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pET28a_6HT-TBP6.7(P51A)

Plasmid

#112728

Purpose

Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P51A)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

P51A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(Q54A)

Plasmid

#112730

Purpose

Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q54A)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

Q54A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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LLhT-pHG165a V52A

Plasmid

#90050

Purpose

Constitutive expression of a chimeric LacI:TreR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhT/V52A

Use

Tags

Expression

Bacterial

Mutation

V52A

Promoter

Iq

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhA-pHG165a Q55A

Plasmid

#90052

Purpose

Constitutive expression of a chimeric LacI:AscG transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhA/Q55A

Use

Tags

Expression

Bacterial

Mutation

Q55A

Promoter

Iq

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhC-pHG165a Q55I

Plasmid

#90053

Purpose

Constitutive expression of a chimeric LacI:CytR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhC/Q55I

Use

Tags

Expression

Bacterial

Mutation

Q55I

Promoter

Iq

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-ECC-Biosensor

Plasmid

#140832

Purpose

Encoding the CAG promoter based eccDNA biosensor

Depositor

Yonglun Luo

Article

Moller et al Nucleic Acids Res. 2018 Dec 14;46(22):e1

Insert

ECC-Biosensor

Use

Piggybac (pb) transposon

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pIRES-hrGFP II-TET1

Plasmid

#83568

Purpose

Expresses TET1 in mammalian cells

Depositor

Jean-Pierre Issa

Article

Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

Insert

Tet Methylcytosine Dioxygenase 1 (TET1 Human)

Use

Tags

3X FLAG tag and hr GFP II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCVhyg-H3.3WT-FLAG/HA

Plasmid

#128740

Purpose

Expression of histone H3.3WT-FLAG/HA

Depositor

Kai Ge

Article

Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607

Insert

H3.3 WT (H3-3A Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mutation

Promoter

MSCV

Available since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCVhyg-H3.3K4M-FLAG/HA

Plasmid

#128741

Purpose

Expression of histone H3.3K4M-FLAG/HA

Depositor

Kai Ge

Article

Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607

Insert

H3.3K4M (H3-3A Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mutation

K4M

Promoter

MSCV

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pRR-AR

Plasmid

#73045

Purpose

Constitutively expresses the androgen receptor, which is required for the activation of the promoter in pLAREG (Addgene plasmid #73045)

Depositor

David McMillen

Article

Mazumder et al Nucleic Acids Res. 2014 Aug;42(14):9514-

Insert

Androgen receptor (AR Human)

Use

Tags

Expression

Yeast

Mutation

please see depositor comments below

Promoter

glyceraldehyde phosphate dehydrogenase (GPD)

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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Cas9-sgRNA-A+B

Plasmid

#149370

Purpose

Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites

Depositor

Tarek Abbas

Article

Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

Inserts

Cas9
sgRNA-A
sgRNA-B
Puromycin resistance

Use

CRISPR

Tags

3xFLAG, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

Promoter

CBh, PGK, and U6

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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LLP786_SpyCatcher-EZH2-FL-CO

Plasmid

#211779

Purpose

SpyTag counterpart binding domain, SpyCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

aGCN4-KRAB

Use

Tags

3xTy1

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect KRAB e…

Promoter

pEF1a and pSV40

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP‐RNASEH1

Plasmid

#108699

Purpose

For expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cells

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

RNASEH1 (nuclear isoform) (RNASEH1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-dCasRx-METTL3-PURO

Plasmid

#174218

Purpose

Expresses dCasRx-METTL3 in mammalian cell

Depositor

Qi Xie

Article

Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

Insert

dCasRx-METTL3

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

pMSCV

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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AAV CMV-driven AcrIIC3-scaffold (2xBsmBI sites)

Plasmid

#120301

Purpose

AAV vector for expression of AcrIIC3 (no miR binding sites, control vector)

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIC3

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pKD068

Plasmid

#136471

Purpose

SSB-GFP IDL fusion expression plasmid

Depositor

James Keck

Article

Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

Insert

SSB-GFP (ssb E. coli)

Use

Tags

superfolder GFP

Expression

Bacterial

Mutation

superfolder GFP inserted between Phe148 and Ser149

Promoter

T7 promoter

Available since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-ABE7.10-P2A-GFP

Plasmid

#162509

Purpose

Express ABE7.10-P2A-GFP by CMV promoter in mammalian cells

Depositor

Yongsub Kim

Article

Shin et al Nucleic Acids Res. 2021 Feb 26;49(4):239

Insert

ABE7.10-P2A-GFP

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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KRAB-dCas9

Plasmid

#112195

Purpose

KRAB fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Insert

KRAB (ZNF10 Human)

Use

CRISPR

Tags

3XFLag-NLS-KRAB-dCas9-NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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PUC19_C_tag

Plasmid

#186659

Purpose

Empty donor plasmid for C-terminal tag of 3xFlag-3xHA.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

C-terminal 3xFlag-3xHA Tag

Use

Tags

3xFlag-3xHA

Expression

Insect

Mutation

Promoter

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Drosha

Plasmid

#62520

Purpose

wild-type human Drosha cDNA is subcloned into pEGFP-C1 vector between HindIII and KpnI sites

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha full length (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only

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