Purpose
Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1.
Mutation
N497A; R661A; Q695A; Q926A; amino acids 1005-1013…
Available Since
March 6, 2020
Availability
Academic Institutions and Nonprofits only