We narrowed to 13,228 results for: sequence

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pCRISPR2_wt

Plasmid

#149386

Purpose

P. aeruginosa PA14 CRISPR2 locus

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1018-Tier1-PhCMV-p2A

Plasmid

#169491

Purpose

Tier-1 vector encoding a self-cleaving peptides p2a sequence (PhCMV-p2A-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven 2A sequence of porcine teschovirus-1

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMAL-ccDockerin

Plasmid

#72200

Purpose

MBP-ccDockerin expression plasmid

Depositor

Timothy Whitehead

Article

Kowalsky et al Proteins. 2016 Dec;84(12):1914-1928. doi

Insert

Clostridium Cellulolyticum Type I Dockerin

Tags

His-Tag and Maltose Binding Protein

Expression

Bacterial

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCS105

Plasmid

#174830

Purpose

Encodes a unique yeast telomere end at TEL11R by removing the subtelomere

Depositor

Carol Greider

Article

Sholes et al Genome Res. 2022 Apr;32(4):616-628. doi:

Insert

11R Unique

Tags

GAL4 5XUAS

Expression

Bacterial

Mutation

Changing TEL11R to a unique end

Promoter

lac promoter

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pJJF144_SECGFP_sg2

Plasmid

#163865

Purpose

sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting GFP

Use

CRISPR

Expression

Worm

Promoter

R07E5.16 (U6)

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJJF152_Phsp_Cas9

Plasmid

#163862

Purpose

heat shock-induced expression of Cas9 in C. elegans

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

Cas9, C. elegans codon optimized with intron and SV40_NLS

Use

CRISPR

Expression

Worm

Promoter

Phsp-16.48

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJJF143_SECGFP_sg1

Plasmid

#163864

Purpose

sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting GFP

Use

CRISPR

Expression

Worm

Promoter

R07E5.16 (U6)

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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plIVMD163

Plasmid

#160935

Purpose

In vivo mRNA display construct for mCherry

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-mCherry-HIS tag, 3'UTR MS2 Stem Loop

Tags

HIS tag, 3'UTR MS2 Stem Loop

Expression

Yeast

Promoter

MET25

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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EMX1*_sgRNA

Plasmid

#100558

Purpose

Expresses EMX1* sgRNA. Target sequence: (G)GAGTCCGAGCAGAAGAAGAA. Same target sequence as #100555, except with an additional 5' G.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

EMX1 sgRNA

Promoter

U6

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3_zfh1_CP-candidate_luc+

Plasmid

#86391

Purpose

Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates responsiveness to the upstream enhancer is reflected by luciferase activity

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Insert

D. melanogaster zfh1 enhancer

Use

Luciferase

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only