We narrowed to 18,983 results for: INO

1. pcDNA.5.1-FHS-TNRC6A-SD-S1332D/S1346D

   Plasmid

   #215906

   Purpose

   Expression of TNRC6A-silencing domain (SD) with S1332D/S1346D mutation

   Depositor

   Kumiko Ui-Tei

   Article

   Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   Flag-HA-SBP

   Expression

   Mammalian

   Mutation

   contain aa1224-1709, S1332D, S1346D

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA.5.1-FHS-TNRC6A-SD-S1332A/S1346A

   Plasmid

   #215907

   Purpose

   Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A mutation

   Depositor

   Kumiko Ui-Tei

   Article

   Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   Flag-HA-SBP

   Expression

   Mammalian

   Mutation

   contain aa1224-1709, S1332A, S1346A

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA.5.1-FHS-TNRC6A-SD-S1616D/S1691D

   Plasmid

   #215908

   Purpose

   Expression of TNRC6A-silencing domain (SD) with S1616D/S1691D mutation

   Depositor

   Kumiko Ui-Tei

   Article

   Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   Flag-HA-SBP

   Expression

   Mammalian

   Mutation

   contain aa1224-1709, S1616D, S1691D

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBa.FRB-3myc-KIF13AWLDLE tail 361-1749

   Plasmid

   #224412

   Purpose

   Mammalian expression of KIF 13a WLDLE

   Depositor

   Marvin Bentley

   Article

   Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

   Insert

   KIF 13a WLDLE (Kif13a Mouse)
   

   Use

   Tags

   FRB-3myc

   Expression

   Mammalian

   Mutation

   WLDLE

   Promoter

   Chicken Beta actin

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. gRNA_SOX2_HDR

   Plasmid

   #163752

   Purpose

   gRNA expression vector to target the SOX2 stop codon for HDR gene tagging in human cells.

   Depositor

   Jennifer Mitchell

   Article

   Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

   Insert

   gRNA_SOX2 (SOX2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBa.FRB-3myc-KIF13AS1371A tail 361-1749

   Plasmid

   #224411

   Purpose

   Mammalian expression of KIF 13a S1371A

   Depositor

   Marvin Bentley

   Article

   Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

   Insert

   KIF 13a S1371A (Kif13a Mouse)
   

   Use

   Tags

   FRB-3myc

   Expression

   Mammalian

   Mutation

   S1371A

   Promoter

   Chicken Beta actin

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. L2117-FKBP-GPA-114-RH-CyOFP1

   Plasmid

   #222884

   Purpose

   Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, RH linker, CyOFP1 protein.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FKBP-GPA-20GS-NanoLuc114-RH-CyOFP1
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. L2116-FRB-GPA-114-RH-CyOFP1

   Plasmid

   #222883

   Purpose

   Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, RH linker, CyOFP1 protein.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-20GS-NanoLuc114-RH-CyOFP1
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHR-CD80-CLIP

   Plasmid

   #223590

   Purpose

   Lentiviral expression of human CD80 with CLIP tag in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

   Insert

   CD80 (CD80 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   CLIP

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHR-SNAP-CD80

    Plasmid

    #223588

    Purpose

    Lentiviral expression of human CD80 with SNAP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CD80 (CD80 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    SNAP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. L2379-FKBP-GPA(V84R)-20-11S

    Plasmid

    #222901

    Purpose

    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FKBP-GPA(V84R)-20GS-NanoLuc11S
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    V84R in GPA

    Promoter

    CMV

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. L2380-FKBP-GPA(V84R)-20-114

    Plasmid

    #222900

    Purpose

    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FKBP-GPA(V84R)-20GS-NanoLuc114
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    V84R in GPA

    Promoter

    CMV

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. L2378-FRB-GPA(V84R)-20-11S

    Plasmid

    #222899

    Purpose

    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA(V84R)-20GS-NanoLuc11S
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    V84R in GPA

    Promoter

    CMV

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. L2377-FRB-GPA(V84R)-20-114

    Plasmid

    #222898

    Purpose

    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA(V84R)-20GS-NanoLuc114
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    V84R in GPA

    Promoter

    CMV

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. L2085-WT-GPA-NLuc-Biosensor-Retrovirus-TD135

    Plasmid

    #222875

    Purpose

    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). WT Glycophorin A (GPA) scaffold.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA(WT)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTFG011_Spike_Parental_D614G_3xFlag

    Plasmid

    #191571

    Purpose

    SARS-CoV-2 Spike protein under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. For expression and pseudotyping lentiviral particles.

    Depositor

    Joshua Leonard

    Article

    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

    Insert

    SARS-CoV-2 Spike Protein (S Synthetic)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    D614G mutation. Last 19 amino acids truncated.

    Promoter

    CMV

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. L2132-FKBP-GPA-114-5-CyOFP1

    Plasmid

    #222891

    Purpose

    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FKBP-GPA-20GS-NanoLuc114-5GS-CyOFP1
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. L2127-FRB-GPA-114-20-CyOFP1

    Plasmid

    #222886

    Purpose

    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-20GS-NanoLuc114-20GS-CyOFP1
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. L2129-FRB-GPA-114-5-CyOFP1

    Plasmid

    #222888

    Purpose

    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-20GS-NanoLuc114-5GS-CyOFP1
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. L2128-FRB-GPA-114-10-CyOFP1

    Plasmid

    #222887

    Purpose

    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-20GS-NanoLuc114-10GS-CyOFP1
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. L2131-FKBP-GPA-114-10-CyOFP1

    Plasmid

    #222890

    Purpose

    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FKBP-GPA-20GS-NanoLuc114-10GS-CyOFP1
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. L2130-FKBP-GPA-114-20-CyOFP1

    Plasmid

    #222889

    Purpose

    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FKBP-GPA-20GS-NanoLuc114-20GS-CyOFP1
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-Myc-FOXA3

    Plasmid

    #219395

    Purpose

    Express FOXA3 in mammalian cells

    Depositor

    Yoichi Furukawa

    Article

    Nakagawa et al Commun Biol. 2024 Apr 29;7(1):510. doi:

    Insert

    FOXA3 (FOXA3 Human)
    

    Use

    Tags

    N terminal Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AA054

    Plasmid

    #215932

    Purpose

    Fragmid fragment: (guide cassette) guide expression; reverse orientation

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    rev{U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v3}
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AA102

    Plasmid

    #215935

    Purpose

    Fragmid fragment: (guide cassette) guide expression; reverse orientation; enables iBar UMIs

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    rev{U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v5}
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AA107

    Plasmid

    #215936

    Purpose

    Fragmid fragment: (guide cassette) guide expression; enables iBar UMIs

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v5
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV2_hSyn_NES-Caprola_05-mEGFP_WRPE-SV40

    Plasmid

    #194689

    Purpose

    hSyn1 driven expression of the calcium recorder Caprola_05 fused to mEGFP for neuronal expression through AAV transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_05-mEGFP
    

    Use

    AAV

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    hSyn1

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTol2_elavl3(HuC)_NES-Caprola_on-mEGFP

    Plasmid

    #194697

    Purpose

    HuC driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression in zebrafish

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_on-mEGFP
    

    Use

    Zebrafish expression

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    elavl3(HuC)

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTol2_elavl3(HuC)_NES-Caprola_off-mEGFP

    Plasmid

    #194698

    Purpose

    HuC driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression in zebrafish

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_off-mEGFP
    

    Use

    Zebrafish expression

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    elavl3(HuC)

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKLV2-U6gRNA5(RUNX3(13))-PGKpuro2ABFP-W

    Plasmid

    #208552

    Purpose

    Lentiviral vector expressing gRNA targeting human RUNX3

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    RUNX3(13) (RUNX3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pKLV2-U6gRNA5(RUNX3(12))-PGKpuro2ABFP-W

    Plasmid

    #208551

    Purpose

    Lentiviral vector expressing gRNA targeting human RUNX3

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    RUNX3(12) (RUNX3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pKLV2-U6gRNA5(Control(1))-PGKpuro2AmCherry-W

    Plasmid

    #210612

    Purpose

    Lentiviral vector expressing gRNA targeting a control region at the CXCR4 locus

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    Control(1)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pKLV2-U6gRNA5(RIC8A(16))-PGKpuro2ABFP-W

    Plasmid

    #200491

    Purpose

    Lentiviral vector expressing gRNA targeting human RIC8A

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    RIC8A(16) (RIC8A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pKLV2-U6gRNA5(RIC8A(15))-PGKpuro2ABFP-W

    Plasmid

    #200490

    Purpose

    Lentiviral vector expressing gRNA targeting human RIC8A

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    RIC8A(15) (RIC8A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pKLV2-U6gRNA5(Control(2))-PGKpuro2AmCherry-W

    Plasmid

    #210613

    Purpose

    Lentiviral vector expressing gRNA targeting a control region at the CXCR4 locus

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    Control(2)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRAW-460

    Plasmid

    #200216

    Purpose

    Strep-TEV-PaCas1 + PaCas2/3 (Cas2 R55E, N56D)

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    PaCas1, PaCas2-3
    

    Use

    Tags

    Strep II-TEV on Cas1

    Expression

    Bacterial

    Mutation

    Cas2 R55E-N56D mutations

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRAW-461

    Plasmid

    #200217

    Purpose

    Strep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E)

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    PaCas1, PaCas2-3
    

    Use

    Tags

    Strep II-TEV on Cas1

    Expression

    Bacterial

    Mutation

    Cas2 K11D-R12E mutations

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRAW-462

    Plasmid

    #200218

    Purpose

    Strep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E, R55E, N56D)

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    PaCas1, PaCas2-3
    

    Use

    Tags

    Strep II-TEV on Cas1

    Expression

    Bacterial

    Mutation

    Cas2 K11D-R12E, R55E-N56D mutations

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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39. P15-pZE-SUMO-PiTTA

    Plasmid

    #204630

    Purpose

    ColE1 ori, KanR, TetR, Tet promoter with a codon optimized piTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.

    Depositor

    Aditya Kunjapur

    Article

    Jones et al Commun Biol. 2023 Sep 11;6(1):929. doi:

    Insert

    His-SUMO-TEV-BuTTA
    

    Use

    Synthetic Biology

    Tags

    His Tag; SUMO Tag; TEV Protease Site

    Expression

    Bacterial

    Mutation

    Promoter

    PLtetO-1 promoter

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. P17-pZE-SUMO-CsTTA

    Plasmid

    #204631

    Purpose

    ColE1 ori, KanR, TetR, Tet promoter with a codon optimized csTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.

    Depositor

    Aditya Kunjapur

    Article

    Jones et al Commun Biol. 2023 Sep 11;6(1):929. doi:

    Insert

    His-SUMO-TEV-CsTTA
    

    Use

    Synthetic Biology

    Tags

    His Tag; SUMO Tag; TEV Protease Site

    Expression

    Bacterial

    Mutation

    Promoter

    PLtetO-1 promoter

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. P18-pZE-SUMO-BuTTA

    Plasmid

    #204632

    Purpose

    ColE1 ori, KanR, TetR, Tet promoter with a codon optimized buTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.

    Depositor

    Aditya Kunjapur

    Article

    Jones et al Commun Biol. 2023 Sep 11;6(1):929. doi:

    Insert

    His-SUMO-TEV-BuTTA
    

    Use

    Synthetic Biology

    Tags

    His Tag; SUMO Tag; TEV Protease Site

    Expression

    Bacterial

    Mutation

    Promoter

    PLtetO-1 promoter

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. P24-pZE-SUMO-KaTTA

    Plasmid

    #204633

    Purpose

    ColE1 ori, KanR, TetR, Tet promoter with a codon optimized kaTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.

    Depositor

    Aditya Kunjapur

    Article

    Jones et al Commun Biol. 2023 Sep 11;6(1):929. doi:

    Insert

    His-SUMO-TEV-KaTTA
    

    Use

    Synthetic Biology

    Tags

    His Tag; SUMO Tag; TEV Protease Site

    Expression

    Bacterial

    Mutation

    Promoter

    PLtetO-1 promoter

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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43. pcDNA4-Xpress-GBF1(E794K)

    Plasmid

    #206418

    Purpose

    Mammalian expression of hGBF1(E794K)

    Depositor

    Kazuhisa Nakayama

    Article

    Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

    Insert

    GBF1 (GBF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E794K

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pcS-RDG-C1C2

    Plasmid

    #200163

    Purpose

    This plasmid encodes non-binder control monobody (RDG) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.

    Depositor

    Masako Harada

    Article

    Komuro et al Bioengineering (Basel). 2022 Jan 29;9(2)

    Inserts

    RDG-C1C2
    C1C2 domain of lactadherin
    

    Use

    Tags

    HA and HIS

    Expression

    Mammalian

    Mutation

    sequence adopted from doi: 10.1371/journal.pone.0…

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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45. pKUNOC-ErbB1(M721)-mScarlet

    Plasmid

    #197364

    Purpose

    Encoding human ErbB1 mutant tagged with mScarlet.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    EGFR mutant (EGFR Human)
    

    Use

    Tags

    Lysine 721 to Methionine and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pPBpuro-ErbB1(F992)

    Plasmid

    #197365

    Purpose

    Encoding human ErbB1 mutant.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    EGFR mutant (EGFR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Tyrosine 992 to Phenylalanine

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. SVA-lncRNA AK057321 shRNA 1S scramble

    Plasmid

    #202837

    Purpose

    Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 1S

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    SVA-lncRNA AK057321 shRNA 1S scramble control
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    6

    Available since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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48. SVA-lncRNA AK057321 shRNA 2S scramble

    Plasmid

    #202839

    Purpose

    Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 2S

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    SVA-lncRNA AK057321 shRNA 2S scramble control
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. pGL3-luciferase-reporter-POFUT2-SVA

    Plasmid

    #202831

    Purpose

    pGL3 luciferase reporter with POFUT2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    POFUT2 intronic SVA
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pGL3-luciferase-reporter-HTT-SVA

    Plasmid

    #202833

    Purpose

    pGL3 luciferase reporter with HTT SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    HTT intronic SVA
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge