We narrowed to 120,297 results for: CIL

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pCRISPR-PsppA-mCherry

Plasmid

#225428

Purpose

Plasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherry

Depositor

Geir Mathiesen

Article

Wiull et al Microbiol Spectr. 2025 Feb 4;13(2):e0202

Insert

mCherry, Cas9, SgRNA

Use

CRISPR

Expression

Bacterial

Promoter

PsppA upstream of mCherry and Cas9, P3 upstream o…

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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S12.2_Bridge + Target

Plasmid

#127671

Purpose

Ampicillin resistant multiplexing backbone with CRY2HA and mCherry

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Type

Empty backbone

Use

CRISPR

Tags

HA

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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YE1-BE4max

Plasmid

#138155

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YE1)-nSpCas9-UGI-UGI

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R126E; within Cas9 D10A

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pShuttle-FEN1DA

Plasmid

#35028

Depositor

Sheila Stewart

Article

Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

Insert

FEN1DA (FEN1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

D181A--NUCLEASE DEAD POINT MUTANT

Promoter

CMV

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
pET28a-MSP1D1deltaH4H5

Plasmid

#71716

Purpose

Membrane scaffold protein (MSP), helix 4+ helix 5 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Expression

Bacterial

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pShuttle-FEN1deltaP

Plasmid

#35030

Depositor

Sheila Stewart

Article

Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

Insert

FEN1DELTAP (FEN1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

8 AMINO ACID DELETION OF P BOX (amino acids 337–3…

Promoter

CMV

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
pEGFPN3-5ht6

Plasmid

#35624

Depositor

Kirk Mykytyn

Article

Berbari et al Mol Biol Cell. 2008 Apr;19(4):1540-7. Ep

Insert

5ht6 (Htr6 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pAcCas12nHS_RV6_empty

Plasmid

#203811

Purpose

Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcCas12nHS_RV6

Use

CRISPR

Promoter

CMV

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-CAG-empty-ires-EGFP

Plasmid

#122953

Purpose

Lentiviral expression empty vector under CAG promoter with EGFP expression

Depositor

Pierre Vanderhaeghen

Article

Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

Type

Empty backbone

Use

Lentiviral

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pSNA

Plasmid

#135192

Purpose

Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains ampicillin resistance cassette

Depositor

Hongqing Fang

Article

Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

Insert

I-SceI lamba Red

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Promoter

araBAD

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1deltaH4-H6

Plasmid

#71717

Purpose

Membrane scaffold protein (MSP), helix 4 to 6 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Expression

Bacterial

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-UBE4B

Plasmid

#113499

Purpose

Expression of human UBE4B with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

UBE4B (UBE4B Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Cep164 (Nigg CW324)

Plasmid

#41149

Depositor

Erich Nigg

Article

Graser et al J Cell Biol. 2007 Oct 22;179(2):321-30.

Insert

Cep164 (CEP164 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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pAcCas12nHS_RV6_PT1_empty

Plasmid

#203810

Purpose

Plasmid containing a CMV-driven NLS-AcCas12n-PT1-NLS(human-codonoptimized)cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion). Carbenicillin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcCas12nHS_RV6_PT1

Use

CRISPR

Promoter

CMV

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDonor-tBFP-NLS-Neo

Plasmid

#80766

Purpose

Customizable donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Type

Empty backbone

Use

CRISPR

Tags

TagBFP2-3×NLS

Expression

Mammalian

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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YE2-BE4max

Plasmid

#138156

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YE2)-nSpCas9-UGI-UGI

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R132E; within Cas9 D10A

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLPP6

Plasmid

#209968

Purpose

Contains Level 0 Part: Promoter Dropout (sfGFP) for the construction of Level 1 plasmids and subsequent promoter insertion

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter Dropout (sfGFP)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pSNRW-Z5-Nluc

Plasmid

#174487

Purpose

Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

Depositor

Richard C. Willson

Article

Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

Insert

Z5-Nluc

Tags

6x Histidine tag (N terminal on insert)

Expression

Bacterial

Promoter

T7lac promoter

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pMy-BirA-T2A-eGFP

Plasmid

#50565

Purpose

Retroviral vector encoding for GFP and the biotinylating enzyme BirA

Depositor

Rafael Casellas

Article

Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

Insert

Ampicilin

Use

Retroviral

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pGI3EM17

Plasmid

#210569

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

SpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter

Use

Luciferase

Tags

NanoLuc luciferase

Expression

Bacterial and Yeast

Promoter

SpH2Bpro

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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