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pcDNA3.1_LifeAct-iBlueberry

Plasmid

#73176

Purpose

Expressing iBlueberry and mCherry in mammalian cells

Depositor

Xiaokun Shu

Article

Yu et al Protein Sci. 2016 Feb;25(2):308-15. doi:

Insert

LifeAct-iBlueberry

Expression

Mammalian

Mutation

mutation E177Q in iBlueberry

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pTRE_tau-LacZ::tTA-H100Y

Plasmid

#34927

Depositor

Mark Mayford

Article

Reijmers et al Science. 2007 Aug 31;317(5842):1230-3.

Inserts

tau (MAPT Bovine)
tTA

Use

Mouse Targeting

Tags

LacZ

Expression

Mammalian

Mutation

Made doxycycline insensitive by introduction of a…

Promoter

tetO

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-Lifeact-miRFP703

Plasmid

#178530

Purpose

Expresses Lifeact-miRFP703 in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

Lifeact-miRFP703

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis(TEV)-Drp1-StrepII

Plasmid

#174422

Purpose

Expresses human Drp1 Isoform 3 in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Tags

6xHis(TEV) and StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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GFPZFN1.4-B2H

Plasmid

#26791

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

GFP-ZFN1

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pMYCO1_SpdCas9_pmcDA1

Plasmid

#204615

Purpose

CRISPR cytosine deaminase replicative plasmid for Mycoplasma mycoides subsp. mycoides

Depositor

Pascal Sirand-Pugnet

Article

Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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GFPZFN2-B2H

Plasmid

#26792

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

GFP-ZFN2

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pCI-Neo-His-Pitaire-Myc

Plasmid

#26066

Depositor

Jonathon Pines

Article

Ko et al J Cell Sci. 2001 Jul . 114(Pt 14):2591-6

Insert

Pitaire (CDK12 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only
CROP_C9_Puro_HDAC2

Plasmid

#183298

Purpose

All-in-One CRISPRko system with a guide RNA that targets HDAC2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

HDAC2

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG vGlut1 GCaMP5G

Plasmid

#105008

Purpose

Presynaptic calcium sensor

Depositor

Franck Polleux

Article

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

SLC17A7

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTREX GPR68_NB2_2

Plasmid

#127691

Purpose

Plasmid contains a nanobody that directly interacts with the cytoplasmic surface of GPR68.

Depositor

Justin English, Bryan Roth

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

GPR68_NB2_2

Expression

Mammalian

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-PpU6P-sgRNA-L3L2

Plasmid

#113740

Purpose

Gateway entry vector containing attL3 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR; Gateway entry vector

Promoter

P. patens U6 promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-PpU6P-sgRNA-L1L4

Plasmid

#113736

Purpose

Gateway entry vector containing attL1 and attL4 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR; Gateway entry vector

Promoter

P. patens U6 promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 5HT2A_NB2_1

Plasmid

#127690

Purpose

Plasmid contains a positive allosteric modulating nanobody the binds to the cytoplasmic surface of 5HT2A.

Depositor

Justin English, Bryan Roth

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

5HT2A_NB2

Expression

Mammalian

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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35S-DsREDmonomer-NosT

Plasmid

#79183

Purpose

Transient expression vector for DsRED monomer

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Proc Natl Acad Sci U S A. 2010 Nov 9;107

Type

Empty backbone

Use

Reporter plasmid

Tags

DsREDmonomer

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLNC Wnt-3aHA

Plasmid

#18030

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-3a (Wnt3a Mouse)

Use

Retroviral

Tags

HA

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only

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pEGFP-Bik

Plasmid

#10952

Depositor

Bingliang Fang

Article

Kagawa et al Cancer Res. 2001 Apr 15. 61(8):3330-8.

Insert

human Bik gene (BIK Human)

Tags

GFP

Expression

Mammalian

Mutation

NA

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-C1

Plasmid

#54801

Purpose

Localization: C1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Type

Empty backbone

Tags

mCherry-miniSOG

Expression

Mammalian

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only
YFP-mDia1FH2deltaN

Plasmid

#25419

Depositor

Arthur Alberts

Article

Wallar et al Exp Cell Res. 2007 Feb 1. 313(3):560-71.

Insert

diaph1 (Diaph1 Mouse)

Tags

YFP A206K

Expression

Mammalian

Mutation

mDia1 FH2∆N2 sequence (encodes amino acids 771-11…

Available Since

July 20, 2010

Availability

Academic Institutions and Nonprofits only
3789 pmko-neo RNAi-GFP

Plasmid

#8881

Depositor

Stanley Korsmeyer

Article

Wang et al EMBO J. 2005 Jan 26. 24(2):368-81.

Insert

GFP

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only