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53BP1 N-terminal sgRNA

Plasmid

#207091

Purpose

pX330 based plasmid for expression of Cas9 and the GGGGAGCAGATGGACCCTAC sgRNA to target the 53BP1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GGGGAGCAGATGGACCCTAC

Expression

Mammalian

Promoter

CMV and U6

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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LP376

Plasmid

#90200

Purpose

pCMV-myc with cDNA encoding HsTRAPPC10 aa residues 1000-1259

Depositor

Lotte Pedersen

Article

Schou et al Cilia. 2014 Jun 18;3:6. doi: 10.1186/204

Insert

TRAPPC10 (TRAPPC10 Human)

Tags

Myc

Expression

Mammalian

Mutation

aa residues 1000-1259

Promoter

CMV

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-mE6-AP/2591a

Plasmid

#11468

Depositor

Allan Weissman

Article

Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

Insert

mE6-AP (Ube3a Mouse)

Tags

myc

Expression

Mammalian

Mutation

n/a

Available Since

March 8, 2006

Availability

Academic Institutions and Nonprofits only
Lenti-EF1alpha-scFv-VP64_Blast

Plasmid

#192655

Purpose

3rd generation lenti vector encoding scFv-VP64 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

scFv-VP64

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-TO-DsRed

Plasmid

#16340

Depositor

Anton Bennett

Article

Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

Insert

DsRed

Expression

Mammalian

Mutation

5'Prime Site: Not I 3'Primer Site : No…

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-4T2 Parkin RBR

Plasmid

#31255

Depositor

Rachel Klevit

Article

Wenzel et al Nature. 2011 Jun 2;474(7349):105-8. doi:

Insert

Parkin (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

RBR domain, residues 217-465

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
pLenti-Arch-EEQ

Plasmid

#45188

Purpose

Arch voltage indicator with double mutations D95Q-D106E (Arch-EEQ), with faster kinetics and greater fluorescence dynamic range than Arch-D95N

Depositor

Mark Schnitzer

Article

Gong et al PLoS One. 2013 Jun 19;8(6):e66959.

Insert

enhanced Archaerhodopsin

Use

Lentiviral

Tags

eYFP

Expression

Mammalian

Mutation

D95Q, D106E

Promoter

CamKIIa

Available Since

July 18, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV10-3xFLAG-prs-MCP1

Plasmid

#184341

Purpose

Express yeast MCP1 in Expi293F cells

Depositor

Karin Reinisch

Article

Adlakha et al J Cell Biol. 2022 Jun 6;221(5):e20220203

Insert

Yeast MCP1 (MCP1 Budding Yeast)

Tags

3xFLAG-PreScission cleavage site

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR APLP2 shRNA 2

Plasmid

#30160

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al Neural Dev. 2008 Jun 23;3:15. doi: 10.11

Insert

APLP2 shRNA 2

Expression

Mammalian

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
Filamin-M-stFRET

Plasmid

#61105

Purpose

Expression of Filamin with internal stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

filamin-stFRET

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pBB14

Plasmid

#18657

Depositor

Lynn Enquist

Article

Brideau et al J Virol. 1998 Jun . 72(6):4560-70.

Insert

Us9-EGFP gene fusion

Tags

EGFP

Expression

Mammalian

Mutation

First 106 amino acids of Us9 are intact. The stop…

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pAG506-mito-frFAST

Plasmid

#160057

Purpose

Expresses mito-frFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

Mito-frFAST

Expression

Mammalian

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGR-BBa_B0010

Plasmid

#46003

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

BBa_B0010 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available Since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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TRPML2-HA

Plasmid

#18828

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML2 (Mcoln2 Mouse)

Tags

HA

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
Actinin-M-stFRET

Plasmid

#61103

Purpose

Expression of α-actinin with internal stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

α-actinin-stFRET

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-HTB-kanMX6

Plasmid

#26875

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HTB tag

Use

Pcr tagging yeast

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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Venus-KRasCT

Plasmid

#99016

Purpose

Membrane marker

Depositor

Narasimhan Gautam

Article

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Insert

Venus KRasCT

Expression

Mammalian

Promoter

CM

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pJP118

Plasmid

#176494

Purpose

Expresses Lifeact-mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoter

Depositor

Duojia Pan

Article

Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

Insert

Lifeact peptide

Use

Capsaspora owczarzaki expression

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP N1 Ub-CRT

Plasmid

#69619

Purpose

Express cytoplasmic mature CRT.

Depositor

Marta Hallak

Article

Goitea et al J Biol Chem. 2015 Jun 26;290(26):16403-1

Insert

Ubiquitina-Calreticulin (mature)

Tags

EGFP and Ubiquitin

Expression

Mammalian

Mutation

Deletion of signal peptide of calreticulin at the…

Promoter

CMV

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pF3BGX-TangoVL-lexA

Plasmid

#138406

Purpose

RMCE vector for generating C-terminal Tango fusion

Depositor

Shu Kondo

Article

Katow et al J Neurogenet. 2019 Mar - Jun;33(2):44-51

Insert

V2 tail-TEVcs(ENLYFQL)-LexAp65

Expression

Insect

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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