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pV231-xPE

Plasmid

#225257

Purpose

Mammalian expression of xPE

Depositor

Vikash Chauhan, Robert Langer, Phil Sharp

Article

Chauhan et al Nature. 2025 Sep 17. doi: 10.1038/s41586

Insert

xPE

Use

CRISPR

Expression

Mammalian

Mutation

R221K K848A H982A N1317R

Promoter

CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pKanCMV-mClover3-10aa-H2B

Plasmid

#74257

Purpose

Mammalian expression of N-terminal fusion of mClover3 to H2B

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

H2B

Tags

mClover3

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-FASCIN

Plasmid

#31207

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

FASCIN (FSCN1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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PKC eta WT

Plasmid

#21244

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC eta (Prkch Mouse)

Tags

HA

Expression

Mammalian

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only
pMM441

Plasmid

#61271

Purpose

Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.

Depositor

Timothy Lu

Article

Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

Insert

Cas9, tracrRNA, crRNA

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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PKC alpha dNPS

Plasmid

#21233

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC alpha (PRKCA Human)

Tags

HA

Expression

Mammalian

Mutation

delta 1-29

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only
SPACE-NG (pRZ5150)

Plasmid

#140244

Purpose

CMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

SPACE_NG

Expression

Mammalian

Mutation

V82G in TadA*, NG mutations in SpCas9, D10A in Sp…

Promoter

CMV

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P34

Plasmid

#120030

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xFLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xFLAG

Use

Pcr template

Tags

3xFLAG

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCS-membrane-ceruleanFP

Plasmid

#53749

Purpose

Contains membrane localized cerulean fluorescent protein in pCS expression vector.

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

2x membrane linker

Tags

Cerulean

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pBID-UASC-FG

Plasmid

#35201

Depositor

Brian McCabe

Article

Wang et al PLoS One. 2012;7(7):e42102. Epub 2012 Ju

Type

Empty backbone

Use

Drosophila transgene expression

Tags

3XFlag

Promoter

Drosophila Synthetic Core Promoter (DSCP)

Available Since

July 26, 2012

Availability

Academic Institutions and Nonprofits only

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CaV1.3e[8a,11,31b,Δ32,42a] mut

Plasmid

#26576

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

Contains exons 8a, 11, 31b and 42a.Deletion of ex…

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only
pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

Plasmid

#183411

Purpose

piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.

Depositor

Azim Surani

Article

Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

Insert

Tet-on 3G

Use

CRISPR and Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

EF1A

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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TagRFP-T-Rabenosyn-5

Plasmid

#37537

Depositor

Silvia Corvera

Article

Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

Insert

Rabenosyn5 (RBSN Human)

Tags

TagRFP-T

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2013

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf106

Plasmid

#12794

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf106

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pRS415_pGal-nCas9(D10A)

Plasmid

#79618

Purpose

Expresses nCas9(D10A) in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Expression

Yeast

Mutation

D10A for nickase Cas9

Promoter

pGal1

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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3ME6/mSprouty2

Plasmid

#22097

Depositor

Gail Martin

Article

Minowada et al Development. 1999 Oct . 126(20):4465-75.

Insert

Sprouty 2 (Spry2 Mouse)

Use

Bacterial

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA6 asyn A30P

Plasmid

#105729

Purpose

Mammalian expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Article

Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

Insert

alpha-synuclein A30P (SNCA Human)

Expression

Mammalian

Mutation

A30P

Promoter

CMV

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pJFA334E9

Plasmid

#49237

Purpose

TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells

Depositor

Keith Joung

Article

Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

Insert

TET1 tet methylcytosine dioxygenase 1 (TET1 Human, Synthetic)

Use

TALEN; Tale-tet1 expression vector

Promoter

EF1a

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pDicAID_nCas9-PmCDA-2A-NptII_ETR

Plasmid

#91695

Purpose

Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr

Depositor

Akihiko Kondo

Article

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

PcUbi

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pSuperRetropuro-IL-6 scramble

Plasmid

#19960

Depositor

Christopher Counter

Article

Ancrile et al Genes Dev. 2007 Jul 15. 21(14):1714-9.

Insert

pSuperRetropuro-IL-6 scramble

Use

RNAi and Retroviral

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only