We narrowed to 12,102 results for: LEA
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Plasmid#73209PurposeExpresses myc-dL5(E52D)-mCer3 fusion protein in endoplasmic reticulum of mammalian cells with an Igk-leader. (MBIC5, dL5**, FAP)DepositorInsertkappa-myc-dL5-2XG4S-mCer3-KDEL (MYC Synthetic, Human)
TagsThe FAP and mCerulean3 are fused with 2 copies of…ExpressionMammalianMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterCMVAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-Venus-173-N-ORAI1
Plasmid#87618PurposeN-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.DepositorInsertVenus173-N-ORAI1 (ORAI1 Synthetic, Human)
Tags6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…ExpressionMammalianPromoterCMVAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GST-BHMT-I/G
Plasmid#104442PurposeAutophagy cargo assayDepositorInsertBHMT (BHMT Human)
TagsGFP-myc GFP is from Aequorea victoria and GSTExpressionMammalianMutationN43A in GST (in a potential KFERQ-like sequence).…PromoterCMVAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SS-HA-Sun1L-KDEL
Plasmid#187016PurposeDisruption of LINC ComplexDepositorInsertSS-HA-Sun1L-KDEL
UseLentiviralAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY0738 pCAG-NLS-SscINT
Plasmid#179113PurposeSscINT expression vectorDepositorInsertpCAG-NLS-SscINT
UseCRISPRAvailable sinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY0739 pCAG-NLS-SacINT
Plasmid#179114PurposeSacINT expression vectorDepositorInsertpCAG-NLS-SacINT
UseCRISPRAvailable sinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDule2-3-nitroTyrosine (A7)
Plasmid#174079PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32H H70T D158H I159A L162RPromoterGlnRS (constitutive)Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBABE-Tau-cYFP
Plasmid#92205PurposeRetroviral overexpression vector for α-Syn/Tau bimolecular fluorescence complementation (BiFC)DepositorAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBABE-Tau-nYFP
Plasmid#92204PurposeRetroviral overexpression vector for Tau bimolecular fluorescence complementation (BiFC)DepositorAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-DroshaS300/302A
Plasmid#62530PurposeSerines 300 and 302 of Drosha are mutated to alaninesDepositorInserthuman Drosha with serines 300 and 302 changed alanines (DROSHA Human)
TagsGFPExpressionMammalianMutationchanged serines 300 and 302 to alaninesAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-U6-sgTel-CMV-puro-P2A-mCherry-TRF1
Plasmid#169449Purposeexpression of TRF1-mcherrry and sgRNA targeting telomeresDepositorInsertmCherry-TRF1
UseLentiviralExpressionMammalianAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET30a-Tn5
Plasmid#127917PurposeProkaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.DepositorPublicationInsertTn5
TagsMxe intein - Chitin-binding domainExpressionBacterialMutationE54K, L372PPromoterT7Available sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_HA1h
Plasmid#190473PurposeExpress the high-affinity histamine sensor GRAB_HA1h in neuronsDepositorInsertHistamine sensor GRAB_HA1h (high affinity)
UseAAVPromoterhSynAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN41
Plasmid#61344PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)DepositorInsertmCherry
TagsGS linker-AsLov2-biNLS9 and PKIt NESExpressionMammalianPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NLS-mCherry
Plasmid#231890PurposemCherry-tagged nuclear rupture markerDepositorInsertmCherry-SV40 NLS-mCherry
ExpressionMammalianAvailable sinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
2xFKBP-mCh-KDEL
Plasmid#173010PurposeFKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.DepositorInsert2xFKBP-mCh-KDEL
TagsmCherryExpressionMammalianPromoterChicken beta actin (CAG)Available sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 V5 mDispatched WT 2x HA
Plasmid#126410PurposeExpression of V5 Dispatched WT HA in mammalian cell lines.DepositorInsertm-Dispatched1 (Disp1 Mouse)
TagsHAExpressionMammalianMutationV5 iserted between A106 and A107Available sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPS1888
Plasmid#1360DepositorPublicationInsertNLS-EYFP
ExpressionYeastAvailable sinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDisplay-GRAB_OT1.0
Plasmid#185384PurposeExpresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in mammalian cellsDepositorInsertGPCR activation based oxytocin sensor GRAB_OT1.0
ExpressionMammalianPromoterCMVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTAL3
Plasmid#31034DepositorInsertTALEN-FokI nuclease backbone
TagsAcV5 and FokI nucleaseAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by