We narrowed to 2,936 results for: NAK

1. pHAGE2-TetOminiCMV-OSK

   Plasmid

   #136552

   Purpose

   Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Oct4-P2A-Sox2-T2A-Klf4 (Klf4, Sox2, Pou5f1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetO-miniCMV (dox-inducible)

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-nEFCas9

   Plasmid

   #87115

   Purpose

   SpCas9 expression AAV backbone plasmid under control of nEF promoter

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   nEF-Cas9
   

   Use

   AAV and CRISPR

   Tags

   HA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLL3.7 FLAG-YAP1-TEAD-P-H2B-mCherry

   Plasmid

   #128327

   Purpose

   Reporter to evaluate YAP1/TEAD-mediated gene transcription

   Depositor

   Yutaka Hata

   Article

   Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

   Inserts

   H2B mCherry
   YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-EF-FH-TAZ S89A-ires-blast

   Plasmid

   #52084

   Purpose

   Lentiviral expression vector for TAZ S89A mutant with N-terminal FLAG and His tags

   Depositor

   Yutaka Hata

   Article

   Yang et al Mol Cell Biol. 2014 Feb 18.

   Insert

   TAZ S89A (WWTR1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and His

   Expression

   Mammalian

   Mutation

   S89A

   Promoter

   EF1a

   Available since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRIP-SFFV-EGFP-NLS-FLAG- cGAS E225A/D227A

   Plasmid

   #127660

   Purpose

   Over-express catalytically inactive EGFP-NLS-FLAG-cGAS E225A/D227A

   Depositor

   Nicolas Manel

   Article

   Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

   Insert

   cGAS (CGAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   E225A/D227A

   Promoter

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. p2711-pHAGE2-Ef1aL-TAZ4SA-UBC-tagBFP-loxP

   Plasmid

   #216480

   Purpose

   lentiviral dual expression of TAZ4SA and tagBFP with loxP site for Cre excision

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Inserts

   TAZ4SA (WWTR1 Human)
   tagBFP
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mutation

   S66A, S89A, S117A, S311A

   Promoter

   EF1aL and UBC

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRIP-SFFV-EGFP-FLAG-cGAS E225A/D227A

   Plasmid

   #127662

   Purpose

   Over-express catalytically inactive EGFP-FLAG-cGAS E225A/D227A

   Depositor

   Nicolas Manel

   Article

   Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

   Insert

   cGAS (CGAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   E225A/D227A

   Promoter

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLEX307-mFzd5

   Plasmid

   #102867

   Purpose

   Expresses mouse Fzd5 in mammalian cells

   Depositor

   Henry Ho

   Article

   Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

   Insert

   Frizzled 5 (Fzd5 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   A to G substitution at nucleotide 699 of Fzd5 ORF…

   Promoter

   EF1A

   Available since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-U6-dgRNA-CAG-MPH

   Plasmid

   #106259

   Purpose

   Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone

   Depositor

   Juan Belmonte

   Article

   Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

   Insert

   MS2-P65-HSF1 (HSF1 Synthetic, Human)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1 Sox2 HM a

    Plasmid

    #26353

    Depositor

    Matthew Meyerson

    Article

    Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

    Insert

    Sox2 shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. p2710-pHAGE2-Ef1aL-wtYAP-UBC-tagBFP-loxP

    Plasmid

    #216479

    Purpose

    lentiviral dual expression of wtYAP (YAP1) and tagBFP with loxP site for Cre excision

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    wtYAP (YAP1) (YAP1 Human)
    tagBFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1aL and UBC

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-2dV-Camui (T305D/T306D)

    Plasmid

    #220368

    Purpose

    T305D/T306D phosphomimetic mutant of pCAG-2dV-Camui

    Depositor

    Ryohei Yasuda

    Article

    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

    Insert

    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T305D/T306D)-mEGFP(A206K) (Camk2a Rat, Synthetic)
    

    Use

    Tags

    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

    Expression

    Mammalian

    Mutation

    changed both Threonine 305 and 306 to aspartic ac…

    Promoter

    CAG

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-EF-FH-TAZ-ires-blast

    Plasmid

    #52083

    Purpose

    Lentiviral expression vector for TAZ with N-terminal FLAG and His tags

    Depositor

    Yutaka Hata

    Article

    Yang et al Mol Cell Biol. 2014 Feb 18.

    Insert

    TAZ (WWTR1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and His

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTRIP-CMV-mCherry-FLAG-cGAS E225A/D227A

    Plasmid

    #127657

    Purpose

    Over-express catalytically inactive mCherry-FLAG-cGAS E225A/D227A

    Depositor

    Nicolas Manel

    Article

    Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

    Insert

    cGAS (CGAS Human)
    

    Use

    Lentiviral

    Tags

    Flag and mCherry

    Expression

    Mutation

    E225A/D227A

    Promoter

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQCXIP-mCherry-Halo-YAP1

    Plasmid

    #128336

    Purpose

    Retrovirus construct to express mCherry-Halo-YAP1

    Depositor

    Yutaka Hata

    Article

    Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Retroviral

    Tags

    mCherry and Halo-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLEVI(408)-ColE-iLight-msfGFP

    Plasmid

    #170268

    Purpose

    Expresses LexA_DBD-iLight-msfGFP under J23116 promoter and mCherry under ColE promoter with a LexA408 operator in bacteria.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    iLight
    

    Use

    Tags

    msfGFP

    Expression

    Bacterial

    Mutation

    To design iLight for expression in bacteria, a fu…

    Promoter

    pJ23116

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1 Sox2 3H b

    Plasmid

    #26352

    Depositor

    Matthew Meyerson

    Article

    Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

    Insert

    Sox2 shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-mTubb3

    Plasmid

    #87116

    Purpose

    AAV backbone plasmid including GFP knock-in donor and Tubb3gRNA for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    U6-mTubb3sgRNA-GFP-EF1a-mCherryKASH-hGHpA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-1BPNLS-Cas9-1BPNLS

    Plasmid

    #87108

    Purpose

    BPNLS-Cas9-BPNLS expression plasmid

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    1BPNLS-Cas9-1BPNLS
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-2dV-Camui (T286A)

    Plasmid

    #220367

    Purpose

    T286A phosphodead mutant of pCAG-2dV-Camui

    Depositor

    Ryohei Yasuda

    Article

    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

    Insert

    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T286A)-mEGFP(A206K) (Camk2a Rat, Synthetic)
    

    Use

    Tags

    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

    Expression

    Mammalian

    Mutation

    changed Threonine 286 to Alanine

    Promoter

    CAG

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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