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CMVTO-GFP-ECL-3TM-hcvs-KKMP

Plasmid

#179641

Purpose

HCVP-inducible membrane-bound GFP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Membrane-bound GFP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-SEAP-26sFur-B2AD-hcvs-tevs-KKMP

Plasmid

#179643

Purpose

OR Gate (TEVP/HCVP) SEAP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBD21_035

Plasmid

#109152

Purpose

pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of EGFP

Depositor

Ron Weiss

Article

Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

Inserts

mRFP
EGFP

Use

Synthetic Biology

Expression

Bacterial and Mammalian

Promoter

CMV and pTet

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-tglA(C51)

Plasmid

#134674

Purpose

Expresses His6-TglA-Cys in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

TglA(C51)

Tags

His6

Expression

Bacterial

Mutation

insertion of cysteine 51

Promoter

T7

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-tglA

Plasmid

#134673

Purpose

Expresses His6-TglA in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

TglA

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pACYCDuet1-ammA(∆50-56)

Plasmid

#134684

Purpose

Expresses His6-AmmA(∆50-56) in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammA(del50-56)

Tags

His6

Expression

Bacterial

Mutation

deletion of amino acids 50-56

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pACYCDuet1-ammA

Plasmid

#134682

Purpose

Expresses His6-AmmA in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammA

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-ammB4

Plasmid

#134688

Purpose

Expresses His6-AmmB4 in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammB4

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-ammB3

Plasmid

#134687

Purpose

Expresses His6-AmmB3 in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammB3

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-ammB2

Plasmid

#134686

Purpose

Expresses His6-AmmB2 in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammB2

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-ammB1

Plasmid

#134685

Purpose

Expresses His6-AmmB1 in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammB1

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b-tglF

Plasmid

#134681

Purpose

Expresses His6-TglF in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

TglF

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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MXS_PA-GFP

Plasmid

#62412

Purpose

MXS_chaining vector with PA-GFP

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

PA-GFP

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_loxP

Plasmid

#62430

Purpose

MXS_chaining vector with loxP

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

loxP site

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_linker

Plasmid

#62431

Purpose

MXS_chaining vector with linker

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Linker 12AA Gly-/Ser-rich

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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35S-GAL4BD-GWY/pAM

Plasmid

#201233

Purpose

Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD

Depositor

Michael Ayliffe

Article

Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

Insert

35S-GAL4BD-GWY

Expression

Plant

Promoter

CaMV 35S

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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35S-GWY-GAL4BD/pAM

Plasmid

#201231

Purpose

Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD

Depositor

Michael Ayliffe

Article

Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

Insert

35S-GWY-GAL4BD

Expression

Plant

Promoter

CaMV 35S

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pFYF1320 EGFP Site#1

Plasmid

#47511

Purpose

human gRNA expression vector targeting EGFP

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-EGFP site 1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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miReporter-CAG

Plasmid

#82478

Purpose

microRNA reporter relying on a bidirectional CAG-based promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

Insert

H2B-mCherry and H2B-Citrine. HygroR

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyTag002-MBP

Plasmid

#102831

Purpose

Bacterial expression of SpyTag002 fused to maltose binding protein

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

SpyTag002-MBP

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

Peptide tag added to the N-terminus of MBP via a …

Promoter

T7

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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