We narrowed to 15,045 results for: NOL

1. pMM0652

   Plasmid

   #129013

   Purpose

   Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effects

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-KanR-ColE1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSTAP-seq_fly_spike-in

   Plasmid

   #125151

   Purpose

   Vector to measure CP candidates (spike-in) expression by determining the abundance of transcripts originating from the candidate by a D.pse enhancer in fly cells

   Depositor

   Alexander Stark

   Article

   Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

   Insert

   D. pseudoobscura peak #47 enhancer
   

   Use

   Stap-seq screening vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBT271.361

   Plasmid

   #127846

   Purpose

   clone A3A-W98Y CBEs by SapI Golden Gate

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   A3A-W98Y
   

   Use

   Tags

   Expression

   Mutation

   W98Y

   Promoter

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. YSBE420:pNEO_CAC1_Vn_HOG1 terminator

   Plasmid

   #122701

   Purpose

   Plasmid for tagging proteins in Cryptococcus neoformans; N-terminal fragment of Venus protein

   Depositor

   Yong-Sun Bahn

   Article

   So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

   Insert

   N-terminal fragment of Venus protein
   

   Use

   Tagging proteins in cryptococcus neoformans

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGS-BacA-21222-FLP-3xFLAG

   Plasmid

   #109165

   Purpose

   Encodes yeast full-length Flp with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

   Depositor

   Monika Golas

   Article

   Jensen et al Protein J. 2017 Aug;36(4):332-342. doi:

   Insert

   Flp
   

   Use

   Tags

   3xFLAG

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. YSBE418:pNAT_ACA1_Vc_HOG1 terminator

   Plasmid

   #122700

   Purpose

   Plasmid for tagging proteins in Cryptococcus neoformans; C-terminal fragment of Venus protein

   Depositor

   Yong-Sun Bahn

   Article

   So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

   Insert

   C-terminal fragment of Venus protein
   

   Use

   Tagging proteins in cryptococcus neoformans

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pOCC111

   Plasmid

   #118906

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal sortase site (LPETGg) and HIS6

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-LPETGg-HIS6-stop-HindIII cassette
   

   Use

   Tags

   LPETGg, HIS6

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. YORWΔ22Ω2

   Plasmid

   #101705

   Purpose

   GoldenBraid vector pDGB2Ω2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR loci

   Depositor

   Elena Caro Bernat

   Article

   Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

   Type

   Empty backbone

   Use

   Yeast expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. YORWΔ22Ω1

   Plasmid

   #101706

   Purpose

   GoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR loci

   Depositor

   Elena Caro Bernat

   Article

   Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

   Type

   Empty backbone

   Use

   Yeast expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. YORWΔ22α2

    Plasmid

    #101707

    Purpose

    GoldenBraid vector pDGB2α2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR loci

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Type

    Empty backbone

    Use

    Yeast expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

11. YORWΔ22α1

    Plasmid

    #101708

    Purpose

    GoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR loci

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Type

    Empty backbone

    Use

    Yeast expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. YPRCΔ15Ω2

    Plasmid

    #101709

    Purpose

    GoldenBraid vector pDGB2Ω2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Type

    Empty backbone

    Use

    Yeast expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. YPRCΔ15α2

    Plasmid

    #101711

    Purpose

    GoldenBraid vector pDGB2α2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Type

    Empty backbone

    Use

    Yeast expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pUPD-MAM33MTS (12)

    Plasmid

    #101693

    Purpose

    MAM33MTS in domestication vector

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Insert

    CCAT-[MTS from S. cerevisiae Superoxide dismutase [Mn] (YHR008C)from S. cerevisiae Mitochondrial acidic protein (YIL070C)]-AATG
    

    Use

    Yeast expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMB1-spect-PthrC3_25-eGFP

    Plasmid

    #107413

    Purpose

    This plasmid contain promoter PthrC3_25, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

    Depositor

    Kang Zhou

    Article

    Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PthrC3_25

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMB1-spect-PthrC3_7-eGFP

    Plasmid

    #107412

    Purpose

    This plasmid contain promoter PthrC3_7, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

    Depositor

    Kang Zhou

    Article

    Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PthrC3_7

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCfB6637

    Plasmid

    #106164

    Purpose

    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6681 into Yarrowia lipolytica chromosomal location IntE_3, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    unknown
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET15b+R2(L171C)

    Plasmid

    #108239

    Purpose

    Expresses PdR-PCNA2(L171C) fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA2(L171C) fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    PCNA2,L171C

    Promoter

    T7 promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET15T+1X

    Plasmid

    #108188

    Purpose

    PCNA1-PdX fusion protein

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PCNA1-PdX fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    C73S/C85S (PdX)

    Promoter

    T7 promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEHIShqprtR161Q

    Plasmid

    #92413

    Purpose

    hQPRTase harboring R161Q mutation

    Depositor

    James Naismith, Huanting Liu

    Article

    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

    Insert

    hQPRTase (QPRT Human)
    

    Use

    Tags

    TEV protease cleavable 6xHis

    Expression

    Bacterial

    Mutation

    R161Q

    Promoter

    T7

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCML 221

    Plasmid

    #98587

    Purpose

    pMS2-CYFP. Expresses MS2 protein-CYFP fusion in E. coli for TriFC assay (kanR).

    Depositor

    Lydia Contreras

    Article

    Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

    Insert

    MS2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    pLacO

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEHIAhqprtR161G

    Plasmid

    #92412

    Purpose

    hQPRTase harbouring R161G mutation

    Depositor

    James Naismith, Huanting Liu

    Article

    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

    Insert

    hQPRTase (QPRT Human)
    

    Use

    Tags

    TEV protease cleavable 6xHis

    Expression

    Bacterial

    Mutation

    R161G

    Promoter

    T7

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEHIShqprtR102Q

    Plasmid

    #92407

    Purpose

    hQPRTase harboring R102Q mutation

    Depositor

    James Naismith, Huanting Liu

    Article

    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

    Insert

    hQPRTase (QPRT Human)
    

    Use

    Tags

    TEV protease cleavable 6xHis

    Expression

    Bacterial

    Mutation

    R102Q

    Promoter

    T7

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEHIShqprtK171S

    Plasmid

    #92404

    Purpose

    hQPRTase harbouring K171S mutation

    Depositor

    James Naismith, Huanting Liu

    Article

    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

    Insert

    hQPRTase (QPRT Human)
    

    Use

    Tags

    TEV protease cleavable 6xHis

    Expression

    Bacterial

    Mutation

    K171S

    Promoter

    T7

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEHIShqprtR138Q

    Plasmid

    #92411

    Purpose

    hQPRTase harbouring R138Q mutation

    Depositor

    James Naismith, Huanting Liu

    Article

    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

    Insert

    hQPRTase (QPRT Human)
    

    Use

    Tags

    TEV protease cleavable 6xHis

    Expression

    Bacterial

    Mutation

    R138Q

    Promoter

    T7

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEHIShqprtR102A

    Plasmid

    #92405

    Purpose

    hQPRTASE harbouring R102A mutation

    Depositor

    James Naismith, Huanting Liu

    Article

    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

    Insert

    hQPRTase (QPRT Human)
    

    Use

    Tags

    TEV protease cleavable 6xHis

    Expression

    Bacterial

    Mutation

    R102A

    Promoter

    T7

    Available since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBW1944_ hU6-PV4-loxP-BbsI-ENTRY-gRNA

    Plasmid

    #87572

    Purpose

    PV4 gRNA entry vector for CRISPR decoder

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBW1943_ hU6-PV3-FRT-loxP-BbsI-ENTRY-gRNA

    Plasmid

    #89056

    Purpose

    PV3 gRNA entry vector for CRISPR decoder

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pILGFPB6

    Plasmid

    #83557

    Purpose

    Used to evaluate the expression output of GAL10 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    GAL10 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. const #7-RFP

    Plasmid

    #81081

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Paul Adams, Jay Keasling

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Tags

    Expression

    Mutation

    Promoter

    Constitutive Promoter - BBa_J23109

    Available since

    Aug. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pconst #6-RFP

    Plasmid

    #81080

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Paul Adams, Jay Keasling

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Tags

    Expression

    Mutation

    Promoter

    Constitutive Promoter - BBa_J23117

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. ARK1a

    Plasmid

    #81016

    Purpose

    E.coli BW25113 + JBEI-12056 (pBbA5c-MevTo-BBa1002-pTrc-MKco-PMKco, JBx_041105) + JBEI-9348 (pTrc99a-PMDsc-NudB, JBx_039365)

    Depositor

    Taek Soon Lee

    Article

    Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

    Inserts

    MevTo-BBa1002-pTrc-Mkco-PMKco
    PMD-NudB
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    LacUV5 and Trc promoter

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSTARR-seq_fly-x16

    Plasmid

    #71505

    Purpose

    flySTARR-seq screening vector with x16 core promoter

    Depositor

    Alexander Stark

    Article

    Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

    Type

    Empty backbone

    Use

    Flystarr-seq screening vector

    Tags

    sgGFP (SuperGlo, Qbiogene, Inc)

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGL3-RpS12-Gateway

    Plasmid

    #71508

    Purpose

    flySTARR-seq validation vector with Gateway cassette downstream of the firefly luciferase gene and the RpS12 core promoter

    Depositor

    Alexander Stark

    Article

    Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

    Type

    Empty backbone

    Use

    Flystarr-seq validation vector

    Tags

    luciferase

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSTARR-seq_fly-eEF1delta

    Plasmid

    #71501

    Purpose

    flySTARR-seq screening vector with eEF1delta core promoter

    Depositor

    Alexander Stark

    Article

    Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

    Type

    Empty backbone

    Use

    Flystarr-seq screening vector

    Tags

    sgGFP (SuperGlo, Qbiogene, Inc)

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCellFree_G03 TCEB3B

    Plasmid

    #67117

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TCEB3B (ELOA2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

37. pCellFree_G03 ZFYVE9

    Plasmid

    #67134

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    ZFYVE9 (ZFYVE9 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    F1359I

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pET11+PCNA3-CYP119

    Plasmid

    #66136

    Purpose

    Expresses the wild type of CYP119 fused to Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Sulfolobus solfataricus PCNA3

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET11+PCNA3-CYP119(T214V)

    Plasmid

    #66137

    Purpose

    Expresses the T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Sulfolobus solfataricus PCNA3

    Expression

    Bacterial

    Mutation

    T214V

    Promoter

    T7 promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET15b+PCNA3 T180C

    Plasmid

    #66181

    Purpose

    Expresses the T180C mutant of Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 3
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    T180C

    Promoter

    T7 promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET15b+PCNA3 R112C

    Plasmid

    #66180

    Purpose

    Expresses the R112C mutant of Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 3
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C

    Promoter

    T7 promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pET15b+PCNA2 E105C

    Plasmid

    #66176

    Purpose

    Expresses the E105C mutant of Sulfolobus solfataricus PCNA2

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 2
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E105C

    Promoter

    T7 promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET11+PCNA3-CYP119(D77R/T214V)

    Plasmid

    #66138

    Purpose

    Expresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Sulfolobus solfataricus PCNA3

    Expression

    Bacterial

    Mutation

    D77R, T214V

    Promoter

    T7 promoter

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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44. pBGSA FPR1 Q258A

    Plasmid

    #66724

    Purpose

    Constitutive expression of FPR1 Q258A in mammalian cells.

    Depositor

    Heini Miettinen

    Article

    Miettinen et al J Immunol. 1997 Oct 15;159(8):4045-54.

    Insert

    FPR1 (haplotype 3) (FPR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q258A

    Promoter

    SR-alpha

    Available since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

45. pASKt15C+SrtA5woH

    Plasmid

    #65019

    Purpose

    Expresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, P94R, D160N, D165A, K19…

    Promoter

    tetracycline promoter/operator

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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46. pTH1-SaSrtA-KQ

    Plasmid

    #64982

    Purpose

    Expresses the E105K/E108Q mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E105K, E108Q

    Promoter

    Available since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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47. Gal4pBS13-Pleum

    Plasmid

    #60332

    Purpose

    Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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48. ROZA YF

    Plasmid

    #64197

    Purpose

    Fluorescent Reporter for ZAP-70 tyrosine kinase activity-control YF mutant

    Depositor

    Annemarie Lellouch

    Article

    Randriamampita et al PLoS One. 2008 Jan 30;3(1):e1521. doi: 1

    Insert

    ROZA YF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    tyrosine phosphorylatin site mutated to phenylala…

    Promoter

    CMV

    Available since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

49. Gal4pBS2-Pleum

    Plasmid

    #59992

    Purpose

    Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 11, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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50. UASGAL-CU1-Pleum

    Plasmid

    #60331

    Purpose

    Contains a promoter comprised of derivatives from native promoters galactose, cyc and leucine.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge