We narrowed to 17,948 results for: COMP

1. pCIneo-RLuc-HsAsb9-3UTR_X

   Plasmid

   #147949

   Purpose

   Mammalian Expression of HsAsb9-3UTR

   Depositor

   Elisa Izaurralde

   Article

   Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

   Insert

   HsAsb9-3UTR (ASB9 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1-MS2-HA-HsSmg5-Q123E_T

   Plasmid

   #147555

   Purpose

   Mammalian Expression of HsSmg5-Q123E

   Depositor

   Elisa Izaurralde

   Article

   Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

   Insert

   HsSmg5-Q123E (SMG5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pT7-EGFP-C1-HsUPF2-siRNAres_I

   Plasmid

   #146573

   Purpose

   Mammalian Expression of HsUPF2-siRNAres

   Depositor

   Elisa Izaurralde

   Article

   Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

   Insert

   HsUPF2-siRNAres (UPF2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAc5.1C-Fluc-DmHunchback_W

   Plasmid

   #147818

   Purpose

   Insect Expression of DmHunchback-3UTR

   Depositor

   Elisa Izaurralde

   Article

   Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

   Insert

   DmHunchback-3UTR (hb Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGADT7-AP-1 σ1B

   Plasmid

   #198335

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1B fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

   Insert

   AP-1 σ1B
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   Expresses a LELQMNRRY nonapeptide after the last …

   Promoter

   ADH1

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGADT7-AP-1 σ1A

   Plasmid

   #198334

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1A fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

   Insert

   AP-1 σ1A
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   Expresses a LELQMNRRY nonapeptide after the last …

   Promoter

   ADH1

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGFP-C2-tepsin

   Plasmid

   #196707

   Purpose

   Expresses GFP-tepsin in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

   Insert

   tepsin (TEPSIN Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   silent substitution in codon 199 to eliminate int…

   Promoter

   CMV

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-hSyn-ASAP3ER

   Plasmid

   #195358

   Purpose

   ASAP3ER under control of human synapsin promoter

   Depositor

   Brenda Bloodgood

   Article

   Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

   Insert

   ASAP3ER
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-2X(CMV-eBFP2-3XNLS-pA)

   Plasmid

   #195867

   Purpose

   to express nuclear localized eBFP2

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

   Insert

   eBFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMET7-GAG-eDHFR

    Plasmid

    #194635

    Purpose

    Mammalian expression of the eDHFR control bait in Virotrap

    Depositor

    Sven Eyckerman

    Article

    Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

    Insert

    p55 GAG
    

    Use

    Tags

    ecDHFR

    Expression

    Mammalian

    Mutation

    Promoter

    SRalpha

    Available since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FLAG-mVenusC-14-HP1b Chromo

    Plasmid

    #191398

    Purpose

    HP1b chromo domain fused to C-terminal part of split mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    FLAG-mVenusC-14-HP1b Chromo
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Flag-NLS-MaSat ZF-18-mVenusN

    Plasmid

    #191392

    Purpose

    Zinc finger fused to N-terminal part of split mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    Flag-NLS-MaSat ZF-18-mVenusN
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGAL1-VP1 (LEU2)

    Plasmid

    #182480

    Purpose

    Yeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains leucine auxotrophic marker (K. lactis LEU2).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Insert

    deltaVP1
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mRuby3 only VLP

    Plasmid

    #182429

    Purpose

    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and mRuby3 tagged with VP2C (GAL10 promoter). mRuby3 was codon-optimised for E. coli. Contains uracil marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-mRuby3
    deltaVP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENT-L3-eBra-L4

    Plasmid

    #186368

    Purpose

    Entry clone with ORF encoding Brachury cis-regulatory region flanked by Gateway recombination sequences

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    Brachury notochord enhancer
    

    Use

    Protein expression

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENT-L3-Titf-L4

    Plasmid

    #186367

    Purpose

    Entry clone with ORF encoding Titf1 cis-regulatory region flanked by Gateway recombination sequences

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    Titf endoderm enhancer
    

    Use

    Protein expression

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pENT-L3-pSnail-L5

    Plasmid

    #186365

    Purpose

    Entry clone with ORF encoding Ci-Snail cis-regulatory region flanked by Gateway recombination sequences

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    Snail regulatory sequence
    

    Use

    Protein expression

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Full-length WT talin1

    Plasmid

    #182992

    Purpose

    express FL WT talin1 in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    Talin1 WT (Tln1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Full-length P229S talin1

    Plasmid

    #182993

    Purpose

    express FL variant P229S talin1 in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    Talin1 P229S (Tln1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    P229S

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7-V5-SBP-C1-DmRoquin-ILLVtoEEEE_AE

    Plasmid

    #148636

    Purpose

    Mammalian Expression of DmRoquin-ILLVtoEEEE

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    DmRoquin-ILLVtoEEEE (roq Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pT7-V5-SBP-C1-HsRoquin1_AE

    Plasmid

    #148637

    Purpose

    Mammalian Expression of HsRoquin1

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    HsRoquin1 (RC3H1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pT7-V5-SBP-C1-HsRoquin1_501-1133_AE

    Plasmid

    #148638

    Purpose

    Mammalian Expression of HsRoquin1_501-1133

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    HsRoquin1_501-1133 (RC3H1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pT7-V5-SBP-C1-HsRoquin2_AE

    Plasmid

    #148639

    Purpose

    Mammalian Expression of HsRoquin2

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    HsRoquin2 (RC3H2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pT7-V5-SBP-C1-HsRoquin2_1-500_AE

    Plasmid

    #148640

    Purpose

    Mammalian Expression of HsRoquin2_1-500

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    HsRoquin2_1-500 (RC3H2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pT7-V5-SBP-C1-HsRoquin2_501-1191_AE

    Plasmid

    #148641

    Purpose

    Mammalian Expression of HsRoquin2_501-1191

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    HsRoquin2_501-1191 (RC3H2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pT7-V5-SBP-C1-HsRoquin_1-500_AE

    Plasmid

    #148642

    Purpose

    Mammalian Expression of HsRoquin1_1-500

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    HsRoquin1_1-500 (RC3H1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pT7-V5-SBP-C1-DmRoquin_AD

    Plasmid

    #148535

    Purpose

    Mammalian Expression of DmRoquin

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    DmRoquin (roq Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pT7-V5-SBP-C1-DmRoquin_1-500_AD

    Plasmid

    #148536

    Purpose

    Mammalian Expression of DmRoquin_1-500

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    DmRoquin_1-500 (roq Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pT7-V5-SBP-C1-DmRoquin_501-819_AD

    Plasmid

    #148537

    Purpose

    Mammalian Expression of DmRoquin_501-819

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    DmRoquin_501-819 (roq Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pT7-EGFP-C1-CrPV-Rluc-6xbsMS2-PolyA-Malat_AC

    Plasmid

    #148446

    Purpose

    Mammalian Expression of 6xbsMS2-PolyA-Malat

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    6xbsMS2-PolyA-Malat
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pT7-EGFP-C1-HCV-RLuc-6xbsMS2-polyA-Malat_AC

    Plasmid

    #148448

    Purpose

    Mammalian Expression of 6xbsMS2-polyA-Malat

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    6xbsMS2-polyA-Malat
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pnYC-NpM-HsSMG7opt_732-1091_V

    Plasmid

    #147769

    Purpose

    Bacterial Expression of HsSMG7opt_732-1091

    Depositor

    Elisa Izaurralde

    Article

    Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

    Insert

    HsSMG7opt_732-1091 (SMG7 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pYPQ131-RZ-Cas12j2

    Plasmid

    #189782

    Purpose

    Golden Gate entry vector to clone the 1st Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pYPQ132-RZ-Cas12j2

    Plasmid

    #189783

    Purpose

    Golden Gate entry vector to clone the 2nd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pYPQ134-RZ-Cas12j2

    Plasmid

    #189785

    Purpose

    Golden Gate entry vector to clone the 4th Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pENT-R4-bpFOG-L5

    Plasmid

    #186369

    Purpose

    Entry clone with ORF encoding Strong basal promoter of FOG flanked by Gateway recombination sequences

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    Basal promoter of the Friend Of Gata gene
    

    Use

    Protein expression

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pENT-L3-pFOGl-L5

    Plasmid

    #186364

    Purpose

    Entry clone with ORF encoding Ci-FOG cis-regulatory region flanked by Gateway recombination sequences

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    Friend Of Gata regulatory sequence
    

    Use

    Protein expression

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pYPQ131-RZ-Cas12j2

    Plasmid

    #173927

    Purpose

    Golden Gate entry vector; empty vector to clone the 1st Cas12j2 crRNA with ribozyme processing

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pYPQ132-RZ-Cas12j2

    Plasmid

    #173928

    Purpose

    Golden Gate entry vector; empty vector to clone the 2nd Cas12j2 crRNA with ribozyme processing

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pYPQ133-RZ-Cas12j2

    Plasmid

    #173929

    Purpose

    Golden Gate entry vector; empty vector to clone the 3rd Cas12j2 crRNA with ribozyme processing

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pYPQ134-RZ-Cas12j2

    Plasmid

    #173930

    Purpose

    Golden Gate entry vector; empty vector to clone the 4th Cas12j2 crRNA with ribozyme processing

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. FnRPR9-10

    Plasmid

    #182568

    Purpose

    Human fibronectin fragment, type-III repeats 9-10, R1374A, P1376A, R1379A mutant

    Depositor

    Timothy Springer

    Article

    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

    Insert

    Human fibronectin fragment, type-III repeats 9-10
    

    Use

    Tags

    His and TEV cleavage site after His tag

    Expression

    Bacterial

    Mutation

    R1374A, P1376A, R1379A

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. FnRPR8-10

    Plasmid

    #182570

    Purpose

    Human fibronectin fragment, type-III repeats 8-10, R1374A, P1376A, R1379A mutant

    Depositor

    Timothy Springer

    Article

    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

    Insert

    Human fibronectin fragment, type-III repeats 8-10
    

    Use

    Tags

    His and TEV cleavage site after His tag

    Expression

    Bacterial

    Mutation

    R1374A, P1376A, R1379A

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA

    Plasmid

    #186407

    Purpose

    Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined gene of interest under control of an AttB3/B5 recombined reg. sequence.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pET_His6-MBP-TEV-Cas6-RT-Cas1_WT

    Plasmid

    #186274

    Purpose

    Expresses Thiomicrospira Cas6-RT-Cas1 in E. coli

    Depositor

    Jennifer Doudna

    Article

    Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

    Insert

    Thiomicropira Cas6-RT-Cas1
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pDEST-pSP172BSSPE-pFOGc::RfA

    Plasmid

    #186397

    Purpose

    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of the pFOG ectodermal regulatory sequence.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pDEST-pSP172BSSPE-Swa1::RfA

    Plasmid

    #186396

    Purpose

    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of a regulatory sequence cloned in the Swa1 restriction site.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pENT-L3-a-element-L4

    Plasmid

    #186366

    Purpose

    Entry clone with ORF encoding Otx cis-regulatory region flanked by Gateway recombination sequences

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    a-element early Otx neural enhancer
    

    Use

    Protein expression

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pENT-L1-Kozak-Venus-stop-L2

    Plasmid

    #186360

    Purpose

    Entry clone with ORF encoding Venus fluorescent protein flanked by Gateway recombination sequences

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    Venus yellow fluorescent protein
    

    Use

    Expression of the venus fluorescent protein

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pPpT4- HTA1prom-TIR1-FLAG

    Plasmid

    #189725

    Purpose

    Enables expression of OsTIR1 from the HTA1 promoter, used to implement the AID system in Komagataella phaffii (original name pLLpLL001)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    HTA1

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge