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Plasmid#105776PurposeExpression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)DepositorTypeEmpty backboneUseFlp-in competentTagsEGFP-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only
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pcDNA3-sACE2-WT(732)
Plasmid#154098PurposeMammalian expression plasmid for dimeric soluble ACE2 (untagged)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsExpressionMammalianMutationExtracellular, soluble protease and neck domains …PromoterCMVAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas9-mD3A
Plasmid#78257PurposeExpresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) under the control of the CMV promoterDepositorInsertDNMT3A CD aa 598-912 (DNMT3A Human)
UseCRISPRTagsFlag epitope tagExpressionMammalianMutationE756APromoterpCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKK-mTagBFP-TEV
Plasmid#105772PurposeExpression of your protein of interest in fusion with blue fluorescent protein at the N-terminus (cleavable by TEV) (PMID: 18940671, 25378625). Useful for multi-channel live cell imaging.DepositorTypeEmpty backboneUseFlp-in competentTagsmTagBFP-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75236PurposeCRISPR/Cas9 plasmid against human NFATc1DepositorInsertsgRNA against NFATc1 (NFATC1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-FOSB
Plasmid#192901PurposeBarcoded piggybac transposon vector with Dox-inducible expression of FOSBDepositorInsertFOSB (FOSB Human)
UseTagsHAExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hH4-AVITEV
Plasmid#74051Purposeretroviral expression plasmid for human histone H4 with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman Histone-H4 (H4C16 Human)
UseRetroviralTagsAVI-TEVExpressionMammalianMutationPromoterpMSCV-LTRsAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75237PurposeCRISPR/Cas9 plasmid against human NFATc1DepositorInsertsgRNA against NFATc1 (NFATC1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
NLS-HA-2xMCP-Ezh2
Plasmid#126590PurposeExpresses MCP (MS2 Coat Protein) fusion to Ezh2 in mammalian cells, lentiviral backboneDepositorInsert2XMCP-Ezh2 (Ezh2 Synthetic, Mouse)
UseLentiviralTagsNLS-HAExpressionMammalianMutationPromoterhuman ubiquitin C promoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTE4566
Plasmid#88904PurposeExpresses MbCpf1 crRNA and inactive/dead, humanized MbCpf1 nucleaseDepositorInsertsMb crRNA
hMbCpf1(D986A)
UseCRISPRTags3xHA and NLSExpressionMammalianMutationPromoterCMV and human U6Available sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-JUNB
Plasmid#192907PurposeBarcoded piggybac transposon vector with Dox-inducible expression of JUNBDepositorInsertJUNB (JUNB Human)
UseTagsHAExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mTagBFP
Plasmid#105789PurposeExpression of your protein of interest in fusion with blue fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 18940671, 25378625). Useful for multi-channel live cell imaging.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mTagBFPExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hNFATc1/bC - AVITEV
Plasmid#74059Purposeretroviral expression plasmid for human NFATc1/bC with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc1, isoform beta-C (NFATC1 Human)
UseRetroviralTagsAVI-TEVExpressionMammalianMutationPromoterpMSCV-LTRsAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-LacI-Cyclin B1 ND
Plasmid#234706Purposeallows tethering of non degradable (ND) cyclin B1 protein to lacO repeats in specific chromatin lociDepositorInsertCyclin B1 (CCNB1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-BUBR1
Plasmid#234704Purposeexpression of fusion proteinDepositorInsertBUBR1 (BUB1B Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRRL-mouse STING #1-gRNA-Cas9-Puro
Plasmid#186892PurposegRNA targeting mouse STING (with Cas9 insert)DepositorInsertCas9 (Sting1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRRL-mouse STING #2-gRNA-Cas9-Puro
Plasmid#186893PurposegRNA targeting mouse STING (with Cas9 insert)DepositorInsertCas9 (Sting1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-chimpanzee cGAS-HA
Plasmid#186873PurposeDoxycycline-dependent expression of chimpanzee cGAS gene in mammalian cells by retroviral transductionDepositorInsertChimpanzee cGAS with C-terminal HA tag (CGAS Pan troglodytes)
UseRetroviralTagsHAExpressionMutationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-mouse cGASdeltaN-HA
Plasmid#186876PurposeDoxycycline-dependent expression of mouse cGAS gene in mammalian cells by retroviral transductionDepositorInsertMouse cGAS truncation mutant (148-508 a.a.) with C-terminal HA tag (Cgas Synthetic, Mouse)
UseRetroviralTagsHAExpressionMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-gibbon cGASdeltaN-HA
Plasmid#186861PurposeDoxycycline-dependent expression of gibbon cGAS gene in mammalian cells by retroviral transductionDepositorInsertGibbon cGAS trucation mutant (152-514 a.a.) with C-terminal HA tag (CGAS Synthetic, Hylobatidae)
UseRetroviralTagsHAExpressionMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only