We narrowed to 8,862 results for: BLI;
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Plasmid#175474PurposeProtein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.DepositorInsertPRDX6 (PRDX6 Human)
UseTagsAviTag and His6-TEVExpressionBacterialMutationSee Depositor CommentsPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal
Plasmid#52694PurposeThis plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.DepositorInsertsCas9
Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
UseCRISPR; Toxoplasma gondiiTags3X FLAG and Nuclear localization signalExpressionMutationPromoterTgTUB1 and Toxoplasma U6Available sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GluA2-scFv-mNeon-KDEL
Plasmid#230057PurposeAAV plasmid that expresses a a single chain variable fragment (scFv) for anti- GluA2 with a C-terminal mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.DepositorInsertAnti-GluA2 single chain variable fragment (scFv)
UseAAVTagsER retrieval motif (KDEL) and mNeonExpressionMammalianMutationPromoterhuman synapsinAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
p5'Entry-lck-7.4kb
Plasmid#58891Purposeprovides zebrafish lck promoter (7.4 kb) for GateWay construction of Tol2 transgenesDepositorInsertzebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 7.4 kb
UseGateway 5' entry cloneTagsExpressionMutationmutated single nucleotide (T-->C, 1874th nucle…Promoterlymphocyte-specific protein tyrosine kinase (lck)…Available sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
HECTD1
Plasmid#32860PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHECTD1 (HECTD1 Human)
UseTagsHis (with TEV cleavage)ExpressionBacterialMutationamino acids 1879-1966 onlyPromoterAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBac_Dual_SNF2L-opt_p48
Plasmid#102243PurposeExpression of human codon optimized human SNF2L and pRbAp48 from pFastBac dualDepositorUseTagsExpressionInsectMutationcodon optimized for expressionPromoterPolyhedrin and p10Available sinceNov. 1, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
AICSDP-35:AAVS1-mEGFP
Plasmid#91565PurposeHomology arms and linker-mEGFP sequence for internal insertion of CAGGS-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFPDepositorInsertAAVS1 Homology Arms with CAGGS driven mEGFP (AAVS1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 7, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine
Plasmid#50472PurposeGs-coupled rM3D-IRES-mCitrine under the control of GFAP promoterDepositorUseAAVTagsHAExpressionMutationSee supplemental documents for DREADD mutationsPromoterGFAPAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)-BMTU UDG-6xHis
Plasmid#172197Purposeexpresses His-tagged uracil-DNA-glycosylase enzyme from marine bacterium BMTU 3346 for the purpose of cross contamination prevention in nucleic acid amplification reactionsDepositorInsertBMTU UDG
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pelBHisTEV
Plasmid#157741Purposeempty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sitesDepositorTypeEmpty backboneUseTagsPelB signal sequence + His10-tag + TEV protease c…ExpressionBacterialMutationPromoterT7lacAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-39:RAB5A-mEGFP
Plasmid#107579PurposeHomology arms and mEGFP-linker sequence for N-terminus tagging of human RAB5ADepositorInsertRAB5A Homology Arms with mEGFP-linker (RAB5A Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMay 9, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
p-dCas9-TET1-Hygro
Plasmid#104404Purposetransient expression of dCas9-TET1 fusion proteinDepositorInsertTET1 (TET1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
MECP2 in pENTR
Plasmid#216592Purposegateway cloning donor vector containing MECP2DepositorInsertMECP2 (MECP2 Human)
UseGateway entry plasmidTagsExpressionMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-dCas9-KRAB-gRNA-TRE-blast
Plasmid#201152PurposeLentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.DepositorInsertsdCas9-KRAB
gRNA-TRE
UseCRISPR and LentiviralTagsHAExpressionMutationgRNA sequence: TACGTTCTCTATCACTGATPromoterAvailable sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a
Plasmid#159908PurposeMutagenesis of Htr2aDepositorInsertHtr2a (Htr2a Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-Drp1
Plasmid#110946PurposeExpresses dynamin-like protein 1 fused to mTurquoise2DepositorInsertDynamin-1-like protein (DNM1L Human)
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE80L SpyTag-ELP-SpyTag
Plasmid#112634PurposeTwo SpyTags linked by an elastin-like polypeptide for hydrogel formationDepositorInsertSpyTag-ELP-SpyTag
UseTagsHis6ExpressionBacterialMutationcentral cysteinePromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOET5 CVB3-VLP
Plasmid#218146PurposeForms coxsackievirus 3-like particle in insect cells by expression of VP1–VP4 polyprotein (GeneID M33854.1) and 3CD protease (GeneID M33854.1).DepositorInsertsVP1–VP4
3CD
UseTagsExpressionInsectMutationPromoterCMV and PolyhedrinAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lamin A (G465D)-mRFP
Plasmid#124271PurposeLamin A mutant (G465D), C-terminally tagged with mRFP. Mutation associated with FPLD lipodystrophy.DepositorInsertLMNA (G465D) (LMNA Human)
UseTagsmRFPExpressionMammalianMutationLMNA (G465D)PromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pJCX3-ACTG2_R257C
Plasmid#228911PurposeExpresses mutant R257C smooth muscle actin gamma-2 in human cellsDepositorInsertActin gamma 2, smooth muscle (ACTG2 Human)
UseTagsHIS-FLAG-TEVExpressionBacterial and MammalianMutationR257CPromoterCMVAvailable sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJCX3-ACTG2_WT
Plasmid#228910PurposeExpresses wildtype smooth muscle actin gamma-2 in human cellsDepositorInsertActin gamma 2, smooth muscle (ACTG2 Human)
UseTagsHIS-FLAG-TEVExpressionBacterial and MammalianMutationWTPromoterCMVAvailable sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-FUS-G156E
Plasmid#181716PurposeGalactose inducible expression of FUS-G156EDepositorInsertFUS (FUS Human)
UseTagsExpressionYeastMutationG156EPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP Cas
Plasmid#50729Purposemammalian expression of GFP-Cas. Note-This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertCas (Bcar1 Rat)
UseTagsFLAG and GFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-HA-hM3D(Gq)-IRES-mCitrine
Plasmid#50470PurposeGq-coupled hM3D-IRES-mCitrine under the control of GFAP promoterDepositorInserthM3D(Gq)-IRES-mCitrine (CHRM3 Human)
UseAAVTagsHAExpressionMutationSee supplemental documents for DREADD mutationsPromoterGFAPAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
BRAF in pENTR
Plasmid#216605Purposegateway cloning donor vector containing BRAFDepositorInsertBRAF (BRAF Human)
UseGateway entry plasmidTagsExpressionMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b G12D
Plasmid#83131PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12D mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationG12DPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b
Plasmid#83129PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions)DepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-CAG-TVA-P2A-dTomato
Plasmid#177016PurposeFor expression of the avian TVA receptor in a cre-dependent mannerDepositorInsertTVA-P2A-dTomato (CD320 Coturnix japonica)
UseAAV and Cre/LoxTagsdTomatoExpressionMammalianMutationPromoterCAGGSAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
EEF1A2 in pENTR
Plasmid#216585Purposegateway cloning donor vector containing EEF1A2DepositorInsertEEF1A2 (EEF1A2 Human)
UseGateway entry plasmidTagsExpressionMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-UBC9
Plasmid#228949Purposeexpress a fragment of the wild-type human UBC9 (residues 2-158 of UniProt P63279 supplemented with the N-terminal thrombin-cleavable MGHHHHHHSSGLVPR|G- tag)DepositorInsertUBC9 (UBE2I Human)
UseTagsHis tag and thrombine cleavage site : MGHHHHHHSSG…ExpressionBacterialMutationPromoterT7-lacAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GluA1-scFv-mNeon-KDEL
Plasmid#230056PurposeAAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 tagged with mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.DepositorInsertAnti-GluA1 single chain variable fragment (scFv)
UseAAVTagsER retrieval motif (KDEL) and mNeonExpressionMammalianMutationPromoterhuman synapsinAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
psd44-G12QL
Plasmid#46825Purposeexpression of mutant G protein alpha 12 in mammalian cellsDepositorInsertGNA12 (GNA12 Human)
UseLentiviralTagsExpressionMammalianMutationQ231L (the mutation reduces GTPase activity resul…PromoterPGKAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4a
Plasmid#83166PurposeGateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions)DepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-117:POLR2A-mEGFP
Plasmid#164500PurposeHomology arms and mEGFP-linker sequence sequence for N-terminus tagging of human POLR2ADepositorInsertPOLR2A Homology Arms with mEGFP-linker (POLR2A Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceFeb. 17, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-Ef1a-Flex-Axon-GCaMP7b
Plasmid#135419PurposeCan be used to drive axon-enriched expression of GCaMP7b in the presence of Cre recombinase.DepositorInsertaxon-jGCaMP7b
UseAAVTags6xHis and GAP43 palmitoylation domainExpressionMammalianMutationPromoterEf1aAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
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Hs.KRAS4b G13D
Plasmid#83133PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G13D mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationG13DPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-HA-hM4D(Gi)-IRES-mCitrine
Plasmid#50471PurposeGi-coupled hM4D-IRES-mCitrine under the control of GFAP promoterDepositorInserthM4D(Gi)-IRES-mCitrine (CHRM4 Human)
UseAAVTagsHAExpressionMutationSee supplemental documents for DREADD mutationsPromoterGFAPAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine
Plasmid#50467PurposeGi-coupled hM4D-IRES-mCitrine under the control of CaMKIIa promoterDepositorUseAAVTagsHAExpressionMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGWB2-CYCD3;1
Plasmid#140750PurposeInducing plant cells to enter mitosisDepositorInsertCyclin D3;1 (CYCD3;1 Mustard Weed)
UseTagsExpressionPlantMutationPromoter35SAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
6xO/S luc
Plasmid#69445Purposedetection/assessment of OCT4 and SOX2 transcription factor activityDepositorInsertminimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
4IKP
Plasmid#60080PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertCARM1:JMC049-A06:C226836 (CARM1 Human)
UseBaculovirus expressionTags6xHisExpressionMutationSee commentsPromoterpolyhedrinAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
SARS-CoV-2 Spike-d18
Plasmid#164583PurposeA mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.DepositorInsertSARS-CoV-2 Spike (S SARS-CoV-2)
UseTagsExpressionMammalianMutationDeleted amino acids 1256-1273PromoterEF1aAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
JMC044-B12
Plasmid#58752Purposebacterial expression of PDB 4NW3DepositorInsertPDB:4NW3 (KMT2A Human)
UseTagsHisExpressionBacterialMutationBacterial expression for structure determination;…PromoterT7Available sinceAug. 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGWB660-CYCD3;1
Plasmid#140751PurposeInducing plant cells to enter mitosisDepositorInsertCyclin D3;1 (CYCD3;1 Mustard Weed)
UseTagsTagRFPExpressionPlantMutationPromoterCaMV 35 promoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b G12S
Plasmid#83144PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12S mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationG12SPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4a G12D
Plasmid#83168PurposeGateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G12D mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationG12DPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR1K18ms
Plasmid#72644PurposeGateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringaeDepositorTypeEmpty backboneUseSucrose counter-selection allelic exchange vectorTagsExpressionMutationPromoterAvailable sinceMarch 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAGGS-N2c(G)
Plasmid#73481PurposeN2c Glycoprotein expression vectorDepositorInsertN2c(G)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only