRuby2-MACROD1_d77

    Plasmid

    #172588

    Purpose

    For transient expression of C-terminal mRuby2-tagged MACROD1del1-77

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    MACROD1 (MACROD1 Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-76

    Promoter

    CMV

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO HSPH2

    Plasmid

    #19454

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    heat shock 70kDa protein 4 (HSPA4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutations A381T, T732A, and an insertion of "…

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO V5 DNAJB8

    Plasmid

    #19531

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    DnaJ (Hsp40) homolog, subfamily B, member 8 (DNAJB8 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-FRT-TO-Nsp8

    Plasmid

    #157694

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp8 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pcDNA3.1-MS2-HA-HsSmg7_T

    Plasmid

    #147556

    Purpose

    Mammalian Expression of HsSmg7

    Depositor

    Elisa Izaurralde

    Article

    Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

    Insert

    HsSmg7 (SMG7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    two silent mutation G216A, A1521G and one non sil…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-sACE2v2.4-sfGFP

    Plasmid

    #149666

    Purpose

    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 2.4

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Linker GSGGSGSGG and superfolder GFP

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.2/NS4A-2K-NS4B

    Plasmid

    #120828

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    NS4A-2K-NS4B ZIKV
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pY128 (pcDNA3.1-U6-huMb2Cpf1)

    Plasmid

    #92304

    Purpose

    Expression of humanized Mb2Cpf1 and crRNA

    Depositor

    Feng Zhang

    Insert

    huMb2Cpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3 MDM2 1-400

    Plasmid

    #45754

    Depositor

    Robert Lefkowitz

    Article

    Shenoy et al Science. 2001 Nov 9;294(5545):1307-13. E

    Insert

    MDM2 1-400 (MDM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa 1-400, lacks catalytic RING domain

    Promoter

    CMV

    Available since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1(+)-RABIF-FLAG

    Plasmid

    #135558

    Purpose

    Express human RABIF in mammalian cells, FLAG at N terminal

    Depositor

    Jingshi Shen

    Article

    Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

    Insert

    RABIF (RABIF Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    T7 promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Q769gp120-8his

    Plasmid

    #100929

    Purpose

    Mammalian expression plasmid for gp120 subunit from the Q769.d22 HIV-1 strain

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (Q769.d22) Env
    

    Use

    Tags

    CD5 leader sequence and Purification tag after K5…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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