We narrowed to 26,265 results for: STI

1. AICSDP-72:TFAM-mEGFP

   Plasmid

   #159745

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human TFAM

   Depositor

   Allen Institute for Cell Science

   Insert

   TFAM Homology Arms with linker-mEGFP (TFAM Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   Oct. 13, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pKERepPar

   Plasmid

   #211827

   Purpose

   Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Tol2 8xSTAR-sTomato-NLS. PGK-puro

   Plasmid

   #136261

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   stem cell Ascl2 reporter, 8 repeats
   

   Use

   Tol2 transposase

   Tags

   sTomato-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-CMV-eGFP-SV40p-BlasR

   Plasmid

   #228271

   Purpose

   pLenti-CMV-eGFP-SV40p-BlasR plasmid for ectopic expression of GFP

   Depositor

   Judy Lieberman

   Article

   Fontana et al Cell. 2024 Sep 2:S0092-8674(24)00898-5.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. hKv2.1-HA

   Plasmid

   #111525

   Purpose

   Expresses human WT KCNB1 in mammalian cells

   Depositor

   Federico Sesti

   Article

   Cotella et al J Neurosci. 2012 Mar 21;32(12):4133-44.

   Insert

   human Kv2.1 + HA (KCNB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-X1-Neo-GFP-ATL2

   Plasmid

   #109020

   Purpose

   stable lentiviral expression of cDNA

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   ATL2 (ATL2 Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EIF-1a promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pC0062 RanCas13 (B06) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115213

   Purpose

   Expresses RanCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   RanCas13
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p5E-0.349cldn15la

   Plasmid

   #125026

   Purpose

   Five prime entry vector containing 349bp cldn15la promoter from zebrafish. Drives expression in intestinal epithelial cells.

   Depositor

   John Rawls

   Article

   Murdoch et al PLoS Pathog. 2019 Mar 7;15(3):e1007381.

   Insert

   Claudin 15-like a (cldn15la Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. hKv2.1-pEGFP-N1

   Plasmid

   #111532

   Purpose

   Expresses human KCNB1 in mammalian cells for imaging

   Depositor

   Federico Sesti

   Article

   Yu et al Cell Death Dis. 2017 Apr 6;8(4):e2737. d

   Insert

   human Kv2.1 (KCNB1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMT-Hrp48-ADARcd-E488Q-V5

    Plasmid

    #139685

    Purpose

    Expresses the hyperactive form of the catalytic domain of the ADAR protein fused to HRP-48 for use in "TRIBE" assays

    Depositor

    Michael Rosbash

    Article

    Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

    Insert

    Hrp48, ADAR (Adar Fly)
    

    Use

    Tags

    V5

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p1A

    Plasmid

    #162692

    Purpose

    Expresses H. neapolitanus CbbLS and S. elongatus Prk. Rescues CCMB1 in M9 glycerol at 10% CO2

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    H. neapolitanus CbbLS and S. elongatus Prk
    

    Use

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pECIA2

    Plasmid

    #47032

    Purpose

    This is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.

    Depositor

    Chris Garcia

    Article

    Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

    Type

    Empty backbone

    Use

    Secreted expression in drosophila culture

    Tags

    Fc(human IgG1), HRV 3C Protease Cleavage site, He…

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein (Copper-Inducible)

    Available since

    Aug. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCRISPRi

    Plasmid

    #199808

    Purpose

    vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

    Depositor

    Steve Salipante

    Article

    Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

    Inserts

    dCas9
    sgRNA with GFP cassette
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV7.1_3xFLAG-WWC3

    Plasmid

    #172996

    Purpose

    constitutive expression of FLAG-tagged WWC3

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-WWC3 (WWC3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA-DAB2

    Plasmid

    #162690

    Purpose

    Expresses DabAB2 inorganic carbon transport complex

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    DabAB2 inorganic carbon transport complex
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LB901: pMVP/PB/Blast-DEST

    Plasmid

    #121873

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone w/ Blast selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)

    Plasmid

    #171129

    Purpose

    LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)

    Depositor

    Benjamin Kleinstiver

    Article

    Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

    Insert

    U6 promoter entry plasmid for LwaCas13a crRNA cloning
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX6P1-hPREP

    Plasmid

    #179387

    Purpose

    Express human prolyl oligopeptidase (tagged) in bacterial cells

    Depositor

    Christian Gruber

    Article

    Retzl et al J Nat Prod. 2020 Nov 25;83(11):3305-3314

    Insert

    human prolyl oligopeptidase (PREP Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    TAC

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFE-Reu-FI-prk

    Plasmid

    #162696

    Purpose

    Expresses R. eutropha Form IC rubisco and S. elongatus Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    R. eutropha CbbLS and S. elongatus Prk
    

    Use

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFE-Rs-FI-prk

    Plasmid

    #162697

    Purpose

    Expresses R. spaeroides Form IC rubisco and S. elongatus Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    R. spaeroides Form IC rubisco and S. elongatus Prk
    

    Use

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge