We narrowed to 9,766 results for: 158

1. pJT301 BxBi Lenti pEF-attB LSR GFP

   Plasmid

   #184944

   Purpose

   Lentiviral delivery of recombinase landing pad

   Depositor

   Lacra Bintu, Michael Bassik, Patrick Hsu, Ami Bhatt

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   BxBi Lenti pEF1a-attB LSR GFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRN120

   Plasmid

   #160696

   Purpose

   BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, CmYLCV::STM::35S terminator, Nos::WUS2::PinII terminator

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Inserts

   CmYLCV::Luc+::AtHSP
   Nos::WUS2::PinII
   AtUbi10::STM::35S
   

   Use

   Luciferase; Gemini viral replicon, plant developm…

   Tags

   Expression

   Mutation

   Promoter

   AtUbi10, CmYLCV, and Nos

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRE-Lepr_FL-IRES-eGFP

   Plasmid

   #196635

   Purpose

   Doxycycline inducible expression of full length mouse Lepr with eGFP marker

   Depositor

   Elaine Fuchs

   Article

   Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

   Insert

   Lepr (Lepr Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. CGBE1 (pRZ3885)

   Plasmid

   #140252

   Purpose

   CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   eUNG-BE4max(R33A)-ΔUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, D10A in Cas9

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. HTR1A-Tango

   Plasmid

   #66404

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   HTR1A (HTR1A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-GGamma1-GFP2

   Plasmid

   #140989

   Purpose

   Encodes a G gamma subunit (GNGT1) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma1-GFP2 (GNGT1 Human)
   

   Use

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-Beta2

   Plasmid

   #166772

   Purpose

   Encodes a Gbeta subunit as a "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GNB2 (GNB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-Beta4

   Plasmid

   #166773

   Purpose

   Encodes a Gbeta subunit as a "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GNB4 (GNB4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. CGBE1-NG (pBM1246)

   Plasmid

   #140256

   Purpose

   CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   eUNG-BE4max(R33A)_NG-ΔUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-GGamma8-GFP2

    Plasmid

    #140990

    Purpose

    Encodes a G gamma subunit (GNG8) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma8-GFP2 (GNG8 Human)
    

    Use

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFRT/TO/HIS/FLAG/HA-KIAA1967

    Plasmid

    #38082

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    KIAA1967 (CCAR2 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK5-Myc-PSK1-beta

    Plasmid

    #197121

    Purpose

    Expression of MYC-tagged PSK1-beta in mammalian cells

    Depositor

    Jonathan Morris

    Article

    Zihni et al J Biol Chem. 2007 Mar 2;282(9):6484-93.

    Insert

    TAOK2 variant beta (aa1-1049) (TAOK2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Wild Type variant beta

    Promoter

    SV40

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBABE puro SDF-1 alpha

    Plasmid

    #12270

    Depositor

    Bob Weinberg

    Article

    Orimo et al Cell. 2005 May 6. 121(3):335-48.

    Insert

    SDF-1 alpha (CXCL12 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-cFos-XRI-V5

    Plasmid

    #178058

    Purpose

    Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with V5 tag, under c-fos promoter.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    XRI-V5
    

    Use

    AAV

    Tags

    V5-MBP

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse c-fos promoter

    Available since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Ef1a-MCP-huADARE488QT490A

    Plasmid

    #159922

    Purpose

    Fusion of MS2 coat protein to Human ADAR2 E488QT490A, under Ef1a promoter, with WPRE. AKA 116v5

    Depositor

    Fei Chen

    Article

    Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

    Insert

    Human ADAR2 E488QT490A (ADARB1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAPM-miR30-IFIH1-ts3

    Plasmid

    #174261

    Purpose

    IFIH1 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    IFIH1 shRNA (IFIH1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT7-G-AsCas12a crRNA-BsaI cassette (MSP3491)

    Plasmid

    #114067

    Purpose

    T7 promoter crRNA entry vector used for in vitro transcription of AsCas12a guides

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for AsCas12a crRNAs
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCbs FlagOmomyc

    Plasmid

    #113168

    Purpose

    Expression of FLAG tagged Omomyc in mammalian cells

    Depositor

    Sergio Nasi

    Article

    Savino et al PLoS One. 2011;6(7):e22284. doi: 10.1371

    Insert

    Omomyc (MYC Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E57T, E64I, R70Q, R71N

    Promoter

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX304-FOS-V5

    Plasmid

    #59140

    Purpose

    wild-type FOS with C-terminal V5 tag expressed from pLX vector

    Depositor

    William Hahn

    Article

    Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

    Insert

    FOS (FOS Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-eNme2-T.2-ABE8e

    Plasmid

    #185669

    Purpose

    Expresses eNme2-T.2-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-neNme2-T.2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2Cas9 D16A/E47K/R63K/V68M/A116T/T123A/D152N/E1…

    Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCMV-dCas13-inactive M3nls

    Plasmid

    #157854

    Purpose

    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A; METTL3 D395A

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCDNA5-LAP-MPS1-WT

    Plasmid

    #114044

    Purpose

    Expression of exogenous LAP tagged MPS1 WT

    Depositor

    Geert Kops

    Article

    Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

    Insert

    MPS1-WT (TTK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT MPS1 sequence

    Promoter

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MC2R-Tango

    Plasmid

    #66428

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    MC2R (MC2R Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pRS0045

    Plasmid

    #131124

    Purpose

    Mammalian Target-ACE expression vector

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
    

    Use

    CRISPR

    Tags

    PmCDA1, TadA, and UGI

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V …

    Promoter

    CMV promoter

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1/Myc-His beta4

    Plasmid

    #16039

    Depositor

    Filippo Giancotti

    Article

    Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

    Insert

    beta4 (ITGB4 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TFORF2108

    Plasmid

    #143338

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    L3MBTL4 (L3MBTL4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HA-YAP-wt

    Plasmid

    #229412

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    YAP-wt (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCLXEBR-pTF-cKit

    Plasmid

    #114293

    Purpose

    TET-inducible expression of c-Kit

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Insert

    cKit (Kit Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28b(+)_rPD-L1nb

    Plasmid

    #226715

    Purpose

    Heterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    Recombinant PD-L1 nanobody
    

    Use

    Tags

    HA tag and Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Circular 200,100 (mPCSK9)

    Plasmid

    #170122

    Purpose

    AAV vector carrying 2 copies of a guide RNA targeting the mouse PCSK9 mRNA, expressed from human and mouse U6 promoters

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA (Pcsk9 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 and mouse U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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