We narrowed to 1,459 results for: Mbp

1. pMAL c2x Tral C

   Plasmid

   #113008

   Purpose

   For bacterial expression of MBP fusion of C terminal region of Drosophila Tral

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   C terminus of tral (tral Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   amino acids 356-652

   Promoter

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJSC119 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant

   Plasmid

   #101207

   Purpose

   Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant N497A/R661A/Q695A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   N497A, R661A and Q695A

   Promoter

   T7

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJSC052 - Bacterial expression plasmid for SpCas9, REC3 FRET variant

   Plasmid

   #101201

   Purpose

   Bacterial expression plasmid for SpCas9, REC3 FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S701C/S960C
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S701C and S960C

   Promoter

   T7

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJSC077 - Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

   Plasmid

   #101214

   Purpose

   Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S355C/S867C/K855A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S355C, S867C and K855A

   Promoter

   T7

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant

   Plasmid

   #101200

   Purpose

   Bacterial expression plasmid for SpCas9, REC2 FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/E60C/D273C
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, E60C and D273C

   Promoter

   T7

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. hSRAP.HMG6.wt.Dlnk.a13-236

   Plasmid

   #49814

   Purpose

   expression vector for MBP-SRAP(13-236) fusion

   Depositor

   Thomas Cech

   Article

   McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

   Insert

   steroid receptor RNA activator protein (SRA1 Human)
   

   Use

   Tags

   E. coli Maltose Binding Protein

   Expression

   Bacterial

   Mutation

   wildtype codon-optimized for E. coli expression

   Promoter

   T7

   Available since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET 2C-T10-dCasX

   Plasmid

   #126418

   Purpose

   E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Insert

   DpbCasX
   

   Use

   CRISPR

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDF-rM1PYK

   Plasmid

   #220232

   Purpose

   Inducible expression of rabbit muscle pyruvate kinase isoform 1

   Depositor

   Aron Fenton, Liskin Swint-Kruse

   Article

   Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

   Insert

   Rabbit muscle pyruvate kinase (PKM )
   

   Use

   Tags

   6xHis-MBP-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMAL-XPG

   Plasmid

   #39215

   Depositor

   Aziz Sancar

   Article

   Matsunaga et al J Biol Chem. 1995 Sep 1;270(35):20862-9.

   Insert

   XPG (ERCC5 Human)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Note that XPG starts at amino acid 5

   Promoter

   Ptac

   Available since

   Aug. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac1-LRRK2 WD40

    Plasmid

    #139211

    Purpose

    Human LRRK2 WD40 domain (residues 2142–2527) was expressed in Sf9 insect cells via baculovirus infection and purified to homogeneity.

    Depositor

    Hao Wu

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2019 Jan 29;11

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    N-His and MBP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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