Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 16,181 results for: nes

Clear keywords
Showing: 2641 - 2660 of 16181 results

  11. BB3cH_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104907
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Jan. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    104907-plasmid-map-sequence-id-206042

    Enlarge

  13. pMM441

    Plasmid
    #61271
    Purpose
    Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.
    Depositor
    Timothy Lu
    Publication
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    61271-plasmid-map-sequence-id-99582

    Enlarge

  14. pRluc-LC3G120A

    Plasmid
    #105003
    Purpose
    Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.
    Depositor
    Marja Jããttelã
    Publication
    Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LC3B (Map1lc3b Rat)
    Use
    Luciferase
    Tags
    Rluc with C124A substitution
    Expression
    Mammalian
    Mutation
    Glycine 120 mutated to alanine, prevents lipidati…
    Available since
    Jan. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    105003-plasmid-map-sequence-id-202164

    Enlarge

Showing: 2641 - 2660 of 16181 results