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pMK-Cas9-gate

Plasmid

#113743

Purpose

Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving aminoglycoside phosphotransferase for G418 selection in plants.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

Cas9

Use

CRISPR and Gateway Cloning

Promoter

Z. Mays ubiquitin promoter, 35S promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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amilGFP chromoprotein promoter-less

Plasmid

#118006

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

RBS, amilGFP

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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LacI-pLacI-pII pm

Plasmid

#218099

Purpose

Phagemid that expresses LacI, and pII under the Lac promoter

Depositor

Kevin Esvelt

Article

Gretton et al bioRxiv 2024.03.20.585782

Insert

pII

Expression

Bacterial

Promoter

Lac promoter

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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tsPurple chromoprotein promoter-less

Plasmid

#118007

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

RBS, tsPurple

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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DT049A

Plasmid

#159410

Purpose

Constitutive murine mature BDNF expression, controlled under E.coli tet promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.tet-BDNF (Bdnf, Mouse) (Bdnf Mouse, Synthetic)

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

tet promoter

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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promoter J23110 (upstream of RBS, mRFP1)

Plasmid

#117852

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

medium-high promoter, RBS, mRFP1

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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psgRNA (GB0645)

Plasmid

#68210

Purpose

Provides a Short guide RNA which is the combination of the bacterial crRNA and tracrRNA into a single guide transcript; it does not contain any target site

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SgRNA

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pISH-Hopx

Plasmid

#74347

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Hopx. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

HOP homeobox (Hopx Mouse)

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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Gcm2TEASY

Plasmid

#41031

Depositor

Nancy Manley

Article

Gordon et al Mech Dev. 2001 May;103(1-2):141-3.

Insert

Gcm2 in situ probe (Gcm2 Mouse)

Use

In situ probe

Mutation

contains nucleotides 584-1510 of GenBank accessio…

Promoter

T7 sense; SP6 antisense

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only
phumanCas9 (GB0575)

Plasmid

#68206

Purpose

Provides the human codon optimized CDS of Cas9 protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Cas9 coding region (human codon optimised)

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed; human codon optimis…

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pTNos:BASTA:PNos (GB0023)

Plasmid

#68211

Purpose

Provides the bar gene for resistance to basta fused to the A. tumefaciens Nopaline synthase promoter and terminator. Cloned as a level 0 GB part ready to be transferred to a GB level1 plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

bar gene

Use

Unspecified

Mutation

BsaI and BsmBI sites removed

Promoter

PNos

Available Since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pISH-Gnas

Plasmid

#105483

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2014 Jan;58:1-10. doi

Insert

Gnas (Gnas Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLacIBD::SDRX (GB0673)

Plasmid

#68183

Purpose

Provides the LacI Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

LacI Binding Domain fused to SRDX Transcriptional Repressor

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pTARGEX-VAC

Plasmid

#242450

Purpose

Targets high levels of recombinant protein to vacuole. Empty vector. Clone via SapI restriction site. mCherry dropout cassette will enable Red/White screening in E.coli.

Depositor

Allyson MacLean

Article

Chartrand-Pleau et al Front. Synth. Biol. 3:1619871

Type

Empty backbone

Tags

C-terminal propeptide (CTPP) derived from N. taba…

Expression

Plant

Promoter

double 35S

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pVS2-GhCLA

Plasmid

#234906

Purpose

This all-in-one vector is used to specifically silence the GhCLA gene via virus-induced gene silencing (VIGS).

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

VIGS fragement of GhCLA gene

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVS2-NbPDS

Plasmid

#234908

Purpose

This all-in-one vector is used to specifically silence the NbPDS gene via virus-induced gene silencing (VIGS).

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

VIGS fragement of NbPDS gene

Expression

Plant

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pVM2-GoPGF-HD1

Plasmid

#234909

Purpose

This all-in-one vector is used to specifically silence the GoPGF and HD1 gene via virus-induced gene silencing (VIGS), simultaneously.

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

VIGS fragements of GoPGF and GhHD1

Expression

Plant

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pVS2-GoPGF

Plasmid

#234907

Purpose

This all-in-one vector is used to specifically silence the GoPGF gene via virus-induced gene silencing (VIGS).

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

VIGS fragement of GoPGF gene

Expression

Plant

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pVS-GhPDS

Plasmid

#234897

Purpose

This all-in-one vector is used to specifically silence the GhPDS gene via virus-induced gene silencing (VIGS).

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

VIGS fragement of GhPDS gene

Expression

Plant

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pVS-GhOMT1_2

Plasmid

#234904

Purpose

This all-in-one vector is used to specifically silence the GhOMT1 gene via virus-induced gene silencing (VIGS).

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

VIGS fragement 2 of GhOMT1 gene

Expression

Plant

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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