We narrowed to 5,211 results for: golden gate

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pSEVAb837

Plasmid

#217501

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb83 backbone, pBBR1 ori

Depositor

Nico Claassens

Publication

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVAb887

Plasmid

#217504

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb88 backbone, pUC ori

Depositor

Nico Claassens

Publication

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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DVK_pBR322_EF

Plasmid

#217594

Purpose

Destination vector with kanamycin resistance and pBR322 origin of replication. Carries E-F CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Publication

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(TCG2)

Plasmid

#235068

Purpose

Expresses Tad-CBE(TCG2) in mammalian cells.

Depositor

Weixin Tang

Publication

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(TCG2)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(CCA3)

Plasmid

#235070

Purpose

Expresses Tad-CBE(CCA3) in mammalian cells.

Depositor

Weixin Tang

Publication

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(CCA3)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(CCC2)

Plasmid

#235072

Purpose

Expresses Tad-CBE(CCC2) in mammalian cells.

Depositor

Weixin Tang

Publication

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(CCC2)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(GCA1)

Plasmid

#235075

Purpose

Expresses Tad-CBE(GCA1) in mammalian cells.

Depositor

Weixin Tang

Publication

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(GCA1)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(GCC3)

Plasmid

#235078

Purpose

Expresses Tad-CBE(GCC3) in mammalian cells.

Depositor

Weixin Tang

Publication

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(GCC3)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(GCG4)

Plasmid

#235080

Purpose

Expresses Tad-CBE(GCG4) in mammalian cells.

Depositor

Weixin Tang

Publication

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(GCG4)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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SpRY-Tad-CBE(ACC2)

Plasmid

#235081

Purpose

Expresses SpRY-Tad-CBE(ACC2) in mammalian cells.

Depositor

Weixin Tang

Publication

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(ACC2)-SpRY Cas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE2.1

Plasmid

#235053

Purpose

Expresses Tad-CBE2.1 in mammalian cells.

Depositor

Weixin Tang

Publication

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE2.1-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE2.4

Plasmid

#235055

Purpose

Expresses Tad-CBE2.4 in mammalian cells.

Depositor

Weixin Tang

Publication

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE2.4-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE3.6

Plasmid

#235058

Purpose

Expresses Tad-CBE3.6 in mammalian cells.

Depositor

Weixin Tang

Publication

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE3.6-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(ACA1)

Plasmid

#235059

Purpose

Expresses Tad-CBE(ACA1) in mammalian cells.

Depositor

Weixin Tang

Publication

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(ACA1)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(ACC3)

Plasmid

#235062

Purpose

Expresses Tad-CBE(ACC3) in mammalian cells.

Depositor

Weixin Tang

Publication

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(ACC3)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(TCC1)

Plasmid

#235066

Purpose

Expresses Tad-CBE(TCC1) in mammalian cells.

Depositor

Weixin Tang

Publication

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(TCC1)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pSIG2

Plasmid

#226909

Purpose

MoClo-compatible vectors for dsRNA production in E. coli

Depositor

Li Hung Chen

Publication

Wu et al Plant Methods. 2025 Jul 11;21(1):96. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7 promoter

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2440_pGEEC536_pShip-UbC-mRuby2-P2A-PuroR-bGH

Plasmid

#239732

Purpose

Plasmid expressing mRuby2 and PuroR with the UbC promoter

Depositor

Kate Galloway

Publication

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pBTK1062

Plasmid

#236186

Purpose

BsaI compatible kanamycin resistance cassette

Depositor

Jeffrey Barrick

Publication

VanDieren et al MicroPubl Biol. 2025 Aug 2;2025:10.17912

Insert

Kanamycin resistance cassette

Expression

Bacterial

Promoter

CP25

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM30

Plasmid

#233502

Purpose

Level 0 plasmid for MoClo, encodes inducible TDH3-tetO2p for K. marxianus

Depositor

Vincent Martin

Publication

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

TDH3-tetO2p

Use

Synthetic Biology

Expression

Yeast

Mutation

addition of two tetracycline operator sequeneces

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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