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We narrowed to 5,457 results for: golden gate

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Showing: 2661 - 2680 of 5457 results

  4. EBOV 4cis

    Plasmid
    #249332
    Purpose
    Integration plasmid encoding EBOV essential replication factors (NP, VP35, VP30, and L protein)
    Depositor
    Amy Kistler
    Insert
    EBOV NP-P2A-VP35-P2A-VP30-P2A-L protein
    Use
    Piggybac donor vector
    Expression
    Bacterial and Mammalian
    Mutation
    the EBOV NP, VP35, and VP30 ORF sequences within …
    Promoter
    CAG
    Available since
    Jan. 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    249332-plasmid-map-sequence-id-497977

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  11. pEvolvR-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249069
    Purpose
    Expresses nucleus-localized nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249069-plasmid-map-sequence-id-496321

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  12. pEvolvR-mCherry-Slug-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249078
    Purpose
    Expresses nucleus-localized Slug-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    Slug-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249078-plasmid-map-sequence-id-496311

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  13. pEvolvR-mCherry-enCas9(D10A)-PolI3M

    Plasmid
    #249061
    Purpose
    Expresses nucleus-localized enCas9 (D10A) fused to PolI3M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    enCas9-PolI3M
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    249061-plasmid-map-sequence-id-495618

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  14. pEvolvR-mCherry-enCas9(H840A)-PolI5M

    Plasmid
    #249064
    Purpose
    Expresses nucleus-localized enCas9 (H840A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    enCas9(H840A)-PolI5M
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    249064-plasmid-map-sequence-id-495619

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  15. pNDMS291

    Plasmid
    #248098
    Purpose
    sfCFP fluorescent reporter for synthetic Rhizobium nifH promoter
    Depositor
    Barney Geddes
    Insert
    sfCFP
    Expression
    Bacterial
    Promoter
    Rlv_PsnifH
    Available since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    248098-plasmid-map-sequence-id-495377

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  16. pNDMS290

    Plasmid
    #248097
    Purpose
    sfYFP fluorescent reporter for synthetic Rhizobium nifH promoter
    Depositor
    Barney Geddes
    Insert
    sfYFP
    Expression
    Bacterial
    Promoter
    Rlv_PsnifH
    Available since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    248097-plasmid-map-sequence-id-495376

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  19. RcP9

    Plasmid
    #242157
    Purpose
    Recombineering Plasmid. ITPG-induced expression of Recombitron operon (ccdB dropout cassette in Eco1 Retron); PSP-Tet promoter for Gene III induction upon M13 phage infection and aTc addition.
    Depositor
    Dominik Niopek
    Publication
    Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:
    Type
    Empty backbone
    Expression
    Bacterial
    Available since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    242157-plasmid-map-sequence-id-494263

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Showing: 2661 - 2680 of 5457 results