We narrowed to 165,685 results for: addgene

1. pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)

   Plasmid

   #185918

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLIK CA RhoA

   Plasmid

   #112894

   Purpose

   promoter-inducible expression of constitutively-active RhoA

   Depositor

   Sanjay Kumar

   Article

   MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

   Insert

   CA RhoA (RHOA Human)
   

   Use

   Lentiviral

   Expression

   Bacterial

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. HLA_DRB1_07_01

   Plasmid

   #165014

   Purpose

   Expression of HLA-DRB1*07:01 cDNA

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   HLA-DRB1*07:01 (HLA-DRB1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. MX2_pCSdest

   Plasmid

   #53942

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   MX2 (MX2 Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLJM1 Flag Raptor Rheb15

   Plasmid

   #26634

   Depositor

   David Sabatini

   Article

   Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

   Insert

   raptor-Rheb15 (RPTOR Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Nov. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVS2

   Plasmid

   #33144

   Depositor

   Michael Rosbash

   Article

   Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

   Inserts

   LacO (256 copies)
   GAL-GFP-kd200-GALpA reporter
   

   Expression

   Yeast

   Mutation

   GFP is mutated such that amino acids 64-73 (FSYGV…

   Promoter

   bidirectional GAL1-GAL10 promoter

   Available Since

   Dec. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pTlpA_mCherry_Txe/Axe

   Plasmid

   #229147

   Purpose

   Toxin-Antitoxin (Txe/Axe)

   Depositor

   Shrikrishnan Sankaran

   Article

   Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

   Insert

   mCherry, Txe/Axe
   

   Expression

   Bacterial

   Promoter

   PtlpA (mCherry), Txe/Axe (Native)

   Available Since

   Jan. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVCT-rtTR-KRAB-2SM2

   Plasmid

   #11779

   Purpose

   Tet-regulated (Tet-off) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

   Depositor

   Didier Trono, Patrick Aebischer

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   CAG promoters, GFP, rtTR-KRAB-2SM2, Tet-off
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. hIRE1a K599A

   Plasmid

   #20745

   Depositor

   Fumihiko Urano

   Article

   Lipson et al PLoS ONE. 2008 . 3(2):e1648.

   Insert

   IRE1 (ERN1 Human)
   

   Use

   Lentiviral; Destination vector

   Expression

   Mammalian

   Mutation

   K599A, dominant negative kinase mutant

   Available Since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

10. USP25_pCSdest

    Plasmid

    #53817

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    USP25 (USP25 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pET28a-HaloTag-CNA35

    Plasmid

    #215457

    Purpose

    Encodes the bacterial collagen-binding protein CNA35 with N-terminal HaloTag.

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    CNA35
    

    Tags

    HaloTag

    Expression

    Bacterial

    Mutation

    Includes stop codon

    Available Since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pCW-SPIC

    Plasmid

    #162825

    Purpose

    Doxycycline-inducible overexpression of human SPIC

    Depositor

    Matthew Steinhauser

    Article

    Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

    Insert

    SPIC (SPIC Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Tet ON

    Available Since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT3TS-miniTurboID-EGFP

    Plasmid

    #163847

    Purpose

    In vitro transcription of miniTurboID-EGFP. Parton lab clone JRT

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    miniTurbo
    

    Use

    In vitro transcription of rna

    Tags

    EGFP

    Available Since

    Sept. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. dCas9 fused to nanobody (anti-beta catenin)

    Plasmid

    #186420

    Purpose

    Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag

    Depositor

    Stephen Elledge

    Article

    Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

    Insert

    dCas9-nanobody fusion (anti-beta catenin)
    

    Use

    CRISPR

    Tags

    6His

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA6a-link-ymTq2-URA3

    Plasmid

    #168057

    Purpose

    Replaces Addgene Plasmid 118458. Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymTurquoise2
    

    Tags

    ymTurquoise2

    Expression

    Yeast

    Available Since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hsyn-GRAB_Ado1.0mut

    Plasmid

    #153287

    Purpose

    Express the adenosine-insensitive control sensor GRAB_Ado1.0mut in neurons

    Depositor

    Yulong Li

    Article

    Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

    Insert

    Adenosine-insensitive sensor GRAB_Ado1.0mut (control sensor)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    July 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT3TS-TurboID-CaaX

    Plasmid

    #163848

    Purpose

    In vitro transcription of TurboID-CaaX. Parton lab clone JSU

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    TurboID
    

    Use

    In vitro transcription of rna

    Tags

    CaaX

    Available Since

    Sept. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT3TS-CD44b-TurboID

    Plasmid

    #163850

    Purpose

    In vitro transcription of CD44b-TurboID. Parton lab clone JTJ

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    CD44b (cd44b Zebrafish)
    

    Use

    In vitro transcription of rna

    Tags

    TurboID

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT3TS-BASU-EGFP

    Plasmid

    #163845

    Purpose

    In vitro transcription of BASU-EGFP. Parton lab clone JRR

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    BASU
    

    Use

    In vitro transcription of rna

    Tags

    EGFP

    Available Since

    Sept. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pdCas9-omega

    Plasmid

    #114632

    Purpose

    Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

    Depositor

    Anushree Chatterjee

    Article

    Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

    Insert

    dCas9-omega
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Tet promoter

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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